A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein
Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Developmen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/5/157 |
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| author | Bárbara V. M. Silva Marli T. Cordeiro Marco A. B. Rodrigues Ernesto T. A. Marques Rosa F. Dutra |
| author_facet | Bárbara V. M. Silva Marli T. Cordeiro Marco A. B. Rodrigues Ernesto T. A. Marques Rosa F. Dutra |
| author_sort | Bárbara V. M. Silva |
| collection | DOAJ |
| description | Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube–polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6/36 and in Vero cell cultures infected with ZIKV. |
| first_indexed | 2024-03-10T11:22:21Z |
| format | Article |
| id | doaj.art-c881f4ed508f4a3cb80c5f66b58a5628 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T11:22:21Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-c881f4ed508f4a3cb80c5f66b58a56282023-11-21T19:57:48ZengMDPI AGBiosensors2079-63742021-05-0111515710.3390/bios11050157A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B ProteinBárbara V. M. Silva0Marli T. Cordeiro1Marco A. B. Rodrigues2Ernesto T. A. Marques3Rosa F. Dutra4Biomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Recife 50670-901, BrazilDepartment of Virology and Experimental Therapy, Oswaldo Cruz Foundation—FIOCRUZ, Aggeu Magalhães Institute, Recife 50670-420, BrazilDepartment of Electronics and Systems, Federal University of Pernambuco, Recife 50670-901, BrazilCenter for Vaccine Research, Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USABiomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Recife 50670-901, BrazilZika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube–polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6/36 and in Vero cell cultures infected with ZIKV.https://www.mdpi.com/2079-6374/11/5/157Zika virusNS2BPrussian bluepoint-of-care testingprobelessreagentless |
| spellingShingle | Bárbara V. M. Silva Marli T. Cordeiro Marco A. B. Rodrigues Ernesto T. A. Marques Rosa F. Dutra A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein Biosensors Zika virus NS2B Prussian blue point-of-care testing probeless reagentless |
| title | A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein |
| title_full | A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein |
| title_fullStr | A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein |
| title_full_unstemmed | A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein |
| title_short | A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein |
| title_sort | label and probe free zika virus immunosensor prussian blue carbon nanotube based for amperometric detection of the ns2b protein |
| topic | Zika virus NS2B Prussian blue point-of-care testing probeless reagentless |
| url | https://www.mdpi.com/2079-6374/11/5/157 |
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