Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes
Human papillomavirus (HPV) in high-risk groups is known to suppress the type I interferon (IFN) signaling pathway leading to the transcription of interferon-stimulated genes (ISGs), which have many antiviral functions. However, the effects of HPV on the action of various ISGs in low-risk groups are...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2418 |
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| author | Ji Young Song Ju Hee Han Yumee Song Ji Hyun Lee Soon Yong Choi Young Min Park |
| author_facet | Ji Young Song Ju Hee Han Yumee Song Ji Hyun Lee Soon Yong Choi Young Min Park |
| author_sort | Ji Young Song |
| collection | DOAJ |
| description | Human papillomavirus (HPV) in high-risk groups is known to suppress the type I interferon (IFN) signaling pathway leading to the transcription of interferon-stimulated genes (ISGs), which have many antiviral functions. However, the effects of HPV on the action of various ISGs in low-risk groups are not fully understood. We aimed to investigate whether antiviral ISGs are expressed in transfected keratinocytes with type 2 HPV (HPV-2) <i>E7</i>. The mRNA and protein expressions of ISGs and type I IFN signaling pathway components were evaluated by quantitative real-time polymerase chain reaction, western blot, immunofluorescence, and/or immunohistochemistry. Compared with normal skin, mRNA expression of all ISGs in HPV-2 positive cutaneous warts was significantly decreased (<i>p</i> < 0.05). In comparison with empty vector transfection, <i>E7</i> transfection significantly down-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly up-regulated by <i>E7</i> siRNA transfection (<i>p</i> < 0.05). Interestingly, epigallocatechin-3-gallate (EGCG) pretreatment up-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly down-regulated by <i>E7</i> transfection (<i>p</i> < 0.05). Our results demonstrate that EGCG is a potential candidate for cutaneous wart prevention. |
| first_indexed | 2024-03-09T06:14:01Z |
| format | Article |
| id | doaj.art-c8a1574790fc4ad2bf3a89282f955884 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T06:14:01Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c8a1574790fc4ad2bf3a89282f9558842023-12-03T11:55:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01225241810.3390/ijms22052418Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated GenesJi Young Song0Ju Hee Han1Yumee Song2Ji Hyun Lee3Soon Yong Choi4Young Min Park5Program of Immunology & Microbiology, Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, KoreaDepartment of Dermatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, KoreaDepartment of Dermatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, KoreaDepartment of Dermatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, KoreaDepartment of Biological Science and Biotechnology, College of Life Science and Nano Technology, Hannam University, 1646, Yuseong-daero, Yuseong-gu, Daejeon 34054, KoreaProgram of Immunology & Microbiology, Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, KoreaHuman papillomavirus (HPV) in high-risk groups is known to suppress the type I interferon (IFN) signaling pathway leading to the transcription of interferon-stimulated genes (ISGs), which have many antiviral functions. However, the effects of HPV on the action of various ISGs in low-risk groups are not fully understood. We aimed to investigate whether antiviral ISGs are expressed in transfected keratinocytes with type 2 HPV (HPV-2) <i>E7</i>. The mRNA and protein expressions of ISGs and type I IFN signaling pathway components were evaluated by quantitative real-time polymerase chain reaction, western blot, immunofluorescence, and/or immunohistochemistry. Compared with normal skin, mRNA expression of all ISGs in HPV-2 positive cutaneous warts was significantly decreased (<i>p</i> < 0.05). In comparison with empty vector transfection, <i>E7</i> transfection significantly down-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly up-regulated by <i>E7</i> siRNA transfection (<i>p</i> < 0.05). Interestingly, epigallocatechin-3-gallate (EGCG) pretreatment up-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly down-regulated by <i>E7</i> transfection (<i>p</i> < 0.05). Our results demonstrate that EGCG is a potential candidate for cutaneous wart prevention.https://www.mdpi.com/1422-0067/22/5/2418<i>E7</i>epigallocatechin-3-gallatehuman papillomavirusinterferon-stimulated genestype I IFN signaling pathway |
| spellingShingle | Ji Young Song Ju Hee Han Yumee Song Ji Hyun Lee Soon Yong Choi Young Min Park Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes International Journal of Molecular Sciences <i>E7</i> epigallocatechin-3-gallate human papillomavirus interferon-stimulated genes type I IFN signaling pathway |
| title | Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes |
| title_full | Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes |
| title_fullStr | Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes |
| title_full_unstemmed | Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes |
| title_short | Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus <i>E7</i> from Suppressing Interferon-Stimulated Genes |
| title_sort | epigallocatechin 3 gallate can prevent type 2 human papillomavirus i e7 i from suppressing interferon stimulated genes |
| topic | <i>E7</i> epigallocatechin-3-gallate human papillomavirus interferon-stimulated genes type I IFN signaling pathway |
| url | https://www.mdpi.com/1422-0067/22/5/2418 |
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