Impact of template denaturation prior to whole genome amplification on gene detection in high GC-content species, Burkholderia mallei and B. pseudomallei

Impact of template denaturation prior to whole genome amplification on gene detection in high GC-content species, Burkholderia mallei and B. pseudomallei

Abstract Objective In this study, we sought to determine the types and prevalence of antimicrobial resistance determinants (ARDs) in Burkholderia spp. strains using the Antimicrobial Resistance Determinant Microarray (ARDM). Results Whole genome amplicons from 22 B. mallei (BM) and 37 B. pseudomalle...

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Bibliographic Details
Main Authors: Chris R. Taitt, Tomasz A. Leski, Jaimee R. Compton, Amy Chen, Kimberly L. Berk, Robert W. Dorsey, Shanmuga Sozhamannan, Dianne L. Dutt, Gary J. Vora
Format: Article
Language:English
Published: BMC 2024-03-01
Series:BMC Research Notes
Subjects:
Antimicrobial resistance
Resistome
Extended-spectrum β-lactamase
ESBL
Select agents
Online Access:https://doi.org/10.1186/s13104-024-06717-8

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