New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance
Two mutants with short sepals were identified after ethyl methanesulfonate treatment of Nigella damascena seeds. In one of them (“shs1” gene = short sepal 1), isolated from the line with double flowers, the sepals, in addition to reduced size, were divided into several rounded lobes, which granted...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2023-11-01
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| Series: | Advances in Horticultural Science |
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| Online Access: | https://oaj.fupress.net/index.php/ahs/article/view/14608 |
| _version_ | 1797517890496233472 |
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| author | Viktor Lyakh Anatoly Soroka |
| author_facet | Viktor Lyakh Anatoly Soroka |
| author_sort | Viktor Lyakh |
| collection | DOAJ |
| description |
Two mutants with short sepals were identified after ethyl methanesulfonate treatment of Nigella damascena seeds. In one of them (“shs1” gene = short sepal 1), isolated from the line with double flowers, the sepals, in addition to reduced size, were divided into several rounded lobes, which granted the flower an original rose-like appearance of ornamental value. Another mutant with reduced sepals (“shs2” gene = short sepal 2) was isolated from the line with simple flowers. The allelism test showed that these two genes were non-allelic. Both mutants as pollen parents were crossed with the same line with single flowers. In a dihybrid cross, simple flower, non-reduced sepals (wild type) × double flower, reduced sepals (“shs1” gene) F1 hybrids demonstrated a wild phenotype. F2 progeny, in addition to two parental classes, showed two recombinant classes in a 9:3:3:1 ratio, indicating that flower shape and sepal size were inherited monogenously and independently, and the plant with rose-like flowers was a double recessive homozygote. Reduced sepals (“shs2” gene) in crosses with the single flower line of wild type were inherited as a monogenic recessive trait, showing a 3:1 segregation ratio in F2. Both mutant genes had a number of similar pleiotropic effects, which, however, were different in strength. Thus, both mutant genes shortened leaf segments, divided the cotyledon leaves into several lobes, and caused disturbances in the female generative sphere, leading to a lack of seed setting. At the same time, the identification of mutants as early as at the cotyledon stage, due to the pleiotropic effect, makes it possible to select and maintain them, especially with regard to the mutant with rose-like flowers, which is highly decorative.
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| institution | Directory Open Access Journal |
| issn | 0394-6169 1592-1573 |
| language | English |
| last_indexed | 2024-03-10T07:22:32Z |
| publishDate | 2023-11-01 |
| publisher | Firenze University Press |
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| series | Advances in Horticultural Science |
| spelling | doaj.art-c8503dfa41a948b987a8a1d5f04bf28b2023-11-22T14:30:22ZengFirenze University PressAdvances in Horticultural Science0394-61691592-15732023-11-0137310.36253/ahsc-14608New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritanceViktor Lyakh0Anatoly Soroka1Zaporozhye National University, 66 Zhukovsky Street, 69600 Zaporozhye, Ukraine. Institute of Oilseed Crops, National Academy of Agrarian Sciences of Ukraine, Institutskaya Street 1, 70417 Zaporozhye, Ukraine. Two mutants with short sepals were identified after ethyl methanesulfonate treatment of Nigella damascena seeds. In one of them (“shs1” gene = short sepal 1), isolated from the line with double flowers, the sepals, in addition to reduced size, were divided into several rounded lobes, which granted the flower an original rose-like appearance of ornamental value. Another mutant with reduced sepals (“shs2” gene = short sepal 2) was isolated from the line with simple flowers. The allelism test showed that these two genes were non-allelic. Both mutants as pollen parents were crossed with the same line with single flowers. In a dihybrid cross, simple flower, non-reduced sepals (wild type) × double flower, reduced sepals (“shs1” gene) F1 hybrids demonstrated a wild phenotype. F2 progeny, in addition to two parental classes, showed two recombinant classes in a 9:3:3:1 ratio, indicating that flower shape and sepal size were inherited monogenously and independently, and the plant with rose-like flowers was a double recessive homozygote. Reduced sepals (“shs2” gene) in crosses with the single flower line of wild type were inherited as a monogenic recessive trait, showing a 3:1 segregation ratio in F2. Both mutant genes had a number of similar pleiotropic effects, which, however, were different in strength. Thus, both mutant genes shortened leaf segments, divided the cotyledon leaves into several lobes, and caused disturbances in the female generative sphere, leading to a lack of seed setting. At the same time, the identification of mutants as early as at the cotyledon stage, due to the pleiotropic effect, makes it possible to select and maintain them, especially with regard to the mutant with rose-like flowers, which is highly decorative. https://oaj.fupress.net/index.php/ahs/article/view/14608flower shapeinheritancemutantNigella damascenapleiotropic effectshortened sepal |
| spellingShingle | Viktor Lyakh Anatoly Soroka New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance Advances in Horticultural Science flower shape inheritance mutant Nigella damascena pleiotropic effect shortened sepal |
| title | New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance |
| title_full | New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance |
| title_fullStr | New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance |
| title_full_unstemmed | New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance |
| title_short | New mutations of flower shape in Nigella damascena L., its pleiotropic effects and patterns of inheritance |
| title_sort | new mutations of flower shape in nigella damascena l its pleiotropic effects and patterns of inheritance |
| topic | flower shape inheritance mutant Nigella damascena pleiotropic effect shortened sepal |
| url | https://oaj.fupress.net/index.php/ahs/article/view/14608 |
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