A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae
Abstract The leafhopper genus Arboridia includes several species that feed on Vitis vinifera and cause leaf chlorosis. We report the first alien Arboridia infestation in Italy in 2021 in an Apulian vineyard. To confirm the taxonomic status of the species responsible for crop damage, and reconstruct...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49410-9 |
| _version_ | 1797276634858913792 |
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| author | Riccardo Piccinno Alessia Tatti Sabina Avosani Giulio Galla Valentina Lazazzara Federico Pedrazzoli Nicola Zadra Mirco Rodeghiero Gabrijel Seljak İnanç Özgen Heidi C. Hauffe Vincenzo Verrastro Marco Valerio Rossi Stacconi Valerio Mazzoni Omar Rota-Stabelli |
| author_facet | Riccardo Piccinno Alessia Tatti Sabina Avosani Giulio Galla Valentina Lazazzara Federico Pedrazzoli Nicola Zadra Mirco Rodeghiero Gabrijel Seljak İnanç Özgen Heidi C. Hauffe Vincenzo Verrastro Marco Valerio Rossi Stacconi Valerio Mazzoni Omar Rota-Stabelli |
| author_sort | Riccardo Piccinno |
| collection | DOAJ |
| description | Abstract The leafhopper genus Arboridia includes several species that feed on Vitis vinifera and cause leaf chlorosis. We report the first alien Arboridia infestation in Italy in 2021 in an Apulian vineyard. To confirm the taxonomic status of the species responsible for crop damage, and reconstruct its demographic history, we barcoded individuals from Apulia together with Arboridia spp. from Crete (Greece), A. adanae from Central Turkey and other specimens of the presumed sister species, A. dalmatina from Dalmatia (Croatia). Molecular phylogenies and barcoding gap analysis identified clades not associated with sampling locations. This result is incongruent with classical specimen assignment and is further supported by morphological analyses, which did not reveal significant differences among the populations. Therefore, we propose A. dalmatina as a junior synonym of A. adanae, which would become the only grapevine-related Arboridia species in the eastern Mediterranean. To further characterise A. adanae evolution, we performed a molecular clock analysis that suggested a radiation during the Pleistocene glaciations. Finally, to assess whether the Apulian individuals carried microorganisms of agricultural relevance, we sequenced their bacterial microbiota using 16S rRNA amplicon sequencing identifying three phytopathogens not generally associated with Arboridia activities as well as Wolbachia in one Apulian haplogroup. We discuss the agricultural implications of this infestation. |
| first_indexed | 2024-03-07T15:31:03Z |
| format | Article |
| id | doaj.art-c876b4bad09844328a8badc1039a41d6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:31:03Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c876b4bad09844328a8badc1039a41d62024-03-05T16:26:48ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-023-49410-9A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanaeRiccardo Piccinno0Alessia Tatti1Sabina Avosani2Giulio Galla3Valentina Lazazzara4Federico Pedrazzoli5Nicola Zadra6Mirco Rodeghiero7Gabrijel Seljakİnanç Özgen8Heidi C. Hauffe9Vincenzo Verrastro10Marco Valerio Rossi Stacconi11Valerio Mazzoni12Omar Rota-Stabelli13Center Agriculture Food Environment (C3A), University of Trento, San Michele All’AdigeCenter Agriculture Food Environment (C3A), University of Trento, San Michele All’AdigeFaculty of Science and Medicine, University of FribourgConservation Genomics Research Unit, Research and Innovation Centre, Fondazione Edmund Mach, San Michele All’AdigeInstitute for Sustainable Plant Protection, National Research Council of ItalyPlant Protection Unit, Research and Innovation Centre, San Michele All’AdigeConservation Genomics Research Unit, Research and Innovation Centre, Fondazione Edmund Mach, San Michele All’AdigeCenter Agriculture Food Environment (C3A), University of Trento, San Michele All’AdigeBioengineering Department, Engineering Faculty, Fırat UniversityConservation Genomics Research Unit, Research and Innovation Centre, Fondazione Edmund Mach, San Michele All’AdigeInternational Centre for Advanced Mediterranean Agronomic Studies (CIHEAM) - BariPlant Protection Unit, Research and Innovation Centre, San Michele All’AdigePlant Protection Unit, Research and Innovation Centre, San Michele All’AdigeCenter Agriculture Food Environment (C3A), University of Trento, San Michele All’AdigeAbstract The leafhopper genus Arboridia includes several species that feed on Vitis vinifera and cause leaf chlorosis. We report the first alien Arboridia infestation in Italy in 2021 in an Apulian vineyard. To confirm the taxonomic status of the species responsible for crop damage, and reconstruct its demographic history, we barcoded individuals from Apulia together with Arboridia spp. from Crete (Greece), A. adanae from Central Turkey and other specimens of the presumed sister species, A. dalmatina from Dalmatia (Croatia). Molecular phylogenies and barcoding gap analysis identified clades not associated with sampling locations. This result is incongruent with classical specimen assignment and is further supported by morphological analyses, which did not reveal significant differences among the populations. Therefore, we propose A. dalmatina as a junior synonym of A. adanae, which would become the only grapevine-related Arboridia species in the eastern Mediterranean. To further characterise A. adanae evolution, we performed a molecular clock analysis that suggested a radiation during the Pleistocene glaciations. Finally, to assess whether the Apulian individuals carried microorganisms of agricultural relevance, we sequenced their bacterial microbiota using 16S rRNA amplicon sequencing identifying three phytopathogens not generally associated with Arboridia activities as well as Wolbachia in one Apulian haplogroup. We discuss the agricultural implications of this infestation.https://doi.org/10.1038/s41598-023-49410-9 |
| spellingShingle | Riccardo Piccinno Alessia Tatti Sabina Avosani Giulio Galla Valentina Lazazzara Federico Pedrazzoli Nicola Zadra Mirco Rodeghiero Gabrijel Seljak İnanç Özgen Heidi C. Hauffe Vincenzo Verrastro Marco Valerio Rossi Stacconi Valerio Mazzoni Omar Rota-Stabelli A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae Scientific Reports |
| title | A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae |
| title_full | A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae |
| title_fullStr | A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae |
| title_full_unstemmed | A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae |
| title_short | A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae |
| title_sort | multidisciplinary approach to tackling invasive species barcoding morphology and metataxonomy of the leafhopper arboridia adanae |
| url | https://doi.org/10.1038/s41598-023-49410-9 |
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