Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization
One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, syn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2017-02-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291401/ |
| _version_ | 1828312256934313984 |
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| author | Kazuyoshi Furuta Saki Nagashima Tsuyoshi Inukai Chikara Masuta |
| author_facet | Kazuyoshi Furuta Saki Nagashima Tsuyoshi Inukai Chikara Masuta |
| author_sort | Kazuyoshi Furuta |
| collection | DOAJ |
| description | One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge. |
| first_indexed | 2024-04-13T16:12:12Z |
| format | Article |
| id | doaj.art-c890b98ce5dc43e3ad4d5789ebfcc0fe |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-04-13T16:12:12Z |
| publishDate | 2017-02-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-c890b98ce5dc43e3ad4d5789ebfcc0fe2022-12-22T02:40:11ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-02-01331808610.5423/PPJ.NT.05.2016.0132PPJ.NT.05.2016.0132Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube HybridizationKazuyoshi Furuta0Saki Nagashima1Tsuyoshi Inukai2Chikara Masuta3Agroscience Research Laboratories, Plant Biotechnology Center, Hokusan Co., Ltd., Kitahiroshima 061-1111, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanOne of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291401/Colletotrichummicrotube-hybridizationstrawberry |
| spellingShingle | Kazuyoshi Furuta Saki Nagashima Tsuyoshi Inukai Chikara Masuta Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization The Plant Pathology Journal Colletotrichum microtube-hybridization strawberry |
| title | Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization |
| title_full | Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization |
| title_fullStr | Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization |
| title_full_unstemmed | Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization |
| title_short | Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization |
| title_sort | construction of a system for the strawberry nursery production towards elimination of latent infection of anthracnose fungi by a combination of pcr and microtube hybridization |
| topic | Colletotrichum microtube-hybridization strawberry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291401/ |
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