DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis
Chuanyu Ma,1 Ning Ma,1 Lili Qin,1 Chuanna Miao,1 Minglei Luo,1 Shuhong Liu2 1Department of Proctology, Linyi Central Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Radiotherapy, Linyi Cancer Hospital, Linyi, Shandong Province, People’s Republic of...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2020-10-01
|
| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/dicer1-as1-promotes-the-malignant-behaviors-of-colorectal-cancer-cells-peer-reviewed-article-CMAR |
| _version_ | 1811193062262571008 |
|---|---|
| author | Ma C Ma N Qin L Miao C Luo M Liu S |
| author_facet | Ma C Ma N Qin L Miao C Luo M Liu S |
| author_sort | Ma C |
| collection | DOAJ |
| description | Chuanyu Ma,1 Ning Ma,1 Lili Qin,1 Chuanna Miao,1 Minglei Luo,1 Shuhong Liu2 1Department of Proctology, Linyi Central Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Radiotherapy, Linyi Cancer Hospital, Linyi, Shandong Province, People’s Republic of ChinaCorrespondence: Shuhong LiuDepartment of Radiotherapy, Linyi Cancer Hospital, Lingyuan East Street No. 6, Lanshan District, Linyi 276400, Shandong Province, People’s Republic of ChinaEmail yiyan0663363@163.comBackground: Long non-coding RNA (lncRNA) exerts a regulatory role in the occurrence and progression of tumors. This study aimed at probing into the function and mechanism of lncRNA DICER1 antisense RNA 1 (DICER1-AS1) in colorectal cancer (CRC).Methods: The expressions of DICER1-AS1, miR-296-5p and STAT3 mRNA were tested by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation, and Transwell was used to detect cell migration and invasion. In addition, the expressions of apoptosis-related proteins Bax and Bcl2 were detected by Western blot. Interactions between DICER1-AS1 and miR-296-5p, and miR-296-5p and STAT3 were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA binding protein immunoprecipitation (RIP) assay.Results: The expressions of DICER1-AS1 and STAT3 mRNA were significantly up-regulated while miR-296-5p expression was remarkably down-regulated in CRC tissues and cell lines. Over-expression of DICER1-AS1 or transfection of miR-296-5p inhibitors could promote the proliferation, migration and invasion and inhibit apoptosis of CRC cells, whereas knockdown of DICER1-AS1 or transfection of miR-296-5p mimics had the opposite effects. Additionally, DICER1-AS1 could down-regulate miR-296-5p expression via sponging it. DICER1-AS1 also enhanced the expression of STAT3, which was identified as a target gene of miR-296-5p.Conclusion: DICER1-AS1 acts as an oncogenic lncRNA in CRC via modulating miR-296-5p/STAT3 axis. Our results provide a new direction for the diagnosis and treatment of CRC.Keywords: DICER1-AS1, miR-296-5p, CRC, proliferation, metastasis |
| first_indexed | 2024-04-12T00:02:13Z |
| format | Article |
| id | doaj.art-c8819dcb0c00459a960c18a4cab0837f |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-04-12T00:02:13Z |
| publishDate | 2020-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-c8819dcb0c00459a960c18a4cab0837f2022-12-22T03:56:12ZengDove Medical PressCancer Management and Research1179-13222020-10-01Volume 12100351004658042DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 AxisMa CMa NQin LMiao CLuo MLiu SChuanyu Ma,1 Ning Ma,1 Lili Qin,1 Chuanna Miao,1 Minglei Luo,1 Shuhong Liu2 1Department of Proctology, Linyi Central Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Radiotherapy, Linyi Cancer Hospital, Linyi, Shandong Province, People’s Republic of ChinaCorrespondence: Shuhong LiuDepartment of Radiotherapy, Linyi Cancer Hospital, Lingyuan East Street No. 6, Lanshan District, Linyi 276400, Shandong Province, People’s Republic of ChinaEmail yiyan0663363@163.comBackground: Long non-coding RNA (lncRNA) exerts a regulatory role in the occurrence and progression of tumors. This study aimed at probing into the function and mechanism of lncRNA DICER1 antisense RNA 1 (DICER1-AS1) in colorectal cancer (CRC).Methods: The expressions of DICER1-AS1, miR-296-5p and STAT3 mRNA were tested by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation, and Transwell was used to detect cell migration and invasion. In addition, the expressions of apoptosis-related proteins Bax and Bcl2 were detected by Western blot. Interactions between DICER1-AS1 and miR-296-5p, and miR-296-5p and STAT3 were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA binding protein immunoprecipitation (RIP) assay.Results: The expressions of DICER1-AS1 and STAT3 mRNA were significantly up-regulated while miR-296-5p expression was remarkably down-regulated in CRC tissues and cell lines. Over-expression of DICER1-AS1 or transfection of miR-296-5p inhibitors could promote the proliferation, migration and invasion and inhibit apoptosis of CRC cells, whereas knockdown of DICER1-AS1 or transfection of miR-296-5p mimics had the opposite effects. Additionally, DICER1-AS1 could down-regulate miR-296-5p expression via sponging it. DICER1-AS1 also enhanced the expression of STAT3, which was identified as a target gene of miR-296-5p.Conclusion: DICER1-AS1 acts as an oncogenic lncRNA in CRC via modulating miR-296-5p/STAT3 axis. Our results provide a new direction for the diagnosis and treatment of CRC.Keywords: DICER1-AS1, miR-296-5p, CRC, proliferation, metastasishttps://www.dovepress.com/dicer1-as1-promotes-the-malignant-behaviors-of-colorectal-cancer-cells-peer-reviewed-article-CMARdicer1-as1mir-296-5pcrcproliferationmetastasis |
| spellingShingle | Ma C Ma N Qin L Miao C Luo M Liu S DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis Cancer Management and Research dicer1-as1 mir-296-5p crc proliferation metastasis |
| title | DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis |
| title_full | DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis |
| title_fullStr | DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis |
| title_full_unstemmed | DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis |
| title_short | DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis |
| title_sort | dicer1 as1 promotes the malignant behaviors of colorectal cancer cells by regulating mir 296 5p stat3 axis |
| topic | dicer1-as1 mir-296-5p crc proliferation metastasis |
| url | https://www.dovepress.com/dicer1-as1-promotes-the-malignant-behaviors-of-colorectal-cancer-cells-peer-reviewed-article-CMAR |
| work_keys_str_mv | AT mac dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis AT man dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis AT qinl dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis AT miaoc dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis AT luom dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis AT lius dicer1as1promotesthemalignantbehaviorsofcolorectalcancercellsbyregulatingmir2965pstat3axis |