Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes
Introduction/Objective. Thermophilic campylobacters, especially Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), are the most important causes of bacterial diarrhea in developed and developing countries. The disease can occur as a sporadic infection or as large and small outbreaks....
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| Format: | Article |
| Language: | English |
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Serbian Medical Society
2017-01-01
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| Series: | Srpski Arhiv za Celokupno Lekarstvo |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2017/0370-81791700082M.pdf |
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| author | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Ristić Ljiljana Milenković Tatjana Bogdanović Dragan |
| author_facet | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Ristić Ljiljana Milenković Tatjana Bogdanović Dragan |
| author_sort | Miljković-Selimović Biljana |
| collection | DOAJ |
| description | Introduction/Objective. Thermophilic campylobacters, especially Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), are the most important causes of bacterial diarrhea in developed and developing countries. The disease can occur as a sporadic infection or as large and small outbreaks. Phenotyping and genotyping methods are in use to determine similarities between strains as well their possible common origin. The goal of the study was to compare discriminatory power of biotyping tests and comparative genomic fingerprinting (CGF) 40 (100%), as well as a combination of the two tests in detection of clonality or epidemiological relatedness between the studied strains. Methods. We investigated 23 Campylobacter strains using biotyping and CGF typing. Results. We found that biotyping was a more discriminatory method for C. coli, and CGF for C. jejuni strains. In the discrimination of C. jejuni strains, CGF had better discriminatory power [Simpson’s index of diversity (ID) was 0.879] over the discrimination of C. coli strains (Simpson’s ID was 0.389). Conclusion. Biotyping and CGF can be complementary methods in detection of similarity, relatedness and possible common origin between strains since the combination of biotyping and CGF methods gives more precise data about diversity within C. coli and C. jejuni strains. [Project of the Serbian
Ministry of Education, Science and Technological Development, Grant no.
TR34008] |
| first_indexed | 2024-12-20T14:31:05Z |
| format | Article |
| id | doaj.art-c85999f8bdfb4a64803a981f00276e83 |
| institution | Directory Open Access Journal |
| issn | 0370-8179 2406-0895 |
| language | English |
| last_indexed | 2024-12-20T14:31:05Z |
| publishDate | 2017-01-01 |
| publisher | Serbian Medical Society |
| record_format | Article |
| series | Srpski Arhiv za Celokupno Lekarstvo |
| spelling | doaj.art-c85999f8bdfb4a64803a981f00276e832022-12-21T19:37:38ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792406-08952017-01-011459-1049249710.2298/SARH160606082M0370-81791700082MComparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypesMiljković-Selimović Biljana0Babić Tatjana1Kocić Branislava2Ristić Ljiljana3Milenković Tatjana4Bogdanović Dragan5Institute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, Niš + School of Medicine, NišInstitute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, NišInstitute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, Niš + School of Medicine, NišInstitute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, NišInstitute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, NišInstitute for Public Health, Center for Microbiology, National reference laboratory for Campylobacter and Helicobacter, Niš + State University of Novi Pazar, Department of Biomedical Sciences, Novi PazarIntroduction/Objective. Thermophilic campylobacters, especially Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), are the most important causes of bacterial diarrhea in developed and developing countries. The disease can occur as a sporadic infection or as large and small outbreaks. Phenotyping and genotyping methods are in use to determine similarities between strains as well their possible common origin. The goal of the study was to compare discriminatory power of biotyping tests and comparative genomic fingerprinting (CGF) 40 (100%), as well as a combination of the two tests in detection of clonality or epidemiological relatedness between the studied strains. Methods. We investigated 23 Campylobacter strains using biotyping and CGF typing. Results. We found that biotyping was a more discriminatory method for C. coli, and CGF for C. jejuni strains. In the discrimination of C. jejuni strains, CGF had better discriminatory power [Simpson’s index of diversity (ID) was 0.879] over the discrimination of C. coli strains (Simpson’s ID was 0.389). Conclusion. Biotyping and CGF can be complementary methods in detection of similarity, relatedness and possible common origin between strains since the combination of biotyping and CGF methods gives more precise data about diversity within C. coli and C. jejuni strains. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR34008]http://www.doiserbia.nb.rs/img/doi/0370-8179/2017/0370-81791700082M.pdfbiotypingmolecular typingmultiplex PCR |
| spellingShingle | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Ristić Ljiljana Milenković Tatjana Bogdanović Dragan Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes Srpski Arhiv za Celokupno Lekarstvo biotyping molecular typing multiplex PCR |
| title | Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes |
| title_full | Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes |
| title_fullStr | Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes |
| title_full_unstemmed | Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes |
| title_short | Comparative genomic fingerprinting for the subtyping of Campylobacter jejuni and Campylobacter coli biotypes |
| title_sort | comparative genomic fingerprinting for the subtyping of campylobacter jejuni and campylobacter coli biotypes |
| topic | biotyping molecular typing multiplex PCR |
| url | http://www.doiserbia.nb.rs/img/doi/0370-8179/2017/0370-81791700082M.pdf |
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