Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection
Trichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for Trichophyton and developed a LAMP-Microfluidic chip detection system for Trichophyton...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1031388/full |
| _version_ | 1797995958679633920 |
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| author | Weiwei Jiang Dongying Hu Yanyan Xu Yang Chen Xiaoyang Zhu Zhao Han Xin Ye Xiaojing Li |
| author_facet | Weiwei Jiang Dongying Hu Yanyan Xu Yang Chen Xiaoyang Zhu Zhao Han Xin Ye Xiaojing Li |
| author_sort | Weiwei Jiang |
| collection | DOAJ |
| description | Trichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for Trichophyton and developed a LAMP-Microfluidic chip detection system for Trichophyton. This system detects six common species of Trichophyton in the genus Trichophyton, including Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton schoenleinii. The specificity reached 100%, and the sensitivity could reach about 1 × 102 copies/μl. The entire detection process can be completed within 60 min and does not cross-react with other dermatophytes. The established LAMP-Microfluidic chip detection system has the advantages of simple operation, high specificity, and high sensitivity, and has the potential for clinical application. |
| first_indexed | 2024-04-11T10:08:45Z |
| format | Article |
| id | doaj.art-c8981812c4034ccbbdca267546d8dc6d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T10:08:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c8981812c4034ccbbdca267546d8dc6d2022-12-22T04:30:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10313881031388Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infectionWeiwei Jiang0Dongying Hu1Yanyan Xu2Yang Chen3Xiaoyang Zhu4Zhao Han5Xin Ye6Xiaojing Li7Department of Dermatology, 72nd Group Army Hospital of PLA, Huzhou, Zhejiang, ChinaDepartment of Dermatology, 72nd Group Army Hospital of PLA, Huzhou, Zhejiang, ChinaAffiliated Hospital of Hebei University of Engineering, Handan, Hebei, ChinaDepartment of Dermatology, 72nd Group Army Hospital of PLA, Huzhou, Zhejiang, ChinaDepartment of Dermatology, 72nd Group Army Hospital of PLA, Huzhou, Zhejiang, ChinaAffiliated Hospital of Hebei University of Engineering, Handan, Hebei, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaAffiliated Hospital of Hebei University of Engineering, Handan, Hebei, ChinaTrichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for Trichophyton and developed a LAMP-Microfluidic chip detection system for Trichophyton. This system detects six common species of Trichophyton in the genus Trichophyton, including Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton schoenleinii. The specificity reached 100%, and the sensitivity could reach about 1 × 102 copies/μl. The entire detection process can be completed within 60 min and does not cross-react with other dermatophytes. The established LAMP-Microfluidic chip detection system has the advantages of simple operation, high specificity, and high sensitivity, and has the potential for clinical application.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1031388/fullTrichophytonLAMPmicrofluidicsearly diagnosischip |
| spellingShingle | Weiwei Jiang Dongying Hu Yanyan Xu Yang Chen Xiaoyang Zhu Zhao Han Xin Ye Xiaojing Li Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection Frontiers in Microbiology Trichophyton LAMP microfluidics early diagnosis chip |
| title | Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection |
| title_full | Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection |
| title_fullStr | Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection |
| title_full_unstemmed | Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection |
| title_short | Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection |
| title_sort | loop mediated isothermal amplification microfluidic chip for the detection of trichophyton infection |
| topic | Trichophyton LAMP microfluidics early diagnosis chip |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1031388/full |
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