Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Abstract Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purifi...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-10-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-022-00594-4 |
| _version_ | 1797996104497758208 |
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| author | Lilia C. Rojas-Pérez Paulo C. Narváez-Rincón M. Angélica M. Rocha Elisabete Coelho Manuel A. Coimbra |
| author_facet | Lilia C. Rojas-Pérez Paulo C. Narváez-Rincón M. Angélica M. Rocha Elisabete Coelho Manuel A. Coimbra |
| author_sort | Lilia C. Rojas-Pérez |
| collection | DOAJ |
| description | Abstract Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purified enzymes—endo-1,4-β-xylanase, α-l-arabinofuranosidase, β-xylosidase, and α-d-glucuronidase—over hydrolysis of glucuronoarabinoxylan (GAX) obtained from brewers’ spent grain (BSG) after alkaline extraction and ethanol precipitation. First, monosaccharides, uronic acids and glycosidic-linkages of alkaline extracted GAX fraction from BSG were characterized, after that different strategies based on the addition of one or two families of enzymes—endo-1,4-β-xylanase (GH10 and GH11) and α-l-arabinofuranosidase (GH43 and GH51)—cooperating with one β-xylosidase (GH43) and one α-d-glucuronidase (GH67) into enzymatic hydrolysis were assessed to obtain the best yield of xylose. The xylose release was monitored over time in the first 90 min and after a prolonged reaction up to 48 h of reaction. The highest yield of xylose was 63.6% (48 h, 40 ℃, pH 5.5), using a mixture of all enzymes devoid of α-l-arabinofuranosidase (GH43) family. These results highlight the importance of GH51 arabinofuranosidase debranching enzyme to allow a higher cleavage of the xylan backbone of GAX from BSG and their synergy with 2 endo-1,4-β-xylanase (GH10 and GH11), one β-xylosidase (GH43) and the inclusion of one α-d-glucuronidase (GH67) in the reaction system. Therefore, this study provides an environmentally friendly process to produce xylose from BSG through utilization of enzymes as catalysts. Graphical Abstract |
| first_indexed | 2024-04-11T10:12:12Z |
| format | Article |
| id | doaj.art-c8825b5ff10f46e1a0c7d06e389570db |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-11T10:12:12Z |
| publishDate | 2022-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-c8825b5ff10f46e1a0c7d06e389570db2022-12-22T04:30:05ZengSpringerOpenBioresources and Bioprocessing2197-43652022-10-01911910.1186/s40643-022-00594-4Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grainLilia C. Rojas-Pérez0Paulo C. Narváez-Rincón1M. Angélica M. Rocha2Elisabete Coelho3Manuel A. Coimbra4Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad EanDepartamento de Ingeniería Química y Ambiental, Facultad de Ingeniería, Universidad Nacional de ColombiaDepartamento de Química, Universidade de AveiroDepartamento de Química, Universidade de AveiroDepartamento de Química, Universidade de AveiroAbstract Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purified enzymes—endo-1,4-β-xylanase, α-l-arabinofuranosidase, β-xylosidase, and α-d-glucuronidase—over hydrolysis of glucuronoarabinoxylan (GAX) obtained from brewers’ spent grain (BSG) after alkaline extraction and ethanol precipitation. First, monosaccharides, uronic acids and glycosidic-linkages of alkaline extracted GAX fraction from BSG were characterized, after that different strategies based on the addition of one or two families of enzymes—endo-1,4-β-xylanase (GH10 and GH11) and α-l-arabinofuranosidase (GH43 and GH51)—cooperating with one β-xylosidase (GH43) and one α-d-glucuronidase (GH67) into enzymatic hydrolysis were assessed to obtain the best yield of xylose. The xylose release was monitored over time in the first 90 min and after a prolonged reaction up to 48 h of reaction. The highest yield of xylose was 63.6% (48 h, 40 ℃, pH 5.5), using a mixture of all enzymes devoid of α-l-arabinofuranosidase (GH43) family. These results highlight the importance of GH51 arabinofuranosidase debranching enzyme to allow a higher cleavage of the xylan backbone of GAX from BSG and their synergy with 2 endo-1,4-β-xylanase (GH10 and GH11), one β-xylosidase (GH43) and the inclusion of one α-d-glucuronidase (GH67) in the reaction system. Therefore, this study provides an environmentally friendly process to produce xylose from BSG through utilization of enzymes as catalysts. Graphical Abstracthttps://doi.org/10.1186/s40643-022-00594-4Brewers’ spent grainGlucuronoarabinoxylanArabinoxylanXylan saccharificationSynergismXylanolytic enzymes |
| spellingShingle | Lilia C. Rojas-Pérez Paulo C. Narváez-Rincón M. Angélica M. Rocha Elisabete Coelho Manuel A. Coimbra Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain Bioresources and Bioprocessing Brewers’ spent grain Glucuronoarabinoxylan Arabinoxylan Xylan saccharification Synergism Xylanolytic enzymes |
| title | Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain |
| title_full | Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain |
| title_fullStr | Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain |
| title_full_unstemmed | Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain |
| title_short | Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain |
| title_sort | production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers spent grain |
| topic | Brewers’ spent grain Glucuronoarabinoxylan Arabinoxylan Xylan saccharification Synergism Xylanolytic enzymes |
| url | https://doi.org/10.1186/s40643-022-00594-4 |
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