Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes
Medicinal plants have been used for many years by communities to treat illnesses. The need for scientific proof of these vegetable’s curative effects is as necessary as the proof of the inexistence of toxicity related to the use of extracts with therapeutic potential. Annona squamosa L. (Annonaceae)...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Toxicology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666027X23000014 |
| _version_ | 1797798628659560448 |
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| author | Débora Odília Duarte Leite Cicera Janaíne Camilo José Walber Gonçalves Castro Alexandro Rodrigues Dantas Fabíola Fernandes Galvão Rodrigues Pablo Antonio Maia de Farias Edlane Martins de Andrade Henrique Douglas Melo Coutinho José Galberto Martins da Costa |
| author_facet | Débora Odília Duarte Leite Cicera Janaíne Camilo José Walber Gonçalves Castro Alexandro Rodrigues Dantas Fabíola Fernandes Galvão Rodrigues Pablo Antonio Maia de Farias Edlane Martins de Andrade Henrique Douglas Melo Coutinho José Galberto Martins da Costa |
| author_sort | Débora Odília Duarte Leite |
| collection | DOAJ |
| description | Medicinal plants have been used for many years by communities to treat illnesses. The need for scientific proof of these vegetable’s curative effects is as necessary as the proof of the inexistence of toxicity related to the use of extracts with therapeutic potential. Annona squamosa L. (Annonaceae), popularly known as “pinha”, “ata” or “fruta do conde”, has been used in traditional medicine for its analgesic and antitumor activities. The toxic effects attributed to this plant have also been explored as a pesticide and an insecticide. The aim of the present study was to investigate the toxicity of the methanolic extract of A. squamosa seeds and pulp against human erythrocytes. Blood samples were treated with methanolic extract at different concentrations, osmotic fragility was determined using saline tension assays and morphological analyzes were performed using optical microscopy. The extracts were analyzed using high performance liquid chromatography with diode array detection (HPLC-DAD) for phenolic quantification. The seed’s methanolic extract showed toxicity above 50% from a concentration of 100 µg/mL, while also presenting echinocytes in the morphological analysis. The pulp’s methanolic extract did not show toxicity to red blood cells or morphological changes at the concentrations tested. HPLC-DAD analysis revealed the presence of caffeic acid in the seed extract and gallic acid in the pulp extract. The seed’s methanolic extract is toxic and the pulp’s methanolic extract showed no toxicity against human erythrocytes. |
| first_indexed | 2024-03-13T04:07:43Z |
| format | Article |
| id | doaj.art-c87a10dd3005414ab453f856eaa4b20f |
| institution | Directory Open Access Journal |
| issn | 2666-027X |
| language | English |
| last_indexed | 2024-03-13T04:07:43Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Toxicology |
| spelling | doaj.art-c87a10dd3005414ab453f856eaa4b20f2023-06-21T06:59:59ZengElsevierCurrent Research in Toxicology2666-027X2023-01-014100103Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytesDébora Odília Duarte Leite0Cicera Janaíne Camilo1José Walber Gonçalves Castro2Alexandro Rodrigues Dantas3Fabíola Fernandes Galvão Rodrigues4Pablo Antonio Maia de Farias5Edlane Martins de Andrade6Henrique Douglas Melo Coutinho7José Galberto Martins da Costa8Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Ceará, Fortaleza 60740-000, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato 63105-000, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato 63105-000, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato 63105-000, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato 63105-000, BrazilCECAPE College. Av. Padre Cícero, 3917 – São José, Juazeiro do Norte – CE 63024-015, BrazilCECAPE College. Av. Padre Cícero, 3917 – São José, Juazeiro do Norte – CE 63024-015, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil; Corresponding authors at: Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Ceará, Fortaleza 60740-000, Brazil (J.G.M. da Costa).Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Ceará, Fortaleza 60740-000, Brazil; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil; Corresponding authors at: Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Ceará, Fortaleza 60740-000, Brazil (J.G.M. da Costa).Medicinal plants have been used for many years by communities to treat illnesses. The need for scientific proof of these vegetable’s curative effects is as necessary as the proof of the inexistence of toxicity related to the use of extracts with therapeutic potential. Annona squamosa L. (Annonaceae), popularly known as “pinha”, “ata” or “fruta do conde”, has been used in traditional medicine for its analgesic and antitumor activities. The toxic effects attributed to this plant have also been explored as a pesticide and an insecticide. The aim of the present study was to investigate the toxicity of the methanolic extract of A. squamosa seeds and pulp against human erythrocytes. Blood samples were treated with methanolic extract at different concentrations, osmotic fragility was determined using saline tension assays and morphological analyzes were performed using optical microscopy. The extracts were analyzed using high performance liquid chromatography with diode array detection (HPLC-DAD) for phenolic quantification. The seed’s methanolic extract showed toxicity above 50% from a concentration of 100 µg/mL, while also presenting echinocytes in the morphological analysis. The pulp’s methanolic extract did not show toxicity to red blood cells or morphological changes at the concentrations tested. HPLC-DAD analysis revealed the presence of caffeic acid in the seed extract and gallic acid in the pulp extract. The seed’s methanolic extract is toxic and the pulp’s methanolic extract showed no toxicity against human erythrocytes.http://www.sciencedirect.com/science/article/pii/S2666027X23000014ErythrocytesCytotoxicityAnnona squamosa |
| spellingShingle | Débora Odília Duarte Leite Cicera Janaíne Camilo José Walber Gonçalves Castro Alexandro Rodrigues Dantas Fabíola Fernandes Galvão Rodrigues Pablo Antonio Maia de Farias Edlane Martins de Andrade Henrique Douglas Melo Coutinho José Galberto Martins da Costa Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes Current Research in Toxicology Erythrocytes Cytotoxicity Annona squamosa |
| title | Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes |
| title_full | Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes |
| title_fullStr | Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes |
| title_full_unstemmed | Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes |
| title_short | Influence of methanolic extracts from seeds and pulp of Annona squamosa L. on osmotic and morphological fragility in human erythrocytes |
| title_sort | influence of methanolic extracts from seeds and pulp of annona squamosa l on osmotic and morphological fragility in human erythrocytes |
| topic | Erythrocytes Cytotoxicity Annona squamosa |
| url | http://www.sciencedirect.com/science/article/pii/S2666027X23000014 |
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