Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma
A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standar...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1211383/full |
| _version_ | 1797736167145209856 |
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| author | Ying Wang Guan-An Zhao Xia Li En Zhang Wei Tan Jia-qi Chen |
| author_facet | Ying Wang Guan-An Zhao Xia Li En Zhang Wei Tan Jia-qi Chen |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standard (IS). The analytes were separated on an Acquity UPLC C18 (2.1 mm × 50 mm, 1.7 μm) chromatographic column with gradient elution using a mobile phase (0.1% formic acid-acetonitrile). Then, the eluates were detected by electrospray ionization (ESI) in positive ion mode. The analytes were quantified by multiple reaction monitoring (MRM) using the transition m/z 303.3→215.0 of safinamide and m/z 285.0→154.0 of IS. Safinamide had good linearity in the concentration range of 1.0–2000 ng/mL, and the lower limit of quantitation (LLOQ) was 1.0 ng/mL. The intra- and inter-day precision and accuracy of safinamide were less than 7.63%, while the average recovery rate was 92.98%–100.29%. The method was validated to be stable and had low noise, short chromatographic run time, wide linear range, small sample volumes, low sample injection volumes, and high sensitivity. Therefore, it can be used in pharmacokinetics and preclinical and clinical studies. |
| first_indexed | 2024-03-12T13:08:45Z |
| format | Article |
| id | doaj.art-c86efbd00fae4ebd9a0dc56ca1f07af3 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-12T13:08:45Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-c86efbd00fae4ebd9a0dc56ca1f07af32023-08-28T08:49:58ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-08-011410.3389/fphar.2023.12113831211383Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasmaYing Wang0Guan-An Zhao1Xia Li2En Zhang3Wei Tan4Jia-qi Chen5Department of Pharmacy, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, ChinaUrinary Surgery, The People’s Hospital of Lishui, Lishui, Zhejiang, ChinaClinical Laboratory, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, ChinaClinical Laboratory, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, ChinaThe Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, ChinaClinical Lab, The People’s Hospital of Lishui, Lishui, Zhejiang, ChinaA fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standard (IS). The analytes were separated on an Acquity UPLC C18 (2.1 mm × 50 mm, 1.7 μm) chromatographic column with gradient elution using a mobile phase (0.1% formic acid-acetonitrile). Then, the eluates were detected by electrospray ionization (ESI) in positive ion mode. The analytes were quantified by multiple reaction monitoring (MRM) using the transition m/z 303.3→215.0 of safinamide and m/z 285.0→154.0 of IS. Safinamide had good linearity in the concentration range of 1.0–2000 ng/mL, and the lower limit of quantitation (LLOQ) was 1.0 ng/mL. The intra- and inter-day precision and accuracy of safinamide were less than 7.63%, while the average recovery rate was 92.98%–100.29%. The method was validated to be stable and had low noise, short chromatographic run time, wide linear range, small sample volumes, low sample injection volumes, and high sensitivity. Therefore, it can be used in pharmacokinetics and preclinical and clinical studies.https://www.frontiersin.org/articles/10.3389/fphar.2023.1211383/fullUPLC-MS/MSratsafinamideplasmapharmacokinetics |
| spellingShingle | Ying Wang Guan-An Zhao Xia Li En Zhang Wei Tan Jia-qi Chen Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma Frontiers in Pharmacology UPLC-MS/MS rat safinamide plasma pharmacokinetics |
| title | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
| title_full | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
| title_fullStr | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
| title_full_unstemmed | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
| title_short | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
| title_sort | establishment of a sensitive uplc ms ms method to quantify safinamide in rat plasma |
| topic | UPLC-MS/MS rat safinamide plasma pharmacokinetics |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1211383/full |
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