Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice
This research aimed to investigate the immunomodulatory effects of phosphorylated <i>Radix Cyathulae officinalis Kuan</i> polysaccharides (pRCPS) in immunosuppressed mice, improving their cellular and humoral immune function. Our results showed that pRCPS increased serum immunoglobulin (...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/22/4150 |
| _version_ | 1819156054227812352 |
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| author | Haibo Feng Jing Fan Lang Lin Yunjie Liu Dongkun Chai Jie Yang |
| author_facet | Haibo Feng Jing Fan Lang Lin Yunjie Liu Dongkun Chai Jie Yang |
| author_sort | Haibo Feng |
| collection | DOAJ |
| description | This research aimed to investigate the immunomodulatory effects of phosphorylated <i>Radix Cyathulae officinalis Kuan</i> polysaccharides (pRCPS) in immunosuppressed mice, improving their cellular and humoral immune function. Our results showed that pRCPS increased serum immunoglobulin (IgG, IgA, IgM) concentrations significantly, enhanced splenocyte proliferation, and the thymus and spleen indices. pRCPS also promoted phagocytosis in peritoneal macrophages and enhanced cytokine (IFN-γ, IL-2, -4, -5, -6, and -10) serum levels. Importantly, pRCPS increased the proportions of selected T cell subpopulations (CD3<sup>+</sup>, CD4<sup>+</sup>, and the CD4<sup>+</sup> to CD8<sup>+</sup> ratio). Our results revealed that phosphorylation of the polysaccharides promoted their immune-enhancing effects. Thus, pRCPS can enhance cellular and humoral immunity and could be used as an immune-enhancing agent to overcome cyclophosphamide (CY)-induced immunosuppression. |
| first_indexed | 2024-12-22T15:46:46Z |
| format | Article |
| id | doaj.art-c86c8a83ead04b5cb5cb9969b54d115b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T15:46:46Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c86c8a83ead04b5cb5cb9969b54d115b2022-12-21T18:21:01ZengMDPI AGMolecules1420-30492019-11-012422415010.3390/molecules24224150molecules24224150Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed MiceHaibo Feng0Jing Fan1Lang Lin2Yunjie Liu3Dongkun Chai4Jie Yang5College of Life Science and Technology, Southwest Minzu University, Chengdu, Sichuan 6100041, ChinaSichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan 610051, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, ChinaThis research aimed to investigate the immunomodulatory effects of phosphorylated <i>Radix Cyathulae officinalis Kuan</i> polysaccharides (pRCPS) in immunosuppressed mice, improving their cellular and humoral immune function. Our results showed that pRCPS increased serum immunoglobulin (IgG, IgA, IgM) concentrations significantly, enhanced splenocyte proliferation, and the thymus and spleen indices. pRCPS also promoted phagocytosis in peritoneal macrophages and enhanced cytokine (IFN-γ, IL-2, -4, -5, -6, and -10) serum levels. Importantly, pRCPS increased the proportions of selected T cell subpopulations (CD3<sup>+</sup>, CD4<sup>+</sup>, and the CD4<sup>+</sup> to CD8<sup>+</sup> ratio). Our results revealed that phosphorylation of the polysaccharides promoted their immune-enhancing effects. Thus, pRCPS can enhance cellular and humoral immunity and could be used as an immune-enhancing agent to overcome cyclophosphamide (CY)-induced immunosuppression.https://www.mdpi.com/1420-3049/24/22/4150<i>radix cyathula</i> <i>officinalis kuan</i> polysaccharidephosphorylation modificationpolysaccharideimmunosuppressed mice |
| spellingShingle | Haibo Feng Jing Fan Lang Lin Yunjie Liu Dongkun Chai Jie Yang Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice Molecules <i>radix cyathula</i> <i>officinalis kuan</i> polysaccharide phosphorylation modification polysaccharide immunosuppressed mice |
| title | Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice |
| title_full | Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice |
| title_fullStr | Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice |
| title_full_unstemmed | Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice |
| title_short | Immunomodulatory Effects of Phosphorylated <i>Radix Cyathulae officinalis</i> Polysaccharides in Immunosuppressed Mice |
| title_sort | immunomodulatory effects of phosphorylated i radix cyathulae officinalis i polysaccharides in immunosuppressed mice |
| topic | <i>radix cyathula</i> <i>officinalis kuan</i> polysaccharide phosphorylation modification polysaccharide immunosuppressed mice |
| url | https://www.mdpi.com/1420-3049/24/22/4150 |
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