Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed
This study was carried out to identify the behaviour of <i>Escherichia coli</i> O157:H7 and of <i>Listeria monocytogenes</i> inoculated in Maronesa breed beef with different ultimate pH (pHu) (Normal and DFD), and stored at two different temperatures (4 and 9 °C), during 28 d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1420 |
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| author | Cristina Saraiva Sónia Saraiva Luis Patarata Maria da Conceição Fontes Conceição Martins |
| author_facet | Cristina Saraiva Sónia Saraiva Luis Patarata Maria da Conceição Fontes Conceição Martins |
| author_sort | Cristina Saraiva |
| collection | DOAJ |
| description | This study was carried out to identify the behaviour of <i>Escherichia coli</i> O157:H7 and of <i>Listeria monocytogenes</i> inoculated in Maronesa breed beef with different ultimate pH (pHu) (Normal and DFD), and stored at two different temperatures (4 and 9 °C), during 28 days <i>post mortem</i> (pm). The main objective was to illustrate the problematic feature of dealing with beef showing high pHu and stored at mild abusive temperatures (9 °C). Beef steaks (ms. <i>longissimus dorsi</i>) were inoculated with low levels (2–3 log CFU/g) of those both pathogens and packed in air, vacuum and three gaseous mixtures with decreasing O<sub>2</sub> and increasing CO<sub>2</sub> concentrations (MAP70/20, MAP50/40 and MAP30/60). At 4 °C, the growth of <i>E. coli</i> O157:H7 presented the same pattern on Normal and DFD meat. On the contrary, the growth of <i>L. monocytogenes</i> was higher in DFD meat, revealing the effect of the pHu and its psychotropic character. At abusive temperatures, both pathogens grew, achieving high levels in DFD meat. In these cases, the MAP with the highest CO<sub>2</sub> concentration (60%) was revealed to be more effective against the development of <i>E. coli</i> O157:H7, therefore, not exceeding levels of 5 log CFU/g at the end of storage, while in <i>L. monocytogenes,</i> it reaches 8 log CFU/g under the same conditions. |
| first_indexed | 2024-03-11T05:38:08Z |
| format | Article |
| id | doaj.art-c89547265539405a8fbb02756d696940 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:38:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-c89547265539405a8fbb02756d6969402023-11-17T16:41:02ZengMDPI AGFoods2304-81582023-03-01127142010.3390/foods12071420Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese BreedCristina Saraiva0Sónia Saraiva1Luis Patarata2Maria da Conceição Fontes3Conceição Martins4Animal and Veterinary Science Center (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalAnimal and Veterinary Science Center (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalAnimal and Veterinary Science Center (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalAnimal and Veterinary Science Center (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalAnimal and Veterinary Science Center (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, PortugalThis study was carried out to identify the behaviour of <i>Escherichia coli</i> O157:H7 and of <i>Listeria monocytogenes</i> inoculated in Maronesa breed beef with different ultimate pH (pHu) (Normal and DFD), and stored at two different temperatures (4 and 9 °C), during 28 days <i>post mortem</i> (pm). The main objective was to illustrate the problematic feature of dealing with beef showing high pHu and stored at mild abusive temperatures (9 °C). Beef steaks (ms. <i>longissimus dorsi</i>) were inoculated with low levels (2–3 log CFU/g) of those both pathogens and packed in air, vacuum and three gaseous mixtures with decreasing O<sub>2</sub> and increasing CO<sub>2</sub> concentrations (MAP70/20, MAP50/40 and MAP30/60). At 4 °C, the growth of <i>E. coli</i> O157:H7 presented the same pattern on Normal and DFD meat. On the contrary, the growth of <i>L. monocytogenes</i> was higher in DFD meat, revealing the effect of the pHu and its psychotropic character. At abusive temperatures, both pathogens grew, achieving high levels in DFD meat. In these cases, the MAP with the highest CO<sub>2</sub> concentration (60%) was revealed to be more effective against the development of <i>E. coli</i> O157:H7, therefore, not exceeding levels of 5 log CFU/g at the end of storage, while in <i>L. monocytogenes,</i> it reaches 8 log CFU/g under the same conditions.https://www.mdpi.com/2304-8158/12/7/1420autochthonous breedbeefDFD meat<i>Listeria monocytogenes</i><i>Escherichia coli</i> O157:H7ultimate pH |
| spellingShingle | Cristina Saraiva Sónia Saraiva Luis Patarata Maria da Conceição Fontes Conceição Martins Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed Foods autochthonous breed beef DFD meat <i>Listeria monocytogenes</i> <i>Escherichia coli</i> O157:H7 ultimate pH |
| title | Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed |
| title_full | Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed |
| title_fullStr | Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed |
| title_full_unstemmed | Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed |
| title_short | Behaviour of <i>Escherichia coli</i> O157:H7 and <i>Listeria monocytogenes</i> in Normal and DFD Beef of an Autochthonous Portuguese Breed |
| title_sort | behaviour of i escherichia coli i o157 h7 and i listeria monocytogenes i in normal and dfd beef of an autochthonous portuguese breed |
| topic | autochthonous breed beef DFD meat <i>Listeria monocytogenes</i> <i>Escherichia coli</i> O157:H7 ultimate pH |
| url | https://www.mdpi.com/2304-8158/12/7/1420 |
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