Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis
The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2005-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000200015 |
| _version_ | 1827880436038107136 |
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| author | Eliza Yoshie do Rosário Odair Genaro João C França-Silva Roberto T da Costa Wilson Mayrink Alexandre Barbosa Reis Mariângela Carneiro |
| author_facet | Eliza Yoshie do Rosário Odair Genaro João C França-Silva Roberto T da Costa Wilson Mayrink Alexandre Barbosa Reis Mariângela Carneiro |
| author_sort | Eliza Yoshie do Rosário |
| collection | DOAJ |
| description | The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 1:40 titre, 106 (92.2%) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 1:40 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100%). Specificity was higher than 96% for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88%). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97). Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however. |
| first_indexed | 2024-03-12T18:22:28Z |
| format | Article |
| id | doaj.art-c89798b751dc4a2197f8214f353bf92f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:22:28Z |
| publishDate | 2005-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c89798b751dc4a2197f8214f353bf92f2023-08-02T08:46:54ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602005-04-01100219720310.1590/S0074-02762005000200015Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasisEliza Yoshie do RosárioOdair GenaroJoão C França-SilvaRoberto T da CostaWilson MayrinkAlexandre Barbosa ReisMariângela CarneiroThe performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 1:40 titre, 106 (92.2%) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 1:40 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100%). Specificity was higher than 96% for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88%). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97). Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000200015canine visceral leishmaniasisLeishmania (Leishmania) chagasidiagnosisrecombinant antigens |
| spellingShingle | Eliza Yoshie do Rosário Odair Genaro João C França-Silva Roberto T da Costa Wilson Mayrink Alexandre Barbosa Reis Mariângela Carneiro Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis Memorias do Instituto Oswaldo Cruz canine visceral leishmaniasis Leishmania (Leishmania) chagasi diagnosis recombinant antigens |
| title | Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| title_full | Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| title_fullStr | Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| title_full_unstemmed | Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| title_short | Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| title_sort | evaluation of enzyme linked immunosorbent assay using crude leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis |
| topic | canine visceral leishmaniasis Leishmania (Leishmania) chagasi diagnosis recombinant antigens |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000200015 |
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