Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles
Skeletal muscle, which comprises a significant portion of the body, is responsible for vital functions such as movement, metabolism, and overall health. However, severe injuries often result in volumetric muscle loss (VML) and compromise the regenerative capacity of the muscle. Tissue-engineered mus...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11520 |
| _version_ | 1797589065437020160 |
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| author | Shudong Zhao Jishizhan Chen Lei Wu Xin Tao Naheem Yaqub Jinke Chang |
| author_facet | Shudong Zhao Jishizhan Chen Lei Wu Xin Tao Naheem Yaqub Jinke Chang |
| author_sort | Shudong Zhao |
| collection | DOAJ |
| description | Skeletal muscle, which comprises a significant portion of the body, is responsible for vital functions such as movement, metabolism, and overall health. However, severe injuries often result in volumetric muscle loss (VML) and compromise the regenerative capacity of the muscle. Tissue-engineered muscles offer a potential solution to address lost or damaged muscle tissue, thereby restoring muscle function and improving patients’ quality of life. Induced pluripotent stem cells (iPSCs) have emerged as a valuable cell source for muscle tissue engineering due to their pluripotency and self-renewal capacity, enabling the construction of tissue-engineered artificial skeletal muscles with applications in transplantation, disease modelling, and bio-hybrid robots. Next-generation iPSC-based models have the potential to revolutionize drug discovery by offering personalized muscle cells for testing, reducing reliance on animal models. This review provides a comprehensive overview of iPSCs in tissue-engineered artificial skeletal muscles, highlighting the advancements, applications, advantages, and challenges for clinical translation. We also discussed overcoming limitations and considerations in differentiation protocols, characterization methods, large-scale production, and translational regulations. By tackling these challenges, iPSCs can unlock transformative advancements in muscle tissue engineering and therapeutic interventions for the future. |
| first_indexed | 2024-03-11T01:01:03Z |
| format | Article |
| id | doaj.art-c85dc214c66a4465b7f2b7f563f9fc2b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:01:03Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c85dc214c66a4465b7f2b7f563f9fc2b2023-11-18T19:40:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141152010.3390/ijms241411520Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal MusclesShudong Zhao0Jishizhan Chen1Lei Wu2Xin Tao3Naheem Yaqub4Jinke Chang5Division of Surgery and Interventional Science, University College London, London NW3 2QG, UKDivision of Surgery and Interventional Science, University College London, London NW3 2QG, UKDivision of Surgery and Interventional Science, University College London, London NW3 2QG, UKDepartment of iPS Cell Applications, Kobe University, Kobe 657-8501, JapanDivision of Surgery and Interventional Science, University College London, London NW3 2QG, UKDivision of Surgery and Interventional Science, University College London, London NW3 2QG, UKSkeletal muscle, which comprises a significant portion of the body, is responsible for vital functions such as movement, metabolism, and overall health. However, severe injuries often result in volumetric muscle loss (VML) and compromise the regenerative capacity of the muscle. Tissue-engineered muscles offer a potential solution to address lost or damaged muscle tissue, thereby restoring muscle function and improving patients’ quality of life. Induced pluripotent stem cells (iPSCs) have emerged as a valuable cell source for muscle tissue engineering due to their pluripotency and self-renewal capacity, enabling the construction of tissue-engineered artificial skeletal muscles with applications in transplantation, disease modelling, and bio-hybrid robots. Next-generation iPSC-based models have the potential to revolutionize drug discovery by offering personalized muscle cells for testing, reducing reliance on animal models. This review provides a comprehensive overview of iPSCs in tissue-engineered artificial skeletal muscles, highlighting the advancements, applications, advantages, and challenges for clinical translation. We also discussed overcoming limitations and considerations in differentiation protocols, characterization methods, large-scale production, and translational regulations. By tackling these challenges, iPSCs can unlock transformative advancements in muscle tissue engineering and therapeutic interventions for the future.https://www.mdpi.com/1422-0067/24/14/11520skeletal muscleinduced pluripotent stem cellstissue engineeringtransplantation therapiesdisease modellingbiohybrid muscles |
| spellingShingle | Shudong Zhao Jishizhan Chen Lei Wu Xin Tao Naheem Yaqub Jinke Chang Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles International Journal of Molecular Sciences skeletal muscle induced pluripotent stem cells tissue engineering transplantation therapies disease modelling biohybrid muscles |
| title | Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles |
| title_full | Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles |
| title_fullStr | Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles |
| title_full_unstemmed | Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles |
| title_short | Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles |
| title_sort | induced pluripotent stem cells for tissue engineered skeletal muscles |
| topic | skeletal muscle induced pluripotent stem cells tissue engineering transplantation therapies disease modelling biohybrid muscles |
| url | https://www.mdpi.com/1422-0067/24/14/11520 |
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