Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection
Fusarium wilt, which affects common bean all across the world, is caused by Fusarium oxysporum f. sp. Phaseoli (Fop). It is necessary to have functional genes in response to Fop infection because they might be used to manage disease. As a crucial regulator, TGA-binding transcription factor (TGA) is...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1137634/full |
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| author | Yu Liu Yu Liu Yu Liu Yuning Huang Yuning Huang Zhao Li Ming Feng Ming Feng Weide Ge Weide Ge Chao Zhong Renfeng Xue Renfeng Xue |
| author_facet | Yu Liu Yu Liu Yu Liu Yuning Huang Yuning Huang Zhao Li Ming Feng Ming Feng Weide Ge Weide Ge Chao Zhong Renfeng Xue Renfeng Xue |
| author_sort | Yu Liu |
| collection | DOAJ |
| description | Fusarium wilt, which affects common bean all across the world, is caused by Fusarium oxysporum f. sp. Phaseoli (Fop). It is necessary to have functional genes in response to Fop infection because they might be used to manage disease. As a crucial regulator, TGA-binding transcription factor (TGA) is engaged in the defense mechanism of plants against pathogens. The role of TGA regulators in common bean in response to Fop infection, however, has not been documented. Hence, we performed genome-wide identified and characterized eight TGA genes in common bean. In this study, eight PvTGA genes were distributed on six chromosomes and classified into four subgroups. The PvTGA genes have the same conserved bZIP and DOG1 domains, but there are specific sequence structures in different PvTGAs. Phylogenetic and synteny analysis explained that PvTGA gene has a close genetic relationship with legume TGAs and that PvTGA03 and PvTGA05 may play an important role in evolution. Transcriptome data explained that expression levels of PvTGA genes showed diversity in different tissues. After Fop inoculation, the expression levels of PvTGA03 and PvTGA07 were significantly different between resistant and susceptible genotypes. Under SA treatment, the expression levels of PvTGA03, PvTGA04, PvTGA06, PvTGA07 and PvTGA08 were significantly different. These results imply that PvTGA03 and PvTGA07 play key roles in SA-mediated resistance to Fusarium wilt. Together, these findings advance knowledge of the PvTGA gene family in common bean and will help future studies aimed at reducing Fusarium wilt. |
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| language | English |
| last_indexed | 2024-04-10T20:50:36Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-c846be31af6e4166950bcc1dc76132e52023-01-23T13:43:14ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011410.3389/fgene.2023.11376341137634Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infectionYu Liu0Yu Liu1Yu Liu2Yuning Huang3Yuning Huang4Zhao Li5Ming Feng6Ming Feng7Weide Ge8Weide Ge9Chao Zhong10Renfeng Xue11Renfeng Xue12College of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Shenyang, ChinaFusarium wilt, which affects common bean all across the world, is caused by Fusarium oxysporum f. sp. Phaseoli (Fop). It is necessary to have functional genes in response to Fop infection because they might be used to manage disease. As a crucial regulator, TGA-binding transcription factor (TGA) is engaged in the defense mechanism of plants against pathogens. The role of TGA regulators in common bean in response to Fop infection, however, has not been documented. Hence, we performed genome-wide identified and characterized eight TGA genes in common bean. In this study, eight PvTGA genes were distributed on six chromosomes and classified into four subgroups. The PvTGA genes have the same conserved bZIP and DOG1 domains, but there are specific sequence structures in different PvTGAs. Phylogenetic and synteny analysis explained that PvTGA gene has a close genetic relationship with legume TGAs and that PvTGA03 and PvTGA05 may play an important role in evolution. Transcriptome data explained that expression levels of PvTGA genes showed diversity in different tissues. After Fop inoculation, the expression levels of PvTGA03 and PvTGA07 were significantly different between resistant and susceptible genotypes. Under SA treatment, the expression levels of PvTGA03, PvTGA04, PvTGA06, PvTGA07 and PvTGA08 were significantly different. These results imply that PvTGA03 and PvTGA07 play key roles in SA-mediated resistance to Fusarium wilt. Together, these findings advance knowledge of the PvTGA gene family in common bean and will help future studies aimed at reducing Fusarium wilt.https://www.frontiersin.org/articles/10.3389/fgene.2023.1137634/fullCommon beanTGA gene familyGene familyFusarium oxysporum f. sp. PhaseoliFusarium wilt |
| spellingShingle | Yu Liu Yu Liu Yu Liu Yuning Huang Yuning Huang Zhao Li Ming Feng Ming Feng Weide Ge Weide Ge Chao Zhong Renfeng Xue Renfeng Xue Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection Frontiers in Genetics Common bean TGA gene family Gene family Fusarium oxysporum f. sp. Phaseoli Fusarium wilt |
| title | Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection |
| title_full | Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection |
| title_fullStr | Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection |
| title_full_unstemmed | Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection |
| title_short | Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection |
| title_sort | genome wide identification of the tga genes in common bean phaseolus vulgaris and revealing their functions in response to fusarium oxysporum f sp phaseoli infection |
| topic | Common bean TGA gene family Gene family Fusarium oxysporum f. sp. Phaseoli Fusarium wilt |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1137634/full |
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