The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results
The use of protective protein-based nanovehicles to deliver hydrophobic nutraceuticals raises important questions regarding their consequent bioavailability compared to that inside fat, or in its presence. To answer that, vitamin-D3 was chosen as a model lipophilic-bioactive, re-assembled casein mic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-03-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617300191 |
| _version_ | 1818871374022705152 |
|---|---|
| author | Yifat Cohen Sofia Ish-Shalom Elena Segal Olga Nudelman Avi Shpigelman Yoav D. Livney |
| author_facet | Yifat Cohen Sofia Ish-Shalom Elena Segal Olga Nudelman Avi Shpigelman Yoav D. Livney |
| author_sort | Yifat Cohen |
| collection | DOAJ |
| description | The use of protective protein-based nanovehicles to deliver hydrophobic nutraceuticals raises important questions regarding their consequent bioavailability compared to that inside fat, or in its presence. To answer that, vitamin-D3 was chosen as a model lipophilic-bioactive, re-assembled casein micelles (rCM) as a protein-based nanovehicle and 0% fat yoghurt as a model nonfat food. We performed a single-high-dose-ingestion clinical trial with 94 healthy human volunteers, each receiving 150 g yoghurt containing 50,000 IU of vitamin-D3. Vitamin-D3 was either in rCM in the absence or presence of fat, or dissolved in milk fat. Un-enriched nonfat yoghurt was the negative control. The bioavailability of vitamin-D3 was determined by measuring its status-indicator: serum 25-hydroxyvitamin D level. The bioavailability of vitamin-D3 within the protein nanoparticles in the absence of fat was found to be insignificantly different from its bioavailability within fat, suggesting that the bioavailability of lipophilic bioactives in protein nanovehicles is not lower than in fat. |
| first_indexed | 2024-12-19T12:21:54Z |
| format | Article |
| id | doaj.art-c871a39305ef49d1925afe9319e0359b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-19T12:21:54Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c871a39305ef49d1925afe9319e0359b2022-12-21T20:21:45ZengElsevierJournal of Functional Foods1756-46462017-03-0130321325The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial resultsYifat Cohen0Sofia Ish-Shalom1Elena Segal2Olga Nudelman3Avi Shpigelman4Yoav D. Livney5Department of Biotechnology & Food Engineering, Technion- Israel Institute of Technology, Haifa 3200000, IsraelBone and Mineral Metabolism Unit, Rambam Health Care Campus, Haifa 3525408, Israel; Faculty of Medicine, Technion- Israel Institute of Technology, Haifa 3525406, IsraelBone and Mineral Metabolism Unit, Rambam Health Care Campus, Haifa 3525408, Israel; Faculty of Medicine, Technion- Israel Institute of Technology, Haifa 3525406, IsraelBone and Mineral Metabolism Unit, Rambam Health Care Campus, Haifa 3525408, Israel; Faculty of Medicine, Technion- Israel Institute of Technology, Haifa 3525406, IsraelDepartment of Biotechnology & Food Engineering, Technion- Israel Institute of Technology, Haifa 3200000, IsraelDepartment of Biotechnology & Food Engineering, Technion- Israel Institute of Technology, Haifa 3200000, Israel; Russell Berrie Nanotechnology Institute, Technion- Israel Institute of Technology, Haifa 3200000, Israel; Corresponding author at: Department of Biotechnology & Food Engineering, Technion- Israel Institute of Technology, Haifa 3200000, Israel.The use of protective protein-based nanovehicles to deliver hydrophobic nutraceuticals raises important questions regarding their consequent bioavailability compared to that inside fat, or in its presence. To answer that, vitamin-D3 was chosen as a model lipophilic-bioactive, re-assembled casein micelles (rCM) as a protein-based nanovehicle and 0% fat yoghurt as a model nonfat food. We performed a single-high-dose-ingestion clinical trial with 94 healthy human volunteers, each receiving 150 g yoghurt containing 50,000 IU of vitamin-D3. Vitamin-D3 was either in rCM in the absence or presence of fat, or dissolved in milk fat. Un-enriched nonfat yoghurt was the negative control. The bioavailability of vitamin-D3 was determined by measuring its status-indicator: serum 25-hydroxyvitamin D level. The bioavailability of vitamin-D3 within the protein nanoparticles in the absence of fat was found to be insignificantly different from its bioavailability within fat, suggesting that the bioavailability of lipophilic bioactives in protein nanovehicles is not lower than in fat.http://www.sciencedirect.com/science/article/pii/S1756464617300191Vitamin DCasein micellesBioavailabilityClinical studyProtein nanoparticles |
| spellingShingle | Yifat Cohen Sofia Ish-Shalom Elena Segal Olga Nudelman Avi Shpigelman Yoav D. Livney The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results Journal of Functional Foods Vitamin D Casein micelles Bioavailability Clinical study Protein nanoparticles |
| title | The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results |
| title_full | The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results |
| title_fullStr | The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results |
| title_full_unstemmed | The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results |
| title_short | The bioavailability of vitamin D3, a model hydrophobic nutraceutical, in casein micelles, as model protein nanoparticles: Human clinical trial results |
| title_sort | bioavailability of vitamin d3 a model hydrophobic nutraceutical in casein micelles as model protein nanoparticles human clinical trial results |
| topic | Vitamin D Casein micelles Bioavailability Clinical study Protein nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617300191 |
| work_keys_str_mv | AT yifatcohen thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT sofiaishshalom thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT elenasegal thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT olganudelman thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT avishpigelman thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT yoavdlivney thebioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT yifatcohen bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT sofiaishshalom bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT elenasegal bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT olganudelman bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT avishpigelman bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults AT yoavdlivney bioavailabilityofvitamind3amodelhydrophobicnutraceuticalincaseinmicellesasmodelproteinnanoparticleshumanclinicaltrialresults |