Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs
Abstract Single-cell RNA sequencing has recently led to the identification of a flurry of rare, new cell types, such as the CFTR-high ionocytes in the airway epithelium. Ionocytes appear to be specifically responsible for fluid osmolarity and pH regulation. Similar cells exist in multiple other orga...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30603-1 |
| _version_ | 1827916986609303552 |
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| author | Carla Pou Casellas Cayetano Pleguezuelos-Manzano Maarten B. Rookmaaker Marianne C. Verhaar Hans Clevers |
| author_facet | Carla Pou Casellas Cayetano Pleguezuelos-Manzano Maarten B. Rookmaaker Marianne C. Verhaar Hans Clevers |
| author_sort | Carla Pou Casellas |
| collection | DOAJ |
| description | Abstract Single-cell RNA sequencing has recently led to the identification of a flurry of rare, new cell types, such as the CFTR-high ionocytes in the airway epithelium. Ionocytes appear to be specifically responsible for fluid osmolarity and pH regulation. Similar cells exist in multiple other organs and have received various names, including intercalated cell in the kidney, mitochondria-rich cell in the inner ear, clear cell in the epididymis, and ionocyte in the salivary gland. Here, we compare the previously published transcriptomic profile of cells expressing FOXI1, the signature transcription factor expressed in airway ionocytes. Such FOXI1+ cells were found in datasets representing human and/or murine kidney, airway, epididymis, thymus, skin, inner ear, salivary gland, and prostate. This allowed us to assess the similarities between these cells and identify the core transcriptomic signature of this ionocyte ‘family’. Our results demonstrate that, across all these organs, ionocytes maintain the expression of a characteristic set of genes, including FOXI1, KRT7, and ATP6V1B1. We conclude that the ionocyte signature defines a class of closely related cell types across multiple mammalian organs. |
| first_indexed | 2024-03-13T03:22:33Z |
| format | Article |
| id | doaj.art-c87fad1e558047af81e4a39f6de2362b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:22:33Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c87fad1e558047af81e4a39f6de2362b2023-06-25T11:16:03ZengNature PortfolioScientific Reports2045-23222023-03-0113111110.1038/s41598-023-30603-1Transcriptomic profile comparison reveals conservation of ionocytes across multiple organsCarla Pou Casellas0Cayetano Pleguezuelos-Manzano1Maarten B. Rookmaaker2Marianne C. Verhaar3Hans Clevers4Oncode Institute, Hubrecht Institute, Royal Dutch Academy of Science (KNAW) and University Medical Center Utrecht (UMCU)Oncode Institute, Hubrecht Institute, Royal Dutch Academy of Science (KNAW) and University Medical Center Utrecht (UMCU)Department of Nephrology and Hypertension, University Medical Center Utrecht (UMCU)Department of Nephrology and Hypertension, University Medical Center Utrecht (UMCU)Oncode Institute, Hubrecht Institute, Royal Dutch Academy of Science (KNAW) and University Medical Center Utrecht (UMCU)Abstract Single-cell RNA sequencing has recently led to the identification of a flurry of rare, new cell types, such as the CFTR-high ionocytes in the airway epithelium. Ionocytes appear to be specifically responsible for fluid osmolarity and pH regulation. Similar cells exist in multiple other organs and have received various names, including intercalated cell in the kidney, mitochondria-rich cell in the inner ear, clear cell in the epididymis, and ionocyte in the salivary gland. Here, we compare the previously published transcriptomic profile of cells expressing FOXI1, the signature transcription factor expressed in airway ionocytes. Such FOXI1+ cells were found in datasets representing human and/or murine kidney, airway, epididymis, thymus, skin, inner ear, salivary gland, and prostate. This allowed us to assess the similarities between these cells and identify the core transcriptomic signature of this ionocyte ‘family’. Our results demonstrate that, across all these organs, ionocytes maintain the expression of a characteristic set of genes, including FOXI1, KRT7, and ATP6V1B1. We conclude that the ionocyte signature defines a class of closely related cell types across multiple mammalian organs.https://doi.org/10.1038/s41598-023-30603-1 |
| spellingShingle | Carla Pou Casellas Cayetano Pleguezuelos-Manzano Maarten B. Rookmaaker Marianne C. Verhaar Hans Clevers Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs Scientific Reports |
| title | Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| title_full | Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| title_fullStr | Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| title_full_unstemmed | Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| title_short | Transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| title_sort | transcriptomic profile comparison reveals conservation of ionocytes across multiple organs |
| url | https://doi.org/10.1038/s41598-023-30603-1 |
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