Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease
Acute graft-versus-host disease (aGVHD) represents a challenging complication after allogeneic hematopoietic stem cell transplantation. Despite the intensive preclinical research in the field of prevention and treatment of aGVHD, and the presence of a well-established clinical grading system to eval...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2018.00010/full |
| _version_ | 1818538017255587840 |
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| author | Sina Naserian Sina Naserian Mathieu Leclerc Mathieu Leclerc Mathieu Leclerc Allan Thiolat Allan Thiolat Caroline Pilon Caroline Pilon Caroline Pilon Cindy Le Bret Yazid Belkacemi Sébastien Maury Sébastien Maury Sébastien Maury Frédéric Charlotte José L. Cohen José L. Cohen José L. Cohen |
| author_facet | Sina Naserian Sina Naserian Mathieu Leclerc Mathieu Leclerc Mathieu Leclerc Allan Thiolat Allan Thiolat Caroline Pilon Caroline Pilon Caroline Pilon Cindy Le Bret Yazid Belkacemi Sébastien Maury Sébastien Maury Sébastien Maury Frédéric Charlotte José L. Cohen José L. Cohen José L. Cohen |
| author_sort | Sina Naserian |
| collection | DOAJ |
| description | Acute graft-versus-host disease (aGVHD) represents a challenging complication after allogeneic hematopoietic stem cell transplantation. Despite the intensive preclinical research in the field of prevention and treatment of aGVHD, and the presence of a well-established clinical grading system to evaluate human aGVHD, such a valid tool is still lacking for the evaluation of murine aGVHD. Indeed, several scoring systems have been reported, but none of them has been properly evaluated and they all share some limitations: they incompletely reflect the disease, rely on severity stages that are distinguished by subjective assessment of clinical criteria and are not easy to discriminate, which could render evaluation more time consuming, and their reproducibility among different experimenters is uncertain. Consequently, clinical murine aGVHD description is often based merely on animal weight loss and mortality. Here, we propose a simple scoring system of aGVHD relying on the binary (yes or no) evaluation of five important visual parameters that reflect the complexity of the disease without the need to sacrifice the mice. We show that this scoring system is consistent with the gold standard histological staging of aGVHD across several donor/recipient mice combinations. This system is also a strong predictor of survival of recipient mice when used early after transplant and is highly reproducible between experimenters. |
| first_indexed | 2024-12-11T18:58:11Z |
| format | Article |
| id | doaj.art-c893ecf540a14f3bae451c33320c1b69 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T18:58:11Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c893ecf540a14f3bae451c33320c1b692022-12-22T00:54:05ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-01-01910.3389/fimmu.2018.00010294150Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host DiseaseSina Naserian0Sina Naserian1Mathieu Leclerc2Mathieu Leclerc3Mathieu Leclerc4Allan Thiolat5Allan Thiolat6Caroline Pilon7Caroline Pilon8Caroline Pilon9Cindy Le Bret10Yazid Belkacemi11Sébastien Maury12Sébastien Maury13Sébastien Maury14Frédéric Charlotte15José L. Cohen16José L. Cohen17José L. Cohen18Université Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceUniversité Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceAPHP, Service d’hématologie Clinique, Hôpital Henri Mondor, Créteil, FranceUniversité Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceUniversité Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceUPEC, APHP, INSERM, CIC Biothérapie, Hôpital Henri Mondor, Créteil, FranceUniversité Paris-Est Créteil Val de Marne, APHP, Service d’Oncologie-Radiothérapie, Hôpital Henri Mondor, Créteil, FranceUniversité Paris-Est Créteil Val de Marne, APHP, Service d’Oncologie-Radiothérapie, Hôpital Henri Mondor, Créteil, FranceUniversité Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceAPHP, Service d’hématologie Clinique, Hôpital Henri Mondor, Créteil, FranceAPHP, Hôpital Pitié Salpêtrière, Service d’Anatomopathologie, Paris, FranceUniversité Paris-Est, UMR_S955, Université Paris-Est Créteil Val de Marne, Créteil, FranceINSERM, U955, Equipe 21, Créteil, FranceUPEC, APHP, INSERM, CIC Biothérapie, Hôpital Henri Mondor, Créteil, FranceAcute graft-versus-host disease (aGVHD) represents a challenging complication after allogeneic hematopoietic stem cell transplantation. Despite the intensive preclinical research in the field of prevention and treatment of aGVHD, and the presence of a well-established clinical grading system to evaluate human aGVHD, such a valid tool is still lacking for the evaluation of murine aGVHD. Indeed, several scoring systems have been reported, but none of them has been properly evaluated and they all share some limitations: they incompletely reflect the disease, rely on severity stages that are distinguished by subjective assessment of clinical criteria and are not easy to discriminate, which could render evaluation more time consuming, and their reproducibility among different experimenters is uncertain. Consequently, clinical murine aGVHD description is often based merely on animal weight loss and mortality. Here, we propose a simple scoring system of aGVHD relying on the binary (yes or no) evaluation of five important visual parameters that reflect the complexity of the disease without the need to sacrifice the mice. We show that this scoring system is consistent with the gold standard histological staging of aGVHD across several donor/recipient mice combinations. This system is also a strong predictor of survival of recipient mice when used early after transplant and is highly reproducible between experimenters.http://journal.frontiersin.org/article/10.3389/fimmu.2018.00010/fullacute graft-versus-host diseasemiceclinical gradingclassificationmurine modelsprognostic factor |
| spellingShingle | Sina Naserian Sina Naserian Mathieu Leclerc Mathieu Leclerc Mathieu Leclerc Allan Thiolat Allan Thiolat Caroline Pilon Caroline Pilon Caroline Pilon Cindy Le Bret Yazid Belkacemi Sébastien Maury Sébastien Maury Sébastien Maury Frédéric Charlotte José L. Cohen José L. Cohen José L. Cohen Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease Frontiers in Immunology acute graft-versus-host disease mice clinical grading classification murine models prognostic factor |
| title | Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease |
| title_full | Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease |
| title_fullStr | Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease |
| title_full_unstemmed | Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease |
| title_short | Simple, Reproducible, and Efficient Clinical Grading System for Murine Models of Acute Graft-versus-Host Disease |
| title_sort | simple reproducible and efficient clinical grading system for murine models of acute graft versus host disease |
| topic | acute graft-versus-host disease mice clinical grading classification murine models prognostic factor |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2018.00010/full |
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