Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis
Development of new medicine with fewer deleterious effects and more efficacies for treatment of inflammatory bowel disease is needed. 5-Hydroxytryptamine 3 receptor (5-HT3R) antagonists have exhibited analgesic and anti-inflammatory features in vitro and in vivo. The present study was designed to ev...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=228;epage=236;aulast=Motavallian |
| _version_ | 1819142487019618304 |
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| author | Azadeh Motavallian Mohsen Minaiyan Mohammad Rabbani Parvin Mahzouni Sasan Andalib |
| author_facet | Azadeh Motavallian Mohsen Minaiyan Mohammad Rabbani Parvin Mahzouni Sasan Andalib |
| author_sort | Azadeh Motavallian |
| collection | DOAJ |
| description | Development of new medicine with fewer deleterious effects and more efficacies for treatment of inflammatory bowel disease is needed. 5-Hydroxytryptamine 3 receptor (5-HT3R) antagonists have exhibited analgesic and anti-inflammatory features in vitro and in vivo. The present study was designed to evaluate the anti-inflammatory effect of alosetron, a 5-HT3R antagonist, on trinitrobenzenesulfonic acid (TNBS)-induced ulcerative colitis in rats. Two h subsequent to induce colitis (intracolonic instillation of TNBS, 50 mg/kg) in male Wistar rats, alosetron (1 mg/kg), dexamethasone (1 mg/kg), meta-chlorophenylbiguanide (mCPBG, a 5-HT3R agonist, 5 mg/kg), or alosetron + mCPBG were administrated intraperitoneally for 6 days. Animals were thereafter sacrificed and the efficacy of drugs was evaluated macroscopically, histologically, and biochemically (myeloperoxidase, tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) on distal colon samples. Treatment with alosetron and dexamethasone improved macroscopic and microscopic colonic damages significantly and decreased myeloperoxidase activity and colonic levels of inflammatory cytokines. The profitable effects of alosetron were antagonized by concurrent administration of mCPBG. Our data provided evidence that the protective effects of alosetron on TNBS-induced colitis can be mediated by 5- HT3R. |
| first_indexed | 2024-12-22T12:11:07Z |
| format | Article |
| id | doaj.art-c85fc3ed9a2440d29b59709fea29e7bc |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-22T12:11:07Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-c85fc3ed9a2440d29b59709fea29e7bc2022-12-21T18:26:18ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142019-01-0114322823610.4103/1735-5362.258489Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitisAzadeh MotavallianMohsen MinaiyanMohammad RabbaniParvin MahzouniSasan AndalibDevelopment of new medicine with fewer deleterious effects and more efficacies for treatment of inflammatory bowel disease is needed. 5-Hydroxytryptamine 3 receptor (5-HT3R) antagonists have exhibited analgesic and anti-inflammatory features in vitro and in vivo. The present study was designed to evaluate the anti-inflammatory effect of alosetron, a 5-HT3R antagonist, on trinitrobenzenesulfonic acid (TNBS)-induced ulcerative colitis in rats. Two h subsequent to induce colitis (intracolonic instillation of TNBS, 50 mg/kg) in male Wistar rats, alosetron (1 mg/kg), dexamethasone (1 mg/kg), meta-chlorophenylbiguanide (mCPBG, a 5-HT3R agonist, 5 mg/kg), or alosetron + mCPBG were administrated intraperitoneally for 6 days. Animals were thereafter sacrificed and the efficacy of drugs was evaluated macroscopically, histologically, and biochemically (myeloperoxidase, tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) on distal colon samples. Treatment with alosetron and dexamethasone improved macroscopic and microscopic colonic damages significantly and decreased myeloperoxidase activity and colonic levels of inflammatory cytokines. The profitable effects of alosetron were antagonized by concurrent administration of mCPBG. Our data provided evidence that the protective effects of alosetron on TNBS-induced colitis can be mediated by 5- HT3R.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=228;epage=236;aulast=Motavallianalosetron; inflammatory bowel disease; colitis; 5-ht3 receptor; tnbs. |
| spellingShingle | Azadeh Motavallian Mohsen Minaiyan Mohammad Rabbani Parvin Mahzouni Sasan Andalib Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis Research in Pharmaceutical Sciences alosetron; inflammatory bowel disease; colitis; 5-ht3 receptor; tnbs. |
| title | Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis |
| title_full | Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis |
| title_fullStr | Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis |
| title_full_unstemmed | Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis |
| title_short | Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis |
| title_sort | anti inflammatory effects of alosetron mediated through 5 ht3 receptors on experimental colitis |
| topic | alosetron; inflammatory bowel disease; colitis; 5-ht3 receptor; tnbs. |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=228;epage=236;aulast=Motavallian |
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