Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode
A rapid and simple method for quantitative monitoring of Brucella melitensis using electrochemical impedance spectroscopy (EIS) is reported for the first time. The label-free immunosensors were fabricated by immobilizing Brucella melitensis antibody on the surface of gold nanoparticle-modified scree...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-07-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/7/8551 |
| _version_ | 1811298628050878464 |
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| author | Xiaowen Wang Wei Yang Haili Ma Chuanjin Cui Yueming Zuo Haiyun Wu |
| author_facet | Xiaowen Wang Wei Yang Haili Ma Chuanjin Cui Yueming Zuo Haiyun Wu |
| author_sort | Xiaowen Wang |
| collection | DOAJ |
| description | A rapid and simple method for quantitative monitoring of Brucella melitensis using electrochemical impedance spectroscopy (EIS) is reported for the first time. The label-free immunosensors were fabricated by immobilizing Brucella melitensis antibody on the surface of gold nanoparticle-modified screen-printed carbon electrodes (GNP-SPCEs). Cyclic voltammetry (CV) and EIS were used to characterize the Brucella melitensis antigen interaction on the surface of GNP-SPCEs with antibody. A general electronic equivalent model of an electrochemical cell was introduced for interpretation of the impedance components of the system. The results showed that the change in electron-transfer resistance (Rct) was significantly different due to the binding of Brucella melitensis cells. A linear relationship between the Rct variation and logarithmic value of the cell concentration was found from 4 × 104 to 4 × 106 CFU/mL in pure culture. The label-free impedance biosensor was able to detect as low as 1 × 104 and 4 × 105 CFU/mL of Brucella melitensis in pure culture and milk samples, respectively, in less than 1.5 h. Moreover, a good selectivity versus Escherichia coli O157:H7 and Staphylococcus aureus cells was obtained for our developed immunosensor demonstrating its specificity towards only Brucella melitensis. |
| first_indexed | 2024-04-13T06:22:06Z |
| format | Article |
| id | doaj.art-c86fbdce67784fbd8d8a3cb202edf091 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T06:22:06Z |
| publishDate | 2013-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c86fbdce67784fbd8d8a3cb202edf0912022-12-22T02:58:36ZengMDPI AGSensors1424-82202013-07-011378551856310.3390/s130708551Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon ElectrodeXiaowen WangWei YangHaili MaChuanjin CuiYueming ZuoHaiyun WuA rapid and simple method for quantitative monitoring of Brucella melitensis using electrochemical impedance spectroscopy (EIS) is reported for the first time. The label-free immunosensors were fabricated by immobilizing Brucella melitensis antibody on the surface of gold nanoparticle-modified screen-printed carbon electrodes (GNP-SPCEs). Cyclic voltammetry (CV) and EIS were used to characterize the Brucella melitensis antigen interaction on the surface of GNP-SPCEs with antibody. A general electronic equivalent model of an electrochemical cell was introduced for interpretation of the impedance components of the system. The results showed that the change in electron-transfer resistance (Rct) was significantly different due to the binding of Brucella melitensis cells. A linear relationship between the Rct variation and logarithmic value of the cell concentration was found from 4 × 104 to 4 × 106 CFU/mL in pure culture. The label-free impedance biosensor was able to detect as low as 1 × 104 and 4 × 105 CFU/mL of Brucella melitensis in pure culture and milk samples, respectively, in less than 1.5 h. Moreover, a good selectivity versus Escherichia coli O157:H7 and Staphylococcus aureus cells was obtained for our developed immunosensor demonstrating its specificity towards only Brucella melitensis.http://www.mdpi.com/1424-8220/13/7/8551quantitative detectionBrucella melitensislabel-free immunosensorscreen-printed carbon electrodegold nanoparticle |
| spellingShingle | Xiaowen Wang Wei Yang Haili Ma Chuanjin Cui Yueming Zuo Haiyun Wu Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode Sensors quantitative detection Brucella melitensis label-free immunosensor screen-printed carbon electrode gold nanoparticle |
| title | Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode |
| title_full | Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode |
| title_fullStr | Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode |
| title_full_unstemmed | Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode |
| title_short | Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode |
| title_sort | rapid quantitative detection of brucella melitensis by a label free impedance immunosensor based on a gold nanoparticle modified screen printed carbon electrode |
| topic | quantitative detection Brucella melitensis label-free immunosensor screen-printed carbon electrode gold nanoparticle |
| url | http://www.mdpi.com/1424-8220/13/7/8551 |
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