Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of <i>Staphyloc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/2/117 |
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| author | Fernanda Soares Pessanha Beatriz Guitton Renaud Baptista de Oliveira Bianca Campos Oliveira Gabriela Deutsch Felipe Lopes Teixeira Luciana Castilho Bokehi Mariana Alcântara Calomino Selma Rodrigues de Castilho Rossana Mara da Silva Moreira Thiré Lenise Arneiro Teixeira Geraldo Renato de Paula |
| author_facet | Fernanda Soares Pessanha Beatriz Guitton Renaud Baptista de Oliveira Bianca Campos Oliveira Gabriela Deutsch Felipe Lopes Teixeira Luciana Castilho Bokehi Mariana Alcântara Calomino Selma Rodrigues de Castilho Rossana Mara da Silva Moreira Thiré Lenise Arneiro Teixeira Geraldo Renato de Paula |
| author_sort | Fernanda Soares Pessanha |
| collection | DOAJ |
| description | Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 <i>S. aureus</i> strains and 15 <i>P. aeruginosa</i> strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds. |
| first_indexed | 2024-03-11T08:48:16Z |
| format | Article |
| id | doaj.art-c89641dcd6b946d5ae24c598854e9288 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-11T08:48:16Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-c89641dcd6b946d5ae24c598854e92882023-11-16T20:39:26ZengMDPI AGGels2310-28612023-02-019211710.3390/gels9020117Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical TrialFernanda Soares Pessanha0Beatriz Guitton Renaud Baptista de Oliveira1Bianca Campos Oliveira2Gabriela Deutsch3Felipe Lopes Teixeira4Luciana Castilho Bokehi5Mariana Alcântara Calomino6Selma Rodrigues de Castilho7Rossana Mara da Silva Moreira Thiré8Lenise Arneiro Teixeira9Geraldo Renato de Paula10Nursing School, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551-030, BrazilAurora de Afonso Costa College of Nursing, Universidade Federal Fluminense, Niterói 24020-091, BrazilAurora de Afonso Costa College of Nursing, Universidade Federal Fluminense, Niterói 24020-091, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCOPPE/Program of Metallurgical and Materials Engineering—PEMM, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-599, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilCollege of Pharmacy, Universidade Federal Fluminense, Niterói 24241-000, BrazilDiabetic patients frequently develop wounds, which can be colonized by bacteria, mainly <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 <i>S. aureus</i> strains and 15 <i>P. aeruginosa</i> strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds.https://www.mdpi.com/2310-2861/9/2/117epidermal growth factorbiofilm<i>Staphylococcus aureus</i><i>Pseudomonas aeruginosa</i>diabetic foot |
| spellingShingle | Fernanda Soares Pessanha Beatriz Guitton Renaud Baptista de Oliveira Bianca Campos Oliveira Gabriela Deutsch Felipe Lopes Teixeira Luciana Castilho Bokehi Mariana Alcântara Calomino Selma Rodrigues de Castilho Rossana Mara da Silva Moreira Thiré Lenise Arneiro Teixeira Geraldo Renato de Paula Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial Gels epidermal growth factor biofilm <i>Staphylococcus aureus</i> <i>Pseudomonas aeruginosa</i> diabetic foot |
| title | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
| title_full | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
| title_fullStr | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
| title_full_unstemmed | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
| title_short | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
| title_sort | effectiveness of epidermal growth factor loaded carboxymethylcellulose egf cmc hydrogel in biofilm formation in wounds of diabetic patients a randomized clinical trial |
| topic | epidermal growth factor biofilm <i>Staphylococcus aureus</i> <i>Pseudomonas aeruginosa</i> diabetic foot |
| url | https://www.mdpi.com/2310-2861/9/2/117 |
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