First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions
Abstract Background The dark septate endophytes (DSE) are endophytic and non-mycorrhizal fungi with the ability to impact and control some plant pathogens and promote plant growth. The aim of this study was to investigate the effectiveness of five different DSE fungal isolates in controlling white r...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-08-01
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| Series: | Egyptian Journal of Biological Pest Control |
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| Online Access: | https://doi.org/10.1186/s41938-023-00725-9 |
| _version_ | 1797451568357834752 |
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| author | Cici Indriani Dalimunthe Surono Bonny Poernomo Wahyu Soekarno Laith Khalil Tawfeeq Al-Ani Abdul Munif Catur Sriherwanto Nicho Nurdebyandaru |
| author_facet | Cici Indriani Dalimunthe Surono Bonny Poernomo Wahyu Soekarno Laith Khalil Tawfeeq Al-Ani Abdul Munif Catur Sriherwanto Nicho Nurdebyandaru |
| author_sort | Cici Indriani Dalimunthe |
| collection | DOAJ |
| description | Abstract Background The dark septate endophytes (DSE) are endophytic and non-mycorrhizal fungi with the ability to impact and control some plant pathogens and promote plant growth. The aim of this study was to investigate the effectiveness of five different DSE fungal isolates in controlling white root rot disease (WRRD) caused by the plant pathogen, Rigidoporus microporus in Hevea brasiliensis in a nursery system. There are no previous reports on the role of DSE in controlling WRRD. In this study, the efficacy of five DSE isolates, including Acrocalymma vagum SBTBMDS 1, Clonostachys chloroleuca TMDS 2.1, Lasiodiplodia theobromae APDS 3.2, Penicillium oxalicum TMDS 3.2, and Fusarium falciforme TBMDS 2.4b, was tested for their ability to reduce the severity of WRRD in vivo. Results The results showed that all the DSE isolates were able to inhibit R. microporus, leading to a decrease in the disease severity of WRRD, with percentages ranging from 7.50 to 17.5% and percentage of disease inhibition from 57.67 to 83.33% than the controls, which had a severity percentage of 45%. The TMDS3.2 isolate showed high efficacy in increasing the girth (137%) and height (63.3%) of H. brasiliensis seedlings, while the isolates of P. oxalicum TMDS 3.2 and F. falciforme TMDS 2.4b enhanced the dry weight (123 and 122%, respectively) than the control. Two isolates of P. oxacilum TMDS 3.2 and C. chloroleuca TMDS 2.1 increased the root volume (120 and 107%, respectively) than the control. Gas chromatography–mass spectrometry (GC–MS) analysis showed the ability of three selected DSE isolates, L. theobromae APDS 3.2, F. falciforme TBMDS 2.4b, and P. oxacilum TMDS 3.2, to produce acetic acid butyl methyl-phosphinoylmethyl ester and ethanone, 1-(4-methyl-1H(imidazol-2-yl)—that previously reported as antimicrobials. Conclusion This study demonstrated that DSE fungal isolates had the potential to act as a biocontrol agent against R. microporus in H. Brasiliensis seedlings. In the future, the findings of this study could be utilized to prevent WRRD, one of the most serious problems in rubber plantations, in an environmentally friendly way by reducing the usage of fungicides. |
| first_indexed | 2024-03-09T14:56:30Z |
| format | Article |
| id | doaj.art-c85d61ea3221493d8ba32363456a976d |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-03-09T14:56:30Z |
| publishDate | 2023-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-c85d61ea3221493d8ba32363456a976d2023-11-26T14:11:41ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-08-0133111210.1186/s41938-023-00725-9First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditionsCici Indriani Dalimunthe0Surono1Bonny Poernomo Wahyu Soekarno2Laith Khalil Tawfeeq Al-Ani3Abdul Munif4Catur Sriherwanto5Nicho Nurdebyandaru6Departement of Plant Protection, Institut Pertanian Bogor (IPB University)Departement of Plant Protection, Institut Pertanian Bogor (IPB University)Departement of Plant Protection, Institut Pertanian Bogor (IPB University)School of Biology Science, Universiti Sains MalaysiaDepartement of Plant Protection, Institut Pertanian Bogor (IPB University)Research Center for Applied Microbiology, Research Organization for Life Sciences and Environment, National Research and Innovation AgencyIndonesian Center for Agricultural Land Resources Standardization (ICALRS)Abstract Background The dark septate endophytes (DSE) are endophytic and non-mycorrhizal fungi with the ability to impact and control some plant pathogens and promote plant growth. The aim of this study was to investigate the effectiveness of five different DSE fungal isolates in controlling white root rot disease (WRRD) caused by the plant pathogen, Rigidoporus microporus in Hevea brasiliensis in a nursery system. There are no previous reports on the role of DSE in controlling WRRD. In this study, the efficacy of five DSE isolates, including Acrocalymma vagum SBTBMDS 1, Clonostachys chloroleuca TMDS 2.1, Lasiodiplodia theobromae APDS 3.2, Penicillium oxalicum TMDS 3.2, and Fusarium falciforme TBMDS 2.4b, was tested for their ability to reduce the severity of WRRD in vivo. Results The results showed that all the DSE isolates were able to inhibit R. microporus, leading to a decrease in the disease severity of WRRD, with percentages ranging from 7.50 to 17.5% and percentage of disease inhibition from 57.67 to 83.33% than the controls, which had a severity percentage of 45%. The TMDS3.2 isolate showed high efficacy in increasing the girth (137%) and height (63.3%) of H. brasiliensis seedlings, while the isolates of P. oxalicum TMDS 3.2 and F. falciforme TMDS 2.4b enhanced the dry weight (123 and 122%, respectively) than the control. Two isolates of P. oxacilum TMDS 3.2 and C. chloroleuca TMDS 2.1 increased the root volume (120 and 107%, respectively) than the control. Gas chromatography–mass spectrometry (GC–MS) analysis showed the ability of three selected DSE isolates, L. theobromae APDS 3.2, F. falciforme TBMDS 2.4b, and P. oxacilum TMDS 3.2, to produce acetic acid butyl methyl-phosphinoylmethyl ester and ethanone, 1-(4-methyl-1H(imidazol-2-yl)—that previously reported as antimicrobials. Conclusion This study demonstrated that DSE fungal isolates had the potential to act as a biocontrol agent against R. microporus in H. Brasiliensis seedlings. In the future, the findings of this study could be utilized to prevent WRRD, one of the most serious problems in rubber plantations, in an environmentally friendly way by reducing the usage of fungicides.https://doi.org/10.1186/s41938-023-00725-9Metabolite compoundsMicrobial biocontrol agentNon-mycorrhizal fungiPlant defenceWhite root rot disease |
| spellingShingle | Cici Indriani Dalimunthe Surono Bonny Poernomo Wahyu Soekarno Laith Khalil Tawfeeq Al-Ani Abdul Munif Catur Sriherwanto Nicho Nurdebyandaru First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions Egyptian Journal of Biological Pest Control Metabolite compounds Microbial biocontrol agent Non-mycorrhizal fungi Plant defence White root rot disease |
| title | First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions |
| title_full | First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions |
| title_fullStr | First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions |
| title_full_unstemmed | First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions |
| title_short | First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions |
| title_sort | first report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on hevea brasiliensis seedlings in nursery conditions |
| topic | Metabolite compounds Microbial biocontrol agent Non-mycorrhizal fungi Plant defence White root rot disease |
| url | https://doi.org/10.1186/s41938-023-00725-9 |
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