Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres
The purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the vestibular epithelia of young mice. We harvested utricles and sacculi from 7 days old mice. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM w...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/604 |
| _version_ | 1827288022929899520 |
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| author | Roxana Vintilă Oana Gavriliuc |
| author_facet | Roxana Vintilă Oana Gavriliuc |
| author_sort | Roxana Vintilă |
| collection | DOAJ |
| description | The purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the vestibular epithelia of young mice.
We harvested utricles and sacculi from 7 days old mice. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM with F12 Nutrient mixture, B27, N2 supplement, IGF-1 and EGF. Sphere pluripotency was established with Nanog and Oct-4 stem cell markers. We mechanically dissociated primary spheres and cultivated them. Cells were characterized by immunofluorescence and immunohistochemistry for myosinVIIA (hair cell marker), nestin (intermediate filament VI marker) and beta III tubulin.
We proved that vestibular epithelia contains pluripotent stem cells which formed spheres. Sphere-derived cells’ pluripotency was demonstrated by the expression of nanog, oct 4 and nestin markers. Also, after sphere dissociation we obtained a higher number of spheres beeing pluripotent and capable of self-renewal. We obtained through differentiation different cell types including neuron like-cells which were positive for myosin VIIA, nestin and beta III tubulin.
Utricular epithelia of seven days old mice contains sufficient pluripotent stem cells which generate spheres.
Cells obtained from utricular epithelia are pluripotent because they express nanog, oct 4, nesThe purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the vestibular epithelia of young mice.
We harvested utricles and sacculi from 7 days old mice. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM with F12 Nutrient mixture, B27, N2 supplement, IGF-1 and EGF. Sphere pluripotency was established with Nanog and Oct-4 stem cell markers. We mechanically dissociated primary spheres and cultivated them. Cells were characterized by immunofluorescence and immunohistochemistry for myosinVIIA (hair cell marker), nestin (intermediate filament VI marker) and beta III tubulin.
We proved that vestibular epithelia contains pluripotent stem cells which formed spheres. Sphere-derived cells’ pluripotency was demonstrated by the expression of nanog, oct 4 and nestin markers. Also, after sphere dissociation we obtained a higher number of spheres beeing pluripotent and capable of self-renewal. We obtained through differentiation different cell types including neuron like-cells which were positive for myosin VIIA, nestin and beta III tubulin.
Utricular epithelia of seven days old mice contains sufficient pluripotent stem cells which generate spheres.
Cells obtained from utricular epithelia are pluripotent because they express nanog, oct 4, nestin, characteristic for cell progenitors. |
| first_indexed | 2024-04-24T11:22:48Z |
| format | Article |
| id | doaj.art-c8980f5540b74990bd706a8dd78f1677 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:22:48Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-c8980f5540b74990bd706a8dd78f16772024-04-11T04:13:08ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01451358358604Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived SpheresRoxana Vintilă0Oana Gavriliuc1University of Medicine and Pharmacy “Victor Babes” Timisoara, Romania Banat’s University of Agricultural Sciences and Veterinary Medicine from Timişoara, 300645-Timisoara, Calea Aradului, 119, RomaniaThe purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the vestibular epithelia of young mice. We harvested utricles and sacculi from 7 days old mice. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM with F12 Nutrient mixture, B27, N2 supplement, IGF-1 and EGF. Sphere pluripotency was established with Nanog and Oct-4 stem cell markers. We mechanically dissociated primary spheres and cultivated them. Cells were characterized by immunofluorescence and immunohistochemistry for myosinVIIA (hair cell marker), nestin (intermediate filament VI marker) and beta III tubulin. We proved that vestibular epithelia contains pluripotent stem cells which formed spheres. Sphere-derived cells’ pluripotency was demonstrated by the expression of nanog, oct 4 and nestin markers. Also, after sphere dissociation we obtained a higher number of spheres beeing pluripotent and capable of self-renewal. We obtained through differentiation different cell types including neuron like-cells which were positive for myosin VIIA, nestin and beta III tubulin. Utricular epithelia of seven days old mice contains sufficient pluripotent stem cells which generate spheres. Cells obtained from utricular epithelia are pluripotent because they express nanog, oct 4, nesThe purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the vestibular epithelia of young mice. We harvested utricles and sacculi from 7 days old mice. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM with F12 Nutrient mixture, B27, N2 supplement, IGF-1 and EGF. Sphere pluripotency was established with Nanog and Oct-4 stem cell markers. We mechanically dissociated primary spheres and cultivated them. Cells were characterized by immunofluorescence and immunohistochemistry for myosinVIIA (hair cell marker), nestin (intermediate filament VI marker) and beta III tubulin. We proved that vestibular epithelia contains pluripotent stem cells which formed spheres. Sphere-derived cells’ pluripotency was demonstrated by the expression of nanog, oct 4 and nestin markers. Also, after sphere dissociation we obtained a higher number of spheres beeing pluripotent and capable of self-renewal. We obtained through differentiation different cell types including neuron like-cells which were positive for myosin VIIA, nestin and beta III tubulin. Utricular epithelia of seven days old mice contains sufficient pluripotent stem cells which generate spheres. Cells obtained from utricular epithelia are pluripotent because they express nanog, oct 4, nestin, characteristic for cell progenitors.https://spasb.ro/index.php/public_html/article/view/604inner earpluripotencystem cells |
| spellingShingle | Roxana Vintilă Oana Gavriliuc Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres Scientific Papers Animal Science and Biotechnologies inner ear pluripotency stem cells |
| title | Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres |
| title_full | Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres |
| title_fullStr | Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres |
| title_full_unstemmed | Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres |
| title_short | Results Regarding Self Renewal and Differentiation of Cells Composing Inner Ear Derived Spheres |
| title_sort | results regarding self renewal and differentiation of cells composing inner ear derived spheres |
| topic | inner ear pluripotency stem cells |
| url | https://spasb.ro/index.php/public_html/article/view/604 |
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