Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents
Nanoparticles have gained immense interest as probable drug molecules against microbial infections. Metal nanoparticles synthesized via exploring the reduction potential and capping activity of plants were found to have remarkable antimicrobial activity. The synthesis was conducted without hazardous...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi - SAGE Publishing
2024-01-01
|
| Series: | Nanomaterials and Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2024/9912599 |
| _version_ | 1797356428577472512 |
|---|---|
| author | Priyanka Yadav Monisha Singhal Sreemoyee Chatterjee Surendra Nimesh Nidhi Gupta |
| author_facet | Priyanka Yadav Monisha Singhal Sreemoyee Chatterjee Surendra Nimesh Nidhi Gupta |
| author_sort | Priyanka Yadav |
| collection | DOAJ |
| description | Nanoparticles have gained immense interest as probable drug molecules against microbial infections. Metal nanoparticles synthesized via exploring the reduction potential and capping activity of plants were found to have remarkable antimicrobial activity. The synthesis was conducted without hazardous chemicals and generation of toxic waste products. The focus of the study was, therefore, to investigate the efficacy of silver nanoparticles biosynthesized using Grewia tenax leaf extract as an antibacterial, antibiofilm, and antifungal therapeutic agent. The silver nanoparticles (GTAgNPs) were synthesized using optimized conditions of 2.5 mM AgNO3 and 1 : 10 ratio of 10% extract at 37°C on continuous stirring. The characterization was done by UV-visible spectroscopy, DLS, SEM, zeta potential, and FTIR. The antibacterial activity of GTAgNPs against both Gram (+) Bacillus cereus and Staphylococcus aureus and Gram (−) Escherichia coli and Pseudomonas aeruginosa bacteria via zone of inhibition, MIC, and MBC was analysed. The inhibitory effect of silver nanoparticles on biofilm formation was also observed against these bacteria. These nanoparticles were then evaluated for their potential antifungal activity against Candida albicans and Aspergillus niger by observing fungal growth inhibition. The probable mechanism of antimicrobial activity by GTAgNPs was studied by scanning electron microscopy which showed the significant formation of pores on the cell surface in GTAgNPs-treated microbial cells, leading to the death of the microbial cell. All these studies concluded that GTAgNPs possess the potent antimicrobial potential and can be employed as antimicrobial therapeutic agents. |
| first_indexed | 2024-03-08T14:27:29Z |
| format | Article |
| id | doaj.art-c872e55bad43406885018c1a92b61a48 |
| institution | Directory Open Access Journal |
| issn | 1847-9804 |
| language | English |
| last_indexed | 2024-03-08T14:27:29Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi - SAGE Publishing |
| record_format | Article |
| series | Nanomaterials and Nanotechnology |
| spelling | doaj.art-c872e55bad43406885018c1a92b61a482024-01-13T00:00:04ZengHindawi - SAGE PublishingNanomaterials and Nanotechnology1847-98042024-01-01202410.1155/2024/9912599Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal AgentsPriyanka Yadav0Monisha Singhal1Sreemoyee Chatterjee2Surendra Nimesh3Nidhi Gupta4Department of Microbiology and BiotechnologyDepartment of Microbiology and BiotechnologyDepartment of Microbiology and BiotechnologyDepartment of BiotechnologyDepartment of Microbiology and BiotechnologyNanoparticles have gained immense interest as probable drug molecules against microbial infections. Metal nanoparticles synthesized via exploring the reduction potential and capping activity of plants were found to have remarkable antimicrobial activity. The synthesis was conducted without hazardous chemicals and generation of toxic waste products. The focus of the study was, therefore, to investigate the efficacy of silver nanoparticles biosynthesized using Grewia tenax leaf extract as an antibacterial, antibiofilm, and antifungal therapeutic agent. The silver nanoparticles (GTAgNPs) were synthesized using optimized conditions of 2.5 mM AgNO3 and 1 : 10 ratio of 10% extract at 37°C on continuous stirring. The characterization was done by UV-visible spectroscopy, DLS, SEM, zeta potential, and FTIR. The antibacterial activity of GTAgNPs against both Gram (+) Bacillus cereus and Staphylococcus aureus and Gram (−) Escherichia coli and Pseudomonas aeruginosa bacteria via zone of inhibition, MIC, and MBC was analysed. The inhibitory effect of silver nanoparticles on biofilm formation was also observed against these bacteria. These nanoparticles were then evaluated for their potential antifungal activity against Candida albicans and Aspergillus niger by observing fungal growth inhibition. The probable mechanism of antimicrobial activity by GTAgNPs was studied by scanning electron microscopy which showed the significant formation of pores on the cell surface in GTAgNPs-treated microbial cells, leading to the death of the microbial cell. All these studies concluded that GTAgNPs possess the potent antimicrobial potential and can be employed as antimicrobial therapeutic agents.http://dx.doi.org/10.1155/2024/9912599 |
| spellingShingle | Priyanka Yadav Monisha Singhal Sreemoyee Chatterjee Surendra Nimesh Nidhi Gupta Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents Nanomaterials and Nanotechnology |
| title | Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents |
| title_full | Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents |
| title_fullStr | Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents |
| title_full_unstemmed | Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents |
| title_short | Grewia tenax-Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents |
| title_sort | grewia tenax mediated silver nanoparticles as efficient antibacterial and antifungal agents |
| url | http://dx.doi.org/10.1155/2024/9912599 |
| work_keys_str_mv | AT priyankayadav grewiatenaxmediatedsilvernanoparticlesasefficientantibacterialandantifungalagents AT monishasinghal grewiatenaxmediatedsilvernanoparticlesasefficientantibacterialandantifungalagents AT sreemoyeechatterjee grewiatenaxmediatedsilvernanoparticlesasefficientantibacterialandantifungalagents AT surendranimesh grewiatenaxmediatedsilvernanoparticlesasefficientantibacterialandantifungalagents AT nidhigupta grewiatenaxmediatedsilvernanoparticlesasefficientantibacterialandantifungalagents |