Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis
Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untrea...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2012-10-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017 |
| _version_ | 1819229148352086016 |
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| author | Luciana Cristina Silveira Chaud Débora Danielle Virgínio da Silva Rafael Taino de Mattos Maria das Graças de Almeida Felipe |
| author_facet | Luciana Cristina Silveira Chaud Débora Danielle Virgínio da Silva Rafael Taino de Mattos Maria das Graças de Almeida Felipe |
| author_sort | Luciana Cristina Silveira Chaud |
| collection | DOAJ |
| description | Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to presence of high concentration of toxic compounds. Thus, further studies are suggested in order to determine the best conditions for hydrolysis and detoxification of the hydrolysate to improve the fermentative performance of P. stipitis. |
| first_indexed | 2024-12-23T11:08:34Z |
| format | Article |
| id | doaj.art-c89a0ae8fb7c45e78d5926cd057ee988 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T11:08:34Z |
| publishDate | 2012-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-c89a0ae8fb7c45e78d5926cd057ee9882022-12-21T17:49:25ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242012-10-0155577177710.1590/S1516-89132012000500017Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitisLuciana Cristina Silveira ChaudDébora Danielle Virgínio da SilvaRafael Taino de MattosMaria das Graças de Almeida FelipeOat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to presence of high concentration of toxic compounds. Thus, further studies are suggested in order to determine the best conditions for hydrolysis and detoxification of the hydrolysate to improve the fermentative performance of P. stipitis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017Pichia stipitishemicellulosic hydrolysateoat hullethanolactivated charcoal |
| spellingShingle | Luciana Cristina Silveira Chaud Débora Danielle Virgínio da Silva Rafael Taino de Mattos Maria das Graças de Almeida Felipe Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis Brazilian Archives of Biology and Technology Pichia stipitis hemicellulosic hydrolysate oat hull ethanol activated charcoal |
| title | Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis |
| title_full | Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis |
| title_fullStr | Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis |
| title_full_unstemmed | Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis |
| title_short | Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis |
| title_sort | evaluation of oat hull hemicellulosic hydrolysate fermentability employing pichia stipitis |
| topic | Pichia stipitis hemicellulosic hydrolysate oat hull ethanol activated charcoal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017 |
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