Cas9 targeted enrichment of mobile elements using nanopore sequencing
Mobile element insertions (MEIs) are a source of repetitive genetic variation and can lead to genetic disorders. Here the authors use Cas9-targeted nanopore sequencing to efficiently saturate enrichment for known and non-reference MEIs.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-23918-y |
| _version_ | 1818722482814713856 |
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| author | Torrin L. McDonald Weichen Zhou Christopher P. Castro Camille Mumm Jessica A. Switzenberg Ryan E. Mills Alan P. Boyle |
| author_facet | Torrin L. McDonald Weichen Zhou Christopher P. Castro Camille Mumm Jessica A. Switzenberg Ryan E. Mills Alan P. Boyle |
| author_sort | Torrin L. McDonald |
| collection | DOAJ |
| description | Mobile element insertions (MEIs) are a source of repetitive genetic variation and can lead to genetic disorders. Here the authors use Cas9-targeted nanopore sequencing to efficiently saturate enrichment for known and non-reference MEIs. |
| first_indexed | 2024-12-17T20:55:20Z |
| format | Article |
| id | doaj.art-c87dab68727b4dfeabb3b16502eb685a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T20:55:20Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c87dab68727b4dfeabb3b16502eb685a2022-12-21T21:32:54ZengNature PortfolioNature Communications2041-17232021-06-0112111310.1038/s41467-021-23918-yCas9 targeted enrichment of mobile elements using nanopore sequencingTorrin L. McDonald0Weichen Zhou1Christopher P. Castro2Camille Mumm3Jessica A. Switzenberg4Ryan E. Mills5Alan P. Boyle6Department of Human Genetics, University of MichiganDepartment of Computational Medicine and Bioinformatics, University of MichiganDepartment of Computational Medicine and Bioinformatics, University of MichiganDepartment of Human Genetics, University of MichiganDepartment of Computational Medicine and Bioinformatics, University of MichiganDepartment of Human Genetics, University of MichiganDepartment of Human Genetics, University of MichiganMobile element insertions (MEIs) are a source of repetitive genetic variation and can lead to genetic disorders. Here the authors use Cas9-targeted nanopore sequencing to efficiently saturate enrichment for known and non-reference MEIs.https://doi.org/10.1038/s41467-021-23918-y |
| spellingShingle | Torrin L. McDonald Weichen Zhou Christopher P. Castro Camille Mumm Jessica A. Switzenberg Ryan E. Mills Alan P. Boyle Cas9 targeted enrichment of mobile elements using nanopore sequencing Nature Communications |
| title | Cas9 targeted enrichment of mobile elements using nanopore sequencing |
| title_full | Cas9 targeted enrichment of mobile elements using nanopore sequencing |
| title_fullStr | Cas9 targeted enrichment of mobile elements using nanopore sequencing |
| title_full_unstemmed | Cas9 targeted enrichment of mobile elements using nanopore sequencing |
| title_short | Cas9 targeted enrichment of mobile elements using nanopore sequencing |
| title_sort | cas9 targeted enrichment of mobile elements using nanopore sequencing |
| url | https://doi.org/10.1038/s41467-021-23918-y |
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