Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses
<p>Abstract</p> <p>Background</p> <p>Our previous report that the Norwalk virus nonstructural protein p22 is an antagonist of the cellular secretory pathway suggests a new aspect of norovirus/host interaction. To explore conservation of function of this highly divergent...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-09-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://www.virologyj.com/content/9/1/181 |
| _version_ | 1811328931381379072 |
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| author | Sharp Tyler M Crawford Sue E Ajami Nadim J Neill Frederick H Atmar Robert L Katayama Kazuhiko Utama Budi Estes Mary K |
| author_facet | Sharp Tyler M Crawford Sue E Ajami Nadim J Neill Frederick H Atmar Robert L Katayama Kazuhiko Utama Budi Estes Mary K |
| author_sort | Sharp Tyler M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Our previous report that the Norwalk virus nonstructural protein p22 is an antagonist of the cellular secretory pathway suggests a new aspect of norovirus/host interaction. To explore conservation of function of this highly divergent calicivirus protein, we examined the effects of p22 homologues from four human and two murine noroviruses, and feline calicivirus on the secretory pathway.</p> <p>Findings</p> <p>All human noroviruses examined induced Golgi disruption and inhibited protein secretion, with the genogroup II.4 Houston virus being the most potent antagonist. Genogroup II.6 viruses have a conserved mutation in the mimic of an Endoplasmic Reticulum export signal (MERES) motif that is highly conserved in human norovirus homologues of p22 and is critical for secretory pathway antagonism, and these viruses had reduced levels of Golgi disruption and inhibition of protein secretion. p22 homologues from both persistent and nonpersistent strains of murine norovirus induced Golgi disruption, but only mildly inhibited cellular protein secretion. Feline calicivirus p30 did not induce Golgi disruption or inhibit cellular protein secretion.</p> <p>Conclusions</p> <p>These differences confirm a norovirus-specific effect on host cell secretory pathway antagonism by homologues of p22, which may affect viral replication and/or cellular pathogenesis.</p> |
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| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T15:34:47Z |
| publishDate | 2012-09-01 |
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| record_format | Article |
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| spelling | doaj.art-c865043927e6448d956ad0ed167a25942022-12-22T02:41:18ZengBMCVirology Journal1743-422X2012-09-019118110.1186/1743-422X-9-181Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to norovirusesSharp Tyler MCrawford Sue EAjami Nadim JNeill Frederick HAtmar Robert LKatayama KazuhikoUtama BudiEstes Mary K<p>Abstract</p> <p>Background</p> <p>Our previous report that the Norwalk virus nonstructural protein p22 is an antagonist of the cellular secretory pathway suggests a new aspect of norovirus/host interaction. To explore conservation of function of this highly divergent calicivirus protein, we examined the effects of p22 homologues from four human and two murine noroviruses, and feline calicivirus on the secretory pathway.</p> <p>Findings</p> <p>All human noroviruses examined induced Golgi disruption and inhibited protein secretion, with the genogroup II.4 Houston virus being the most potent antagonist. Genogroup II.6 viruses have a conserved mutation in the mimic of an Endoplasmic Reticulum export signal (MERES) motif that is highly conserved in human norovirus homologues of p22 and is critical for secretory pathway antagonism, and these viruses had reduced levels of Golgi disruption and inhibition of protein secretion. p22 homologues from both persistent and nonpersistent strains of murine norovirus induced Golgi disruption, but only mildly inhibited cellular protein secretion. Feline calicivirus p30 did not induce Golgi disruption or inhibit cellular protein secretion.</p> <p>Conclusions</p> <p>These differences confirm a norovirus-specific effect on host cell secretory pathway antagonism by homologues of p22, which may affect viral replication and/or cellular pathogenesis.</p>http://www.virologyj.com/content/9/1/181CalicivirusNorovirusp22Secretory pathway |
| spellingShingle | Sharp Tyler M Crawford Sue E Ajami Nadim J Neill Frederick H Atmar Robert L Katayama Kazuhiko Utama Budi Estes Mary K Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses Virology Journal Calicivirus Norovirus p22 Secretory pathway |
| title | Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses |
| title_full | Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses |
| title_fullStr | Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses |
| title_full_unstemmed | Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses |
| title_short | Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses |
| title_sort | secretory pathway antagonism by calicivirus homologues of norwalk virus nonstructural protein p22 is restricted to noroviruses |
| topic | Calicivirus Norovirus p22 Secretory pathway |
| url | http://www.virologyj.com/content/9/1/181 |
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