Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis
Aim: Classical Hodgkin lymphoma is common, it is one of the lymphomas whose differential diagnosis can be difficult. It is thought that Hodgkin cell may originate from the germinal center. Our aim in this study was to determine the germinal center transformation markers OCT-2, BOB.1, BCL-6, PAX-5,...
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| Format: | Article |
| Language: | English |
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Rabia Yılmaz
2023-01-01
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| Series: | Journal of Contemporary Medicine |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/2851902 |
| _version_ | 1797860705368539136 |
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| author | Yiğit Şenol Filiz Yavaşoğlu Çiğdem Özdemir |
| author_facet | Yiğit Şenol Filiz Yavaşoğlu Çiğdem Özdemir |
| author_sort | Yiğit Şenol |
| collection | DOAJ |
| description | Aim: Classical Hodgkin lymphoma is common, it is one of the lymphomas whose differential diagnosis can be difficult. It is thought that Hodgkin cell may originate from the germinal center. Our aim in this study was to determine the germinal center transformation markers OCT-2, BOB.1, BCL-6, PAX-5, CD20 and MUM-1 in Classic Hodgkin Lymphoma (CHL), Nodular Lymphocyte Predominant Hodgkin Lymphoma (NLPHL) and Diffuse Large B-cell Lymphoma (DLBCL) to evaluate the expressions of by immunohistochemical method and chromogenic in-situ hybridization (ISH) of EBV early RNAs (EBER).
Material and methods: 49 biopsies diagnosed with Hodgkin lymphoma (HL), 5 with NLPHL and 19 with DLBCL were evaluated for CD30, PAX-5, OCT-2, BOB.1, MUM-1, BCL-6, and CD20, and EBER positivity. SPSS 18 was used for statistical analysis.
Results: 73 lymphoma cases were included in the study, 61.6% males and 38.4% females. The median age of patients was 50 years. CHL (67.1%) was the most common lymphoma type, and mixed cellular Hodgkin lymphoma (MSHL) was the most common subtype. There was a statistically significant difference in CD30, OCT-2, BOB.1, MUM-1, PAX-5, CD20, BCL-6, EBER expression between CHL and DLBCL cases (p |
| first_indexed | 2024-04-09T21:51:01Z |
| format | Article |
| id | doaj.art-c881dad0eade4862b616b3ffa0aa6a9e |
| institution | Directory Open Access Journal |
| issn | 2667-7180 |
| language | English |
| last_indexed | 2024-04-09T21:51:01Z |
| publishDate | 2023-01-01 |
| publisher | Rabia Yılmaz |
| record_format | Article |
| series | Journal of Contemporary Medicine |
| spelling | doaj.art-c881dad0eade4862b616b3ffa0aa6a9e2023-03-24T19:43:21ZengRabia YılmazJournal of Contemporary Medicine2667-71802023-01-01131929910.16899/jcm.12241161809Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential DiagnosisYiğit Şenol0Filiz Yavaşoğlu1Çiğdem Özdemir2AFYONKARAHISAR HEALTH SCIENCES UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF PUBLIC HEALTHESKISEHIR OSMANGAZI UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, HEMATOLOGYAFYONKARAHISAR HEALTH SCIENCES UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGICAL MEDICAL SCIENCES, DEPARTMENT OF PATHOLOGYAim: Classical Hodgkin lymphoma is common, it is one of the lymphomas whose differential diagnosis can be difficult. It is thought that Hodgkin cell may originate from the germinal center. Our aim in this study was to determine the germinal center transformation markers OCT-2, BOB.1, BCL-6, PAX-5, CD20 and MUM-1 in Classic Hodgkin Lymphoma (CHL), Nodular Lymphocyte Predominant Hodgkin Lymphoma (NLPHL) and Diffuse Large B-cell Lymphoma (DLBCL) to evaluate the expressions of by immunohistochemical method and chromogenic in-situ hybridization (ISH) of EBV early RNAs (EBER). Material and methods: 49 biopsies diagnosed with Hodgkin lymphoma (HL), 5 with NLPHL and 19 with DLBCL were evaluated for CD30, PAX-5, OCT-2, BOB.1, MUM-1, BCL-6, and CD20, and EBER positivity. SPSS 18 was used for statistical analysis. Results: 73 lymphoma cases were included in the study, 61.6% males and 38.4% females. The median age of patients was 50 years. CHL (67.1%) was the most common lymphoma type, and mixed cellular Hodgkin lymphoma (MSHL) was the most common subtype. There was a statistically significant difference in CD30, OCT-2, BOB.1, MUM-1, PAX-5, CD20, BCL-6, EBER expression between CHL and DLBCL cases (phttps://dergipark.org.tr/tr/download/article-file/2851902hodgkin’s lymphomaoct-2bob.1pax-5eberhodgkin’s lymphomabob.1oct-2eberpax-5 |
| spellingShingle | Yiğit Şenol Filiz Yavaşoğlu Çiğdem Özdemir Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis Journal of Contemporary Medicine hodgkin’s lymphoma oct-2 bob.1 pax-5 eber hodgkin’s lymphoma bob.1 oct-2 eber pax-5 |
| title | Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis |
| title_full | Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis |
| title_fullStr | Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis |
| title_full_unstemmed | Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis |
| title_short | Immunohistochemical Expression of B Cell Transcription Factors in Hodgkin’s Lymphoma and Their Use in Differential Diagnosis |
| title_sort | immunohistochemical expression of b cell transcription factors in hodgkin s lymphoma and their use in differential diagnosis |
| topic | hodgkin’s lymphoma oct-2 bob.1 pax-5 eber hodgkin’s lymphoma bob.1 oct-2 eber pax-5 |
| url | https://dergipark.org.tr/tr/download/article-file/2851902 |
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