A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195
The altered expression of long noncoding RNAs (lncRNAs) is associated with human carcinogenesis. We performed a high-throughput analysis of lncRNA expression in strictly selected pairs of metastatic head and neck squamous cell carcinoma (HNSCC) and non-metastatic HNSCC samples. We identified a novel...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/24/5792 |
| _version_ | 1797381690339885056 |
|---|---|
| author | Yujia Wang Zhen Qin Yiwen Chen Yunfei Zheng Lingfei Jia |
| author_facet | Yujia Wang Zhen Qin Yiwen Chen Yunfei Zheng Lingfei Jia |
| author_sort | Yujia Wang |
| collection | DOAJ |
| description | The altered expression of long noncoding RNAs (lncRNAs) is associated with human carcinogenesis. We performed a high-throughput analysis of lncRNA expression in strictly selected pairs of metastatic head and neck squamous cell carcinoma (HNSCC) and non-metastatic HNSCC samples. We identified a novel lncRNA, which was highly expressed in metastatic HNSCC, named Metastasis Associated Squamous Cell Carcinoma 1 (MASCC1), for further study. Using qRT-PCR, we further compared MASCC1 expression in 60 HNSCC samples. The results show that high expression of MASCC1 in patients with HNSCC was related to poor prognosis. In vitro, MASCC1 knockdown (KD) inhibited HNSCC proliferation, migration, invasion, and tumor sphere formation, while promoting apoptosis. In vivo, MASCC1 KD inhibited HNSCC growth and lymph node metastasis. Mechanistically, MASCC1 acted as a competing endogenous RNA (ceRNA) by binding to miR-195, subsequently regulating the expression of Cyclin D1, BCL-2, and YAP1. Moreover, miR-195 overexpression rescued the effects of MASCC1 on the biological behaviors of HNSCC. Taken together, our results suggest that <i>MASCC1</i> is a novel oncogene that can predict the prognosis of patients with HNSCC and is a potential therapeutic target for HNSCC intervention. |
| first_indexed | 2024-03-08T20:56:05Z |
| format | Article |
| id | doaj.art-c8887f56a0684d61bde5d64936821a94 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T20:56:05Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c8887f56a0684d61bde5d64936821a942023-12-22T13:58:53ZengMDPI AGCancers2072-66942023-12-011524579210.3390/cancers15245792A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195Yujia Wang0Zhen Qin1Yiwen Chen2Yunfei Zheng3Lingfei Jia4Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaThe altered expression of long noncoding RNAs (lncRNAs) is associated with human carcinogenesis. We performed a high-throughput analysis of lncRNA expression in strictly selected pairs of metastatic head and neck squamous cell carcinoma (HNSCC) and non-metastatic HNSCC samples. We identified a novel lncRNA, which was highly expressed in metastatic HNSCC, named Metastasis Associated Squamous Cell Carcinoma 1 (MASCC1), for further study. Using qRT-PCR, we further compared MASCC1 expression in 60 HNSCC samples. The results show that high expression of MASCC1 in patients with HNSCC was related to poor prognosis. In vitro, MASCC1 knockdown (KD) inhibited HNSCC proliferation, migration, invasion, and tumor sphere formation, while promoting apoptosis. In vivo, MASCC1 KD inhibited HNSCC growth and lymph node metastasis. Mechanistically, MASCC1 acted as a competing endogenous RNA (ceRNA) by binding to miR-195, subsequently regulating the expression of Cyclin D1, BCL-2, and YAP1. Moreover, miR-195 overexpression rescued the effects of MASCC1 on the biological behaviors of HNSCC. Taken together, our results suggest that <i>MASCC1</i> is a novel oncogene that can predict the prognosis of patients with HNSCC and is a potential therapeutic target for HNSCC intervention.https://www.mdpi.com/2072-6694/15/24/5792head and neck squamous cell carcinoma (HNSCC)lncRNAmetastasis associated squamous cell carcinoma 1 (MASCC1)miRNAmiR-195progression |
| spellingShingle | Yujia Wang Zhen Qin Yiwen Chen Yunfei Zheng Lingfei Jia A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 Cancers head and neck squamous cell carcinoma (HNSCC) lncRNA metastasis associated squamous cell carcinoma 1 (MASCC1) miRNA miR-195 progression |
| title | A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 |
| title_full | A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 |
| title_fullStr | A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 |
| title_full_unstemmed | A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 |
| title_short | A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195 |
| title_sort | novel lncrna mascc1 regulates the progression and metastasis of head and neck squamous cell carcinoma by sponging mir 195 |
| topic | head and neck squamous cell carcinoma (HNSCC) lncRNA metastasis associated squamous cell carcinoma 1 (MASCC1) miRNA miR-195 progression |
| url | https://www.mdpi.com/2072-6694/15/24/5792 |
| work_keys_str_mv | AT yujiawang anovellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT zhenqin anovellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT yiwenchen anovellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT yunfeizheng anovellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT lingfeijia anovellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT yujiawang novellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT zhenqin novellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT yiwenchen novellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT yunfeizheng novellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 AT lingfeijia novellncrnamascc1regulatestheprogressionandmetastasisofheadandnecksquamouscellcarcinomabyspongingmir195 |