Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization
Abstract Background Agar is used as a gelling agent that possesses a variety of biological properties; it consists of the polysaccharides agarose and porphyrin. In addition, the monomeric sugars generated after agar hydrolysis can be functionalized for use in biorefineries and biofuel production. Th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02441-w |
| _version_ | 1797388502856368128 |
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| author | Anoth Maharjan Wonho Choi Hee Taek Kim Jung-Ho Park |
| author_facet | Anoth Maharjan Wonho Choi Hee Taek Kim Jung-Ho Park |
| author_sort | Anoth Maharjan |
| collection | DOAJ |
| description | Abstract Background Agar is used as a gelling agent that possesses a variety of biological properties; it consists of the polysaccharides agarose and porphyrin. In addition, the monomeric sugars generated after agar hydrolysis can be functionalized for use in biorefineries and biofuel production. The main objective of this study was to develop a sustainable agar hydrolysis process for bioethanol production using nanotechnology. Peroxidase-mimicking Fe3O4-MNPs were applied for agar degradation to generate agar hydrolysate-soluble fractions amenable to Saccharomyces cerevisiae and Escherichia coli during fermentation. Results Fe3O4-MNP-treated (Fe3O4-MNPs, 1 g/L) agar exhibited 0.903 g/L of reducing sugar, which was 21-fold higher than that of the control (without Fe3O4-MNP-treated). Approximately 0.0181% and 0.0042% of ethanol from 1% of agar was achieved using Saccharomyces cerevisiae and Escherichia coli, respectively, after process optimization. Furthermore, different analytical techniques (FTIR, SEM, TEM, EDS, XRD, and TGA) were applied to validate the efficiency of Fe3O4-MNPs in agar degradation. Conclusions To the best of our knowledge, Fe3O4-MNP-treated agar degradation for bioethanol production through process optimization is a simpler, easier, and novel method for commercialization. |
| first_indexed | 2024-03-08T22:41:45Z |
| format | Article |
| id | doaj.art-c87d9659b7e342ec9b9a569523ccb47b |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-08T22:41:45Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-c87d9659b7e342ec9b9a569523ccb47b2023-12-17T12:09:44ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-12-0116111710.1186/s13068-023-02441-wCatalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterizationAnoth Maharjan0Wonho Choi1Hee Taek Kim2Jung-Ho Park3Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology4D Convergence Technology Institute (National Key Technology Institute in University), Korea National University of TransportationDepartment of Food Science and Technology, Chungnam National UniversityBio-Evaluation Center, Korea Research Institute of Bioscience and BiotechnologyAbstract Background Agar is used as a gelling agent that possesses a variety of biological properties; it consists of the polysaccharides agarose and porphyrin. In addition, the monomeric sugars generated after agar hydrolysis can be functionalized for use in biorefineries and biofuel production. The main objective of this study was to develop a sustainable agar hydrolysis process for bioethanol production using nanotechnology. Peroxidase-mimicking Fe3O4-MNPs were applied for agar degradation to generate agar hydrolysate-soluble fractions amenable to Saccharomyces cerevisiae and Escherichia coli during fermentation. Results Fe3O4-MNP-treated (Fe3O4-MNPs, 1 g/L) agar exhibited 0.903 g/L of reducing sugar, which was 21-fold higher than that of the control (without Fe3O4-MNP-treated). Approximately 0.0181% and 0.0042% of ethanol from 1% of agar was achieved using Saccharomyces cerevisiae and Escherichia coli, respectively, after process optimization. Furthermore, different analytical techniques (FTIR, SEM, TEM, EDS, XRD, and TGA) were applied to validate the efficiency of Fe3O4-MNPs in agar degradation. Conclusions To the best of our knowledge, Fe3O4-MNP-treated agar degradation for bioethanol production through process optimization is a simpler, easier, and novel method for commercialization.https://doi.org/10.1186/s13068-023-02441-wMagnetic nanoparticlesOptimizationHydrolysisAgarBioethanol |
| spellingShingle | Anoth Maharjan Wonho Choi Hee Taek Kim Jung-Ho Park Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization Biotechnology for Biofuels and Bioproducts Magnetic nanoparticles Optimization Hydrolysis Agar Bioethanol |
| title | Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization |
| title_full | Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization |
| title_fullStr | Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization |
| title_full_unstemmed | Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization |
| title_short | Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization |
| title_sort | catalytic hydrolysis of agar using magnetic nanoparticles optimization and characterization |
| topic | Magnetic nanoparticles Optimization Hydrolysis Agar Bioethanol |
| url | https://doi.org/10.1186/s13068-023-02441-w |
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