New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova

It is well known that both <i>Fagopyrum esculentum</i> species, buckwheat achenes and buckwheat germs, contain flavonoids, and that they can be considered functional foods. In the present study we have analyzed the total content of polyphenols (TPC) and flavonoids (TFC), as well as the a...

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Main Authors: Caterina Dumitru, Rodica Mihaela Dinica, Gabriela-Elena Bahrim, Camelia Vizireanu, Liliana Baroiu, Alina Viorica Iancu, Miruna Draganescu
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/21/10230
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author Caterina Dumitru
Rodica Mihaela Dinica
Gabriela-Elena Bahrim
Camelia Vizireanu
Liliana Baroiu
Alina Viorica Iancu
Miruna Draganescu
author_facet Caterina Dumitru
Rodica Mihaela Dinica
Gabriela-Elena Bahrim
Camelia Vizireanu
Liliana Baroiu
Alina Viorica Iancu
Miruna Draganescu
author_sort Caterina Dumitru
collection DOAJ
description It is well known that both <i>Fagopyrum esculentum</i> species, buckwheat achenes and buckwheat germs, contain flavonoids, and that they can be considered functional foods. In the present study we have analyzed the total content of polyphenols (TPC) and flavonoids (TFC), as well as the antioxidant activity of buckwheat ahcenes originating from the Balti region, Republic of Moldova, and also of the buckwheat sprouts over seven days of germination. The content of phytochemicals in achenes and germinated buckwheat after three and seven days was determined by HPLC–MS analysis. Using the Folin–Ciocalteu method, we recorded an increase in TPC of 360%, and of 436% in TFC after seven days of buckwheat ahcene germination. We aimed to investigate the free radical scavenging properties of methanolic extracts from ahcenes and sprouted buckwheat. We identified and quantified flavonoids and lignans such as rutin, orientin, isoorientin, vitexin, quercetin, quercitrin, isorhamnetin, lutein, apigenin, catechins, coumestrol—which have countless beneficial effects on human health—using HPLC–MS. FTIR analysis also revealed the accumulation of phenolic compounds during germination. This is the first study on the identification and quantification of phytochemicals from buckwheat achenes and sprouts from the Balti region of the Republic of Moldova.
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spelling doaj.art-c84be76df34b4e0e8a786150a4662c9b2023-11-22T20:29:43ZengMDPI AGApplied Sciences2076-34172021-11-0111211023010.3390/app112110230New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of MoldovaCaterina Dumitru0Rodica Mihaela Dinica1Gabriela-Elena Bahrim2Camelia Vizireanu3Liliana Baroiu4Alina Viorica Iancu5Miruna Draganescu6Faculty of Medicine and Pharmacy, “Dunarea de Jos” University from Galati, 47 Domneasca Str., 800008 Galati, RomaniaDepartment of Chemistry, Physics and Environment, “Dunarea de Jos” University from Galati, 111 Domneasca Str., 800201 Galati, RomaniaFaculty of Food Science and Engineering, “Dunarea de Jos” University from Galati, 111 Domneasca Str., 800201 Galati, RomaniaFaculty of Food Science and Engineering, “Dunarea de Jos” University from Galati, 111 Domneasca Str., 800201 Galati, RomaniaFaculty of Medicine and Pharmacy, “Dunarea de Jos” University from Galati, 47 Domneasca Str., 800008 Galati, RomaniaFaculty of Medicine and Pharmacy, “Dunarea de Jos” University from Galati, 47 Domneasca Str., 800008 Galati, RomaniaFaculty of Medicine and Pharmacy, “Dunarea de Jos” University from Galati, 47 Domneasca Str., 800008 Galati, RomaniaIt is well known that both <i>Fagopyrum esculentum</i> species, buckwheat achenes and buckwheat germs, contain flavonoids, and that they can be considered functional foods. In the present study we have analyzed the total content of polyphenols (TPC) and flavonoids (TFC), as well as the antioxidant activity of buckwheat ahcenes originating from the Balti region, Republic of Moldova, and also of the buckwheat sprouts over seven days of germination. The content of phytochemicals in achenes and germinated buckwheat after three and seven days was determined by HPLC–MS analysis. Using the Folin–Ciocalteu method, we recorded an increase in TPC of 360%, and of 436% in TFC after seven days of buckwheat ahcene germination. We aimed to investigate the free radical scavenging properties of methanolic extracts from ahcenes and sprouted buckwheat. We identified and quantified flavonoids and lignans such as rutin, orientin, isoorientin, vitexin, quercetin, quercitrin, isorhamnetin, lutein, apigenin, catechins, coumestrol—which have countless beneficial effects on human health—using HPLC–MS. FTIR analysis also revealed the accumulation of phenolic compounds during germination. This is the first study on the identification and quantification of phytochemicals from buckwheat achenes and sprouts from the Balti region of the Republic of Moldova.https://www.mdpi.com/2076-3417/11/21/10230buckwheat seedsgerminated buckwheatflavonoidslignansrutinvitexin
spellingShingle Caterina Dumitru
Rodica Mihaela Dinica
Gabriela-Elena Bahrim
Camelia Vizireanu
Liliana Baroiu
Alina Viorica Iancu
Miruna Draganescu
New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
Applied Sciences
buckwheat seeds
germinated buckwheat
flavonoids
lignans
rutin
vitexin
title New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
title_full New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
title_fullStr New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
title_full_unstemmed New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
title_short New Insights into the Antioxidant Compounds of Achenes and Sprouted Buckwheat Cultivated in the Republic of Moldova
title_sort new insights into the antioxidant compounds of achenes and sprouted buckwheat cultivated in the republic of moldova
topic buckwheat seeds
germinated buckwheat
flavonoids
lignans
rutin
vitexin
url https://www.mdpi.com/2076-3417/11/21/10230
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