The changes of gut microbiota after acute myocardial infarction in rats.
Recent studies suggested that gut microbiota was involved in the development of coronary artery disease. However, the changes of gut microbiota following acute myocardial infarction (AMI) remain unknown. In this study, a total of 66 male Wistar rats were randomly divided into control, AMI and SHAM g...
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| Format: | Article |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5501596?pdf=render |
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| author | Ze-Xuan Wu Su-Fang Li Hong Chen Jun-Xian Song Yuan-Feng Gao Feng Zhang Cheng-Fu Cao |
| author_facet | Ze-Xuan Wu Su-Fang Li Hong Chen Jun-Xian Song Yuan-Feng Gao Feng Zhang Cheng-Fu Cao |
| author_sort | Ze-Xuan Wu |
| collection | DOAJ |
| description | Recent studies suggested that gut microbiota was involved in the development of coronary artery disease. However, the changes of gut microbiota following acute myocardial infarction (AMI) remain unknown. In this study, a total of 66 male Wistar rats were randomly divided into control, AMI and SHAM groups. The controls (n = 6) were sacrificed after anesthesia. The AMI model was built by ligation of left anterior descending coronary artery. The rats of AMI and SHAM groups were sacrificed at 12 h, 1 d, 3 d, 7 d and 14 d post-operation respectively. Gut microbiota was analyzed by 16S rDNA high throughput sequencing. The gut barrier injuries were evaluated through histopathology, transmission electron microscope and immunohistochemical staining. The richness of gut microbiota was significantly higher in AMI group than SHAM group at 7 d after AMI (P<0.05). Principal coordinate analysis with unweighted UniFrac distances revealed microbial differences between AMI and SHAM groups at 7 d. The gut barrier impairment was also the most significant at 7 d post-AMI. We further identified the differences of microorganisms between AMI and SHAM group at 7 d. The abundance of Synergistetes phylum, Spirochaetes phylum, Lachnospiraceae family, Syntrophomonadaceae family and Tissierella Soehngenia genus was higher in AMI group compared with SHAM group at 7 d post-operation (q<0.05). Our study showed the changes of gut microbiota at day 7 post AMI which was paralleled with intestinal barrier impairment. We also identified the microbial organisms that contribute most. |
| first_indexed | 2024-12-13T21:37:51Z |
| format | Article |
| id | doaj.art-c87db724f0544cc1b00759541645a000 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:37:51Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-c87db724f0544cc1b00759541645a0002022-12-21T23:30:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018071710.1371/journal.pone.0180717The changes of gut microbiota after acute myocardial infarction in rats.Ze-Xuan WuSu-Fang LiHong ChenJun-Xian SongYuan-Feng GaoFeng ZhangCheng-Fu CaoRecent studies suggested that gut microbiota was involved in the development of coronary artery disease. However, the changes of gut microbiota following acute myocardial infarction (AMI) remain unknown. In this study, a total of 66 male Wistar rats were randomly divided into control, AMI and SHAM groups. The controls (n = 6) were sacrificed after anesthesia. The AMI model was built by ligation of left anterior descending coronary artery. The rats of AMI and SHAM groups were sacrificed at 12 h, 1 d, 3 d, 7 d and 14 d post-operation respectively. Gut microbiota was analyzed by 16S rDNA high throughput sequencing. The gut barrier injuries were evaluated through histopathology, transmission electron microscope and immunohistochemical staining. The richness of gut microbiota was significantly higher in AMI group than SHAM group at 7 d after AMI (P<0.05). Principal coordinate analysis with unweighted UniFrac distances revealed microbial differences between AMI and SHAM groups at 7 d. The gut barrier impairment was also the most significant at 7 d post-AMI. We further identified the differences of microorganisms between AMI and SHAM group at 7 d. The abundance of Synergistetes phylum, Spirochaetes phylum, Lachnospiraceae family, Syntrophomonadaceae family and Tissierella Soehngenia genus was higher in AMI group compared with SHAM group at 7 d post-operation (q<0.05). Our study showed the changes of gut microbiota at day 7 post AMI which was paralleled with intestinal barrier impairment. We also identified the microbial organisms that contribute most.http://europepmc.org/articles/PMC5501596?pdf=render |
| spellingShingle | Ze-Xuan Wu Su-Fang Li Hong Chen Jun-Xian Song Yuan-Feng Gao Feng Zhang Cheng-Fu Cao The changes of gut microbiota after acute myocardial infarction in rats. PLoS ONE |
| title | The changes of gut microbiota after acute myocardial infarction in rats. |
| title_full | The changes of gut microbiota after acute myocardial infarction in rats. |
| title_fullStr | The changes of gut microbiota after acute myocardial infarction in rats. |
| title_full_unstemmed | The changes of gut microbiota after acute myocardial infarction in rats. |
| title_short | The changes of gut microbiota after acute myocardial infarction in rats. |
| title_sort | changes of gut microbiota after acute myocardial infarction in rats |
| url | http://europepmc.org/articles/PMC5501596?pdf=render |
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