Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax
The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100016 |
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| author | Joana D'Arc Neves Costa Fernando Berton Zanchi Francisco Lurdevanhe da Silva Rodrigues Eduardo Rezende Honda Tony Hiroschi Katsuragawa Dhélio Batista Pereira Roger Lafontaine Mesquita Taborda Mauro Shugiro Tada Ricardo de Godoi Mattos Ferreira Luiz Hildebrando Pereira-da-Silva |
| author_facet | Joana D'Arc Neves Costa Fernando Berton Zanchi Francisco Lurdevanhe da Silva Rodrigues Eduardo Rezende Honda Tony Hiroschi Katsuragawa Dhélio Batista Pereira Roger Lafontaine Mesquita Taborda Mauro Shugiro Tada Ricardo de Godoi Mattos Ferreira Luiz Hildebrando Pereira-da-Silva |
| author_sort | Joana D'Arc Neves Costa |
| collection | DOAJ |
| description | The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfCLAG9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections. |
| first_indexed | 2024-03-12T18:30:18Z |
| format | Article |
| id | doaj.art-c888fb38d5a848b687e717e7aa783798 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:30:18Z |
| publishDate | 2013-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c888fb38d5a848b687e717e7aa7837982023-08-02T08:19:27ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602013-02-01108198105Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivaxJoana D'Arc Neves CostaFernando Berton ZanchiFrancisco Lurdevanhe da Silva RodriguesEduardo Rezende HondaTony Hiroschi KatsuragawaDhélio Batista PereiraRoger Lafontaine Mesquita TabordaMauro Shugiro TadaRicardo de Godoi Mattos FerreiraLuiz Hildebrando Pereira-da-SilvaThe PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfCLAG9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100016malariaimmunityAmazoncross-reaction |
| spellingShingle | Joana D'Arc Neves Costa Fernando Berton Zanchi Francisco Lurdevanhe da Silva Rodrigues Eduardo Rezende Honda Tony Hiroschi Katsuragawa Dhélio Batista Pereira Roger Lafontaine Mesquita Taborda Mauro Shugiro Tada Ricardo de Godoi Mattos Ferreira Luiz Hildebrando Pereira-da-Silva Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax Memorias do Instituto Oswaldo Cruz malaria immunity Amazon cross-reaction |
| title | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax |
| title_full | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax |
| title_fullStr | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax |
| title_full_unstemmed | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax |
| title_short | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax |
| title_sort | cross reactive anti pfclag9 antibodies in the sera of asymptomatic parasite carriers of plasmodium vivax |
| topic | malaria immunity Amazon cross-reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100016 |
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