Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/1/22 |
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| author | Jan Schmidt Nela Pilbauerova Tomas Soukup Tereza Suchankova-Kleplova Jakub Suchanek |
| author_facet | Jan Schmidt Nela Pilbauerova Tomas Soukup Tereza Suchankova-Kleplova Jakub Suchanek |
| author_sort | Jan Schmidt |
| collection | DOAJ |
| description | Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and biodegradability, it is also a suitable scaffold material. However, low molecular weight (LMW) fragments, produced by enzymatic cleavage of HA, have different bioactive properties to high molecular weight (HMW) HA. Thus, the impact of HA must be assessed separately for each molecular weight fraction. In this study, we present the effect of three LMW-HA fragments (800, 1600, and 15,000 Da) on DPSCs in vitro. Discrete biological parameters such as DPSC viability, morphology, and cell surface marker expression were determined. Following treatment with LMW-HA, DPSCs initially presented with an acute reduction in proliferation (<i>p</i> < 0.0016) and soon recovered in subsequent passages. They displayed significant size reduction (<i>p</i> = 0.0078, <i>p</i> = 0.0019, <i>p</i> = 0.0098) while maintaining high expression of DPSC markers (CD29, CD44, CD73, CD90). However, in contrast to controls, a significant phenotypic shift (<i>p</i> < 0.05; CD29, CD34, CD90, CD106, CD117, CD146, CD166) of surface markers was observed. These findings provide a basis for further detailed investigations and present a strong argument for the importance of HA scaffold degradation kinetics analysis. |
| first_indexed | 2024-03-10T13:43:56Z |
| format | Article |
| id | doaj.art-c86c6f5f80764c95b174d8d5c463e56b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:43:56Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c86c6f5f80764c95b174d8d5c463e56b2023-11-21T02:50:39ZengMDPI AGBiomolecules2218-273X2020-12-011112210.3390/biom11010022Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In VitroJan Schmidt0Nela Pilbauerova1Tomas Soukup2Tereza Suchankova-Kleplova3Jakub Suchanek4Department of Dentistry, Charles University, Faculty of Medicine in Hradec Kralove and University Hospital, 500 05 Hradec Kralove, Czech RepublicDepartment of Dentistry, Charles University, Faculty of Medicine in Hradec Kralove and University Hospital, 500 05 Hradec Kralove, Czech RepublicDepartment of Histology and Embryology, Charles University, Faculty of Medicine in Hradec Kralove, 500 03 Hradec Králové, Czech RepublicDepartment of Dentistry, Charles University, Faculty of Medicine in Hradec Kralove and University Hospital, 500 05 Hradec Kralove, Czech RepublicDepartment of Dentistry, Charles University, Faculty of Medicine in Hradec Kralove and University Hospital, 500 05 Hradec Kralove, Czech RepublicHyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and biodegradability, it is also a suitable scaffold material. However, low molecular weight (LMW) fragments, produced by enzymatic cleavage of HA, have different bioactive properties to high molecular weight (HMW) HA. Thus, the impact of HA must be assessed separately for each molecular weight fraction. In this study, we present the effect of three LMW-HA fragments (800, 1600, and 15,000 Da) on DPSCs in vitro. Discrete biological parameters such as DPSC viability, morphology, and cell surface marker expression were determined. Following treatment with LMW-HA, DPSCs initially presented with an acute reduction in proliferation (<i>p</i> < 0.0016) and soon recovered in subsequent passages. They displayed significant size reduction (<i>p</i> = 0.0078, <i>p</i> = 0.0019, <i>p</i> = 0.0098) while maintaining high expression of DPSC markers (CD29, CD44, CD73, CD90). However, in contrast to controls, a significant phenotypic shift (<i>p</i> < 0.05; CD29, CD34, CD90, CD106, CD117, CD146, CD166) of surface markers was observed. These findings provide a basis for further detailed investigations and present a strong argument for the importance of HA scaffold degradation kinetics analysis.https://www.mdpi.com/2218-273X/11/1/22hyaluronic aciddental pulp stem cellslow molecular weight hyaluronic acidtissue engineeringscaffold |
| spellingShingle | Jan Schmidt Nela Pilbauerova Tomas Soukup Tereza Suchankova-Kleplova Jakub Suchanek Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro Biomolecules hyaluronic acid dental pulp stem cells low molecular weight hyaluronic acid tissue engineering scaffold |
| title | Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro |
| title_full | Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro |
| title_fullStr | Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro |
| title_full_unstemmed | Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro |
| title_short | Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro |
| title_sort | low molecular weight hyaluronic acid effect on dental pulp stem cells in vitro |
| topic | hyaluronic acid dental pulp stem cells low molecular weight hyaluronic acid tissue engineering scaffold |
| url | https://www.mdpi.com/2218-273X/11/1/22 |
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