Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production
We report here the enhanced hemicellulase production by a Talaromyces thermophilus strain in a fed-batch fermentation using 3.6-litre laboratory-controlled bioreactor. When grown on wheat bran, this fungus produces a wide spectrum of polysaccharide-hydrolysing enzymes, mainly endo-β-1,4-xylanase (27...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2013-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/169426 |
| _version_ | 1797421420976799744 |
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| author | Ines Maalej-Achouri Hafedh Belghith Mohamed Guerfali |
| author_facet | Ines Maalej-Achouri Hafedh Belghith Mohamed Guerfali |
| author_sort | Ines Maalej-Achouri |
| collection | DOAJ |
| description | We report here the enhanced hemicellulase production by a Talaromyces thermophilus strain in a fed-batch fermentation using 3.6-litre laboratory-controlled bioreactor. When grown on wheat bran, this fungus produces a wide spectrum of polysaccharide-hydrolysing enzymes, mainly endo-β-1,4-xylanase (27 U/mL), β-xylosidase (1.4 U/mL), α-L-arabinofuranosidase (1.05 U/mL) and β-D-mannosidase (0.78 U/mL). The β-xylosidase was purified and shown to hydrolyse xylobiose and short xylooligosaccharides, but it was inactive on xylan. It released xylose from xylooligosaccharides with a degree of polymerisation ranging from 2 to 5. Talaromyces thermophilus β-xylosidase activity was unaffected by high glucose or arabinose concentration (0.5 M) and retained 75 % of its original activity in the presence of 133 mM xylose. Chitosan-immobilised β-xylosidase was used in a continuous process of conversion of wheat bran hydrolysate to xylose in a packed bed reactor. Xylose production of 18.6 mg/g was reached after six hours in the bioreactor and was twofold higher than that produced by the free enzyme. The produced xylose was further converted into xylitol using the crude intracellular enzyme of Talaromyces thermophilus. |
| first_indexed | 2024-03-09T07:15:55Z |
| format | Article |
| id | doaj.art-c87ec6c3daf04462bbc64d128b5ae9a0 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:15:55Z |
| publishDate | 2013-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-c87ec6c3daf04462bbc64d128b5ae9a02023-12-03T08:31:16ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062013-01-01514479487Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose ProductionInes Maalej-Achouri0Hafedh Belghith1Mohamed Guerfali2Laboratory of Biomass Valorisation and Protein Production in Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, TN-3038 Sfax, TunisiaLaboratory of Biomass Valorisation and Protein Production in Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, TN-3038 Sfax, TunisiaLaboratory of Biomass Valorisation and Protein Production in Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, TN-3038 Sfax, TunisiaWe report here the enhanced hemicellulase production by a Talaromyces thermophilus strain in a fed-batch fermentation using 3.6-litre laboratory-controlled bioreactor. When grown on wheat bran, this fungus produces a wide spectrum of polysaccharide-hydrolysing enzymes, mainly endo-β-1,4-xylanase (27 U/mL), β-xylosidase (1.4 U/mL), α-L-arabinofuranosidase (1.05 U/mL) and β-D-mannosidase (0.78 U/mL). The β-xylosidase was purified and shown to hydrolyse xylobiose and short xylooligosaccharides, but it was inactive on xylan. It released xylose from xylooligosaccharides with a degree of polymerisation ranging from 2 to 5. Talaromyces thermophilus β-xylosidase activity was unaffected by high glucose or arabinose concentration (0.5 M) and retained 75 % of its original activity in the presence of 133 mM xylose. Chitosan-immobilised β-xylosidase was used in a continuous process of conversion of wheat bran hydrolysate to xylose in a packed bed reactor. Xylose production of 18.6 mg/g was reached after six hours in the bioreactor and was twofold higher than that produced by the free enzyme. The produced xylose was further converted into xylitol using the crude intracellular enzyme of Talaromyces thermophilus.http://hrcak.srce.hr/file/169426hemicellulasesβ-xylosidasexylosefed-batch fermentationxylitol |
| spellingShingle | Ines Maalej-Achouri Hafedh Belghith Mohamed Guerfali Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production Food Technology and Biotechnology hemicellulases β-xylosidase xylose fed-batch fermentation xylitol |
| title | Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production |
| title_full | Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production |
| title_fullStr | Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production |
| title_full_unstemmed | Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production |
| title_short | Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production |
| title_sort | hydrolytic potential of talaromyces thermophilus β xylosidase and its use for continuous xylose production |
| topic | hemicellulases β-xylosidase xylose fed-batch fermentation xylitol |
| url | http://hrcak.srce.hr/file/169426 |
| work_keys_str_mv | AT inesmaalejachouri hydrolyticpotentialoftalaromycesthermophilusbxylosidaseanditsuseforcontinuousxyloseproduction AT hafedhbelghith hydrolyticpotentialoftalaromycesthermophilusbxylosidaseanditsuseforcontinuousxyloseproduction AT mohamedguerfali hydrolyticpotentialoftalaromycesthermophilusbxylosidaseanditsuseforcontinuousxyloseproduction |