A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films
Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA)...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-06-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/7/452 |
| _version_ | 1818980022366502912 |
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| author | Alexander E. Hampe Zidong Li Sunjay Sethi Pamela J. Lein Erkin Seker |
| author_facet | Alexander E. Hampe Zidong Li Sunjay Sethi Pamela J. Lein Erkin Seker |
| author_sort | Alexander E. Hampe |
| collection | DOAJ |
| description | Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed. |
| first_indexed | 2024-12-20T17:08:49Z |
| format | Article |
| id | doaj.art-c8651433d31a49efb6e79ad730a53a7c |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T17:08:49Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c8651433d31a49efb6e79ad730a53a7c2022-12-21T19:32:11ZengMDPI AGNanomaterials2079-49912018-06-018745210.3390/nano8070452nano8070452A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin FilmsAlexander E. Hampe0Zidong Li1Sunjay Sethi2Pamela J. Lein3Erkin Seker4Department of Biomedical Engineering, University of California—Davis, Davis, CA 95616, USADepartment of Biomedical Engineering, University of California—Davis, Davis, CA 95616, USADepartment of Molecular Biosciences, University of California—Davis, Davis, CA 95616, USADepartment of Molecular Biosciences, University of California—Davis, Davis, CA 95616, USADepartment of Electrical and Computer Engineering, University of California—Davis, Davis, CA 95616, USANanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed.http://www.mdpi.com/2079-4991/8/7/452nanostructurecell-material interactionnanoporous goldadhesion strengthastrocytefocal adhesionmicrofluidic flow-cell |
| spellingShingle | Alexander E. Hampe Zidong Li Sunjay Sethi Pamela J. Lein Erkin Seker A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films Nanomaterials nanostructure cell-material interaction nanoporous gold adhesion strength astrocyte focal adhesion microfluidic flow-cell |
| title | A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films |
| title_full | A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films |
| title_fullStr | A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films |
| title_full_unstemmed | A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films |
| title_short | A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films |
| title_sort | microfluidic platform to study astrocyte adhesion on nanoporous gold thin films |
| topic | nanostructure cell-material interaction nanoporous gold adhesion strength astrocyte focal adhesion microfluidic flow-cell |
| url | http://www.mdpi.com/2079-4991/8/7/452 |
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