bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29
Abstract Background A carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum. Methods Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays we...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13756-019-0596-1 |
| _version_ | 1818148673996980224 |
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| author | Yi Yuan Ying Li Guangxi Wang Chengwen Li Yung-Fu Chang Wenbi Chen Siji Nian Yingyu Mao Jinping Zhang Fangcai Zhong Luhua Zhang |
| author_facet | Yi Yuan Ying Li Guangxi Wang Chengwen Li Yung-Fu Chang Wenbi Chen Siji Nian Yingyu Mao Jinping Zhang Fangcai Zhong Luhua Zhang |
| author_sort | Yi Yuan |
| collection | DOAJ |
| description | Abstract Background A carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum. Methods Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays were performed using wax moth larvae. The transfer of bla NDM-5 between bacterial strains was tested using conjugation. 59 genome assemblies of ST29 K. pneumoniae and 230 IncX3 plasmids regardless of the carriage of resistance gene were employed for phylogenetic analysis, respectively. Results The strain carried a virulence plasmid pVir-SCNJ1 bearing the virulence gene rmpA and exhibited a high virulence in wax moth. This hypervirulent strain belongs to sequence type 29 and carries bla NDM-5, which is located on a conjugative plasmid, designated pNDM5-SCNJ1, belonging to type IncX3. pNDM5-SCNJ1 was fully sequenced and shows high similarity with pNDM_MGR194, except some deletion inside the ISAba125 region. Phylogenetic analysis of IncX3 plasmids revealed that although bla NDM-5 can be evolved from bla NDM-1 via point mutations within some IncX3 plasmids, most of bla NDM-5-carrying IncX3 plasmids probably have acquired bla NDM-5 in multiple events. Conclusions In this study, we characterized a bla NDM-5-positive hypervirulent K. pneumoniae of sequence type 29 in China. Our results highlight the need for active surveillance on this lineage of carbapenem-resistant K. pneumoniae. |
| first_indexed | 2024-12-11T12:54:53Z |
| format | Article |
| id | doaj.art-c848e50c4b0e4d84a6bbcf3dca38e914 |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-11T12:54:53Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-c848e50c4b0e4d84a6bbcf3dca38e9142022-12-22T01:06:36ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-08-01811910.1186/s13756-019-0596-1bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29Yi Yuan0Ying Li1Guangxi Wang2Chengwen Li3Yung-Fu Chang4Wenbi Chen5Siji Nian6Yingyu Mao7Jinping Zhang8Fangcai Zhong9Luhua Zhang10Department of Laboratory Medicine, The First People’s Hospital of NeijiangDepartment of Immunology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Immunology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell UniversityDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityDepartment of Laboratory Medicine, The First People’s Hospital of NeijiangDepartment of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical UniversityAbstract Background A carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum. Methods Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays were performed using wax moth larvae. The transfer of bla NDM-5 between bacterial strains was tested using conjugation. 59 genome assemblies of ST29 K. pneumoniae and 230 IncX3 plasmids regardless of the carriage of resistance gene were employed for phylogenetic analysis, respectively. Results The strain carried a virulence plasmid pVir-SCNJ1 bearing the virulence gene rmpA and exhibited a high virulence in wax moth. This hypervirulent strain belongs to sequence type 29 and carries bla NDM-5, which is located on a conjugative plasmid, designated pNDM5-SCNJ1, belonging to type IncX3. pNDM5-SCNJ1 was fully sequenced and shows high similarity with pNDM_MGR194, except some deletion inside the ISAba125 region. Phylogenetic analysis of IncX3 plasmids revealed that although bla NDM-5 can be evolved from bla NDM-1 via point mutations within some IncX3 plasmids, most of bla NDM-5-carrying IncX3 plasmids probably have acquired bla NDM-5 in multiple events. Conclusions In this study, we characterized a bla NDM-5-positive hypervirulent K. pneumoniae of sequence type 29 in China. Our results highlight the need for active surveillance on this lineage of carbapenem-resistant K. pneumoniae.http://link.springer.com/article/10.1186/s13756-019-0596-1Carbapenem resistancebla NDM-5HypervirulentIncX3 |
| spellingShingle | Yi Yuan Ying Li Guangxi Wang Chengwen Li Yung-Fu Chang Wenbi Chen Siji Nian Yingyu Mao Jinping Zhang Fangcai Zhong Luhua Zhang bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 Antimicrobial Resistance and Infection Control Carbapenem resistance bla NDM-5 Hypervirulent IncX3 |
| title | bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 |
| title_full | bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 |
| title_fullStr | bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 |
| title_full_unstemmed | bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 |
| title_short | bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29 |
| title_sort | bla ndm 5 carried by a hypervirulent klebsiella pneumoniae with sequence type 29 |
| topic | Carbapenem resistance bla NDM-5 Hypervirulent IncX3 |
| url | http://link.springer.com/article/10.1186/s13756-019-0596-1 |
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