An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini
This study aims to develop a fast and eco-friendly liquid chromatography–mass spectrometry (LC-MS) method for the determination of aesculin and aesculetin in Cortex Fraxini. Ultrapure water was used as the solvent during the microwave-assisted extraction process to prepare the Cortex Fraxini sample....
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| Format: | Article |
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/9/515 |
| _version_ | 1827723509022851072 |
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| author | Xiaodong Wang Wenhao Wang Qinggui Lei Zhengming Qian Wenbin Deng |
| author_facet | Xiaodong Wang Wenhao Wang Qinggui Lei Zhengming Qian Wenbin Deng |
| author_sort | Xiaodong Wang |
| collection | DOAJ |
| description | This study aims to develop a fast and eco-friendly liquid chromatography–mass spectrometry (LC-MS) method for the determination of aesculin and aesculetin in Cortex Fraxini. Ultrapure water was used as the solvent during the microwave-assisted extraction process to prepare the Cortex Fraxini sample. This extraction method reduces the cost of the harmful solvent (only ultrapure water was used) and microwave extraction time (1 min). The LC separation was conducted using an Agilent InfinityLab Poroshell 120 EC-C<sub>18</sub> column (2.1 mm × 30 mm, 2.7 µm) with a mobile phase consisting of 0.1% formic acid and acetonitrile (90:10, <i>v</i>/<i>v</i>) at a flow rate of 0.6 mL/min. Isocratic elution was employed, and the analytes were detected by MS. Through careful optimization and selection of LC-MS conditions, the analysis time was reduced to 1 min, demonstrating the method’s efficiency. The developed method was validated and exhibited excellent specificity, linearity, limit, precision, accuracy, and stability in quantifying aesculin and aesculetin in the Cortex Fraxini samples. The analysis result revealed the presence of aesculin (ranging from 3.55 to 18.8 mg/g) and aesculetin (ranging from 1.01 to 16.2 mg/g) in all ten batches of Cortex Fraxini samples. Compared to the reported LC methods, this approach substantially reduces the total analysis time and requires a minuscule volume of organic solvents. An “Analytical Eco-Scale” assessment was used to evaluate the different assay methods of Cortex Fraxini. The current LC-MS method scored an impressive 90; it was better than the other four reports’ LC methods. Thus, the developed LC-MS method is rapid and green, which is helpful for the quality evaluation of Cortex Fraxini. |
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| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T22:00:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-c8a0a7279419474f80fff1542936ef012023-11-19T12:58:13ZengMDPI AGSeparations2297-87392023-09-0110951510.3390/separations10090515An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex FraxiniXiaodong Wang0Wenhao Wang1Qinggui Lei2Zhengming Qian3Wenbin Deng4School of Pharmaceutical Sciences, Sun Yat-sen University, No.66, Gongchang Road, Guangming District, Shenzhen 518107, ChinaKey Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., No.368, Zhen’an Middle Road, Chang’an Town, Dongguan 523850, ChinaKey Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., No.368, Zhen’an Middle Road, Chang’an Town, Dongguan 523850, ChinaKey Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., No.368, Zhen’an Middle Road, Chang’an Town, Dongguan 523850, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, No.66, Gongchang Road, Guangming District, Shenzhen 518107, ChinaThis study aims to develop a fast and eco-friendly liquid chromatography–mass spectrometry (LC-MS) method for the determination of aesculin and aesculetin in Cortex Fraxini. Ultrapure water was used as the solvent during the microwave-assisted extraction process to prepare the Cortex Fraxini sample. This extraction method reduces the cost of the harmful solvent (only ultrapure water was used) and microwave extraction time (1 min). The LC separation was conducted using an Agilent InfinityLab Poroshell 120 EC-C<sub>18</sub> column (2.1 mm × 30 mm, 2.7 µm) with a mobile phase consisting of 0.1% formic acid and acetonitrile (90:10, <i>v</i>/<i>v</i>) at a flow rate of 0.6 mL/min. Isocratic elution was employed, and the analytes were detected by MS. Through careful optimization and selection of LC-MS conditions, the analysis time was reduced to 1 min, demonstrating the method’s efficiency. The developed method was validated and exhibited excellent specificity, linearity, limit, precision, accuracy, and stability in quantifying aesculin and aesculetin in the Cortex Fraxini samples. The analysis result revealed the presence of aesculin (ranging from 3.55 to 18.8 mg/g) and aesculetin (ranging from 1.01 to 16.2 mg/g) in all ten batches of Cortex Fraxini samples. Compared to the reported LC methods, this approach substantially reduces the total analysis time and requires a minuscule volume of organic solvents. An “Analytical Eco-Scale” assessment was used to evaluate the different assay methods of Cortex Fraxini. The current LC-MS method scored an impressive 90; it was better than the other four reports’ LC methods. Thus, the developed LC-MS method is rapid and green, which is helpful for the quality evaluation of Cortex Fraxini.https://www.mdpi.com/2297-8739/10/9/515LC-MSCortex Fraxiniaesculinaesculetingreenfast |
| spellingShingle | Xiaodong Wang Wenhao Wang Qinggui Lei Zhengming Qian Wenbin Deng An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini Separations LC-MS Cortex Fraxini aesculin aesculetin green fast |
| title | An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini |
| title_full | An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini |
| title_fullStr | An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini |
| title_full_unstemmed | An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini |
| title_short | An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini |
| title_sort | ultra fast and green lc ms method for quantitative analysis of aesculin and aesculetin in cortex fraxini |
| topic | LC-MS Cortex Fraxini aesculin aesculetin green fast |
| url | https://www.mdpi.com/2297-8739/10/9/515 |
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