Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage.
Environmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in differ...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4440727?pdf=render |
| _version_ | 1818020803671752704 |
|---|---|
| author | Fabio Abeni Francesca Petrera Maurizio Capelletti Aldo Dal Prà Luana Bontempo Agostino Tonon Federica Camin |
| author_facet | Fabio Abeni Francesca Petrera Maurizio Capelletti Aldo Dal Prà Luana Bontempo Agostino Tonon Federica Camin |
| author_sort | Fabio Abeni |
| collection | DOAJ |
| description | Environmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in different body fluids on the basis of different homoeothermic adaptations in varying reproductive stages. Three different reproductive stages (pregnant heifer, primiparous lactating cow, and pluriparous lactating cow) of two dairy cattle breeds (Italian Friesian and Modenese) were studied in winter and summer. Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined. Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk. The effects of the season, reproductive stages and breed on δ2H and δ18O were significant in all the fluids, with few exceptions. Deuterium excess was affected by season in all the analysed fluids. The correlations between water isotope measurements in bovine body fluids ranged between 0.6936 (urine-milk) and 0.7848 (urine-plasma) for δ2H, and between 0.8705 (urine-milk) and 0.9602 (plasma-milk) for δ18O. The increase in both isotopic δ values in all body fluids during summer is representative of a condition in which fractionation took place as a consequence of a different ratio between ingested and excreted water, which leads to an increased presence of the heavy isotopes. The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water. |
| first_indexed | 2024-04-14T08:10:18Z |
| format | Article |
| id | doaj.art-c86732e54d614b49b4812d1e1bad7420 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:10:18Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c86732e54d614b49b4812d1e1bad74202022-12-22T02:04:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012739110.1371/journal.pone.0127391Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage.Fabio AbeniFrancesca PetreraMaurizio CapellettiAldo Dal PràLuana BontempoAgostino TononFederica CaminEnvironmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in different body fluids on the basis of different homoeothermic adaptations in varying reproductive stages. Three different reproductive stages (pregnant heifer, primiparous lactating cow, and pluriparous lactating cow) of two dairy cattle breeds (Italian Friesian and Modenese) were studied in winter and summer. Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined. Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk. The effects of the season, reproductive stages and breed on δ2H and δ18O were significant in all the fluids, with few exceptions. Deuterium excess was affected by season in all the analysed fluids. The correlations between water isotope measurements in bovine body fluids ranged between 0.6936 (urine-milk) and 0.7848 (urine-plasma) for δ2H, and between 0.8705 (urine-milk) and 0.9602 (plasma-milk) for δ18O. The increase in both isotopic δ values in all body fluids during summer is representative of a condition in which fractionation took place as a consequence of a different ratio between ingested and excreted water, which leads to an increased presence of the heavy isotopes. The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.http://europepmc.org/articles/PMC4440727?pdf=render |
| spellingShingle | Fabio Abeni Francesca Petrera Maurizio Capelletti Aldo Dal Prà Luana Bontempo Agostino Tonon Federica Camin Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. PLoS ONE |
| title | Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. |
| title_full | Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. |
| title_fullStr | Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. |
| title_full_unstemmed | Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. |
| title_short | Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage. |
| title_sort | hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season farm breed and reproductive stage |
| url | http://europepmc.org/articles/PMC4440727?pdf=render |
| work_keys_str_mv | AT fabioabeni hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT francescapetrera hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT mauriziocapelletti hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT aldodalpra hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT luanabontempo hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT agostinotonon hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage AT federicacamin hydrogenandoxygenstableisotopefractionationinbodyfluidcompartmentsofdairycattleaccordingtoseasonfarmbreedandreproductivestage |