Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis.
Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-deriv...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-10-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010814 |
| _version_ | 1798029265917181952 |
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| author | Xiaola Guo Shuai Wang Junmei Zhang Rui Li Yong'e Zhang Zhengrong Wang Qingming Kong William C Cho Xianghong Ju Yujuan Shen Lingqiang Zhang Haining Fan Jianping Cao Yadong Zheng |
| author_facet | Xiaola Guo Shuai Wang Junmei Zhang Rui Li Yong'e Zhang Zhengrong Wang Qingming Kong William C Cho Xianghong Ju Yujuan Shen Lingqiang Zhang Haining Fan Jianping Cao Yadong Zheng |
| author_sort | Xiaola Guo |
| collection | DOAJ |
| description | Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis. |
| first_indexed | 2024-04-11T19:21:22Z |
| format | Article |
| id | doaj.art-c88e232a4a724acb9d7eab4715636839 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-11T19:21:22Z |
| publishDate | 2022-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-c88e232a4a724acb9d7eab47156368392022-12-22T04:07:17ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-10-011610e001081410.1371/journal.pntd.0010814Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis.Xiaola GuoShuai WangJunmei ZhangRui LiYong'e ZhangZhengrong WangQingming KongWilliam C ChoXianghong JuYujuan ShenLingqiang ZhangHaining FanJianping CaoYadong ZhengEchinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis.https://doi.org/10.1371/journal.pntd.0010814 |
| spellingShingle | Xiaola Guo Shuai Wang Junmei Zhang Rui Li Yong'e Zhang Zhengrong Wang Qingming Kong William C Cho Xianghong Ju Yujuan Shen Lingqiang Zhang Haining Fan Jianping Cao Yadong Zheng Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. PLoS Neglected Tropical Diseases |
| title | Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. |
| title_full | Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. |
| title_fullStr | Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. |
| title_full_unstemmed | Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. |
| title_short | Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. |
| title_sort | proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis |
| url | https://doi.org/10.1371/journal.pntd.0010814 |
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