Development of decellularization protocol for caprine small intestine submucosa as a biomaterial
Decellularized animal tissues have been proven to be promising biomaterials for various tissue engineering (TE) applications. Among various animal tissues, small intestine submucosa (SIS) has gained attention of many researchers due to its easy availability from the abattoir waste, excellent physico...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Biomaterials and Biosystems |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666534421000283 |
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| author | Hemant Singh Shiv Dutt Purohit Rakesh Bhaskar Indu Yadav Mukesh Kumar Gupta Narayan Chandra Mishra |
| author_facet | Hemant Singh Shiv Dutt Purohit Rakesh Bhaskar Indu Yadav Mukesh Kumar Gupta Narayan Chandra Mishra |
| author_sort | Hemant Singh |
| collection | DOAJ |
| description | Decellularized animal tissues have been proven to be promising biomaterials for various tissue engineering (TE) applications. Among various animal tissues, small intestine submucosa (SIS) has gained attention of many researchers due to its easy availability from the abattoir waste, excellent physicochemical and biological characteristics of a good biomaterial. In this study, Caprine SIS was decellularized to get decellularized caprine SIS (DG-SIS). For decellularization, several physical, chemical and enzymatic protocols have been described in the literature. To optimize the decellularization of caprine SIS, several decellularization protocol (DP), including an in-house developed by us, had been attempted, and effect of the different DPs on the obtained DG-SIS were assessed in terms of decellularization, physiochemical and biological properties. All the DPs differ in terms of decellularization, but three DPs where ionic detergent like sodium dodecyl sulphate (SDS) has been used, largely affect the native composition (e.g. glycosaminoglycans (GAGs)), biological properties and other physiochemical properties of the G-SIS as compared to the DP that uses hypertonic solution of potassium iodide (KI) and non-ionic detergent (TritonX-100). The obtained DG-SISs were fibrous, hemocompatible, biocompatible, hydrophilic, biodegradable and exhibited significant antibacterial activity. Therefore, the DG-SIS will be a prospective biomaterial for TE applications. |
| first_indexed | 2024-12-24T00:16:14Z |
| format | Article |
| id | doaj.art-c84cab8f8b0743e39670089bca15f593 |
| institution | Directory Open Access Journal |
| issn | 2666-5344 |
| language | English |
| last_indexed | 2024-12-24T00:16:14Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomaterials and Biosystems |
| spelling | doaj.art-c84cab8f8b0743e39670089bca15f5932022-12-21T17:24:45ZengElsevierBiomaterials and Biosystems2666-53442022-03-015100035Development of decellularization protocol for caprine small intestine submucosa as a biomaterialHemant Singh0Shiv Dutt Purohit1Rakesh Bhaskar2Indu Yadav3Mukesh Kumar Gupta4Narayan Chandra Mishra5Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, IndiaDepartment of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, IndiaDepartment of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, Odisha, IndiaDepartment of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, IndiaDepartment of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, Odisha, IndiaDepartment of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India; Corresponding author.Decellularized animal tissues have been proven to be promising biomaterials for various tissue engineering (TE) applications. Among various animal tissues, small intestine submucosa (SIS) has gained attention of many researchers due to its easy availability from the abattoir waste, excellent physicochemical and biological characteristics of a good biomaterial. In this study, Caprine SIS was decellularized to get decellularized caprine SIS (DG-SIS). For decellularization, several physical, chemical and enzymatic protocols have been described in the literature. To optimize the decellularization of caprine SIS, several decellularization protocol (DP), including an in-house developed by us, had been attempted, and effect of the different DPs on the obtained DG-SIS were assessed in terms of decellularization, physiochemical and biological properties. All the DPs differ in terms of decellularization, but three DPs where ionic detergent like sodium dodecyl sulphate (SDS) has been used, largely affect the native composition (e.g. glycosaminoglycans (GAGs)), biological properties and other physiochemical properties of the G-SIS as compared to the DP that uses hypertonic solution of potassium iodide (KI) and non-ionic detergent (TritonX-100). The obtained DG-SISs were fibrous, hemocompatible, biocompatible, hydrophilic, biodegradable and exhibited significant antibacterial activity. Therefore, the DG-SIS will be a prospective biomaterial for TE applications.http://www.sciencedirect.com/science/article/pii/S2666534421000283Caprine small intestine submucosaDecellularization protocolsBiomaterialsTissue engineeringextracellular matrix |
| spellingShingle | Hemant Singh Shiv Dutt Purohit Rakesh Bhaskar Indu Yadav Mukesh Kumar Gupta Narayan Chandra Mishra Development of decellularization protocol for caprine small intestine submucosa as a biomaterial Biomaterials and Biosystems Caprine small intestine submucosa Decellularization protocols Biomaterials Tissue engineering extracellular matrix |
| title | Development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| title_full | Development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| title_fullStr | Development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| title_full_unstemmed | Development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| title_short | Development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| title_sort | development of decellularization protocol for caprine small intestine submucosa as a biomaterial |
| topic | Caprine small intestine submucosa Decellularization protocols Biomaterials Tissue engineering extracellular matrix |
| url | http://www.sciencedirect.com/science/article/pii/S2666534421000283 |
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