Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel
Regeneration of periodontal tissues requires an integrated approach to the restoration of the periodontal ligament, cementum, and alveolar bone surrounding the teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, in particular the decellularized extracellular matri...
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MDPI AG
2023-09-01
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| author | Alexey A. Ivanov Alla V. Kuznetsova Olga P. Popova Tamara I. Danilova Andrey V. Latyshev Oleg O. Yanushevich |
| author_facet | Alexey A. Ivanov Alla V. Kuznetsova Olga P. Popova Tamara I. Danilova Andrey V. Latyshev Oleg O. Yanushevich |
| author_sort | Alexey A. Ivanov |
| collection | DOAJ |
| description | Regeneration of periodontal tissues requires an integrated approach to the restoration of the periodontal ligament, cementum, and alveolar bone surrounding the teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, in particular the decellularized extracellular matrix (dECM), to facilitate the recruitment of populations of resident cells into damaged tissues and stimulate their proliferation and differentiation. The purpose of our study was to evaluate the effect of the exogenous components of the extracellular matrix (hyaluronic acid, laminin, fibronectin) on the differentiation of periodontal ligament stem cells (PDLSCs) cultured with dECM (combinations of decellularized tooth matrices and periodontal ligament) in a 3D collagen I hydrogel. The immunohistochemical expression of various markers in PDLSCs was assessed quantitatively and semi-quantitatively on paraffin sections. The results showed that PDLSCs cultured under these conditions for 14 days exhibited phenotypic characteristics consistent with osteoblast-like and odontoblast-like cells. This potential has been demonstrated by the expression of osteogenic differentiation markers (OC, OPN, ALP) and odontogenic markers (DSPP). This phenomenon corresponds to the in vivo state of the periodontal ligament, in which cells at the interface between bone and cementum tend to differentiate into osteoblasts or cementoblasts. The addition of fibronectin to the dECM most effectively induces the differentiation of PDLSCs into osteoblast-like and odontoblast-like cells under 3D culture conditions. Therefore, this bioengineered construct has a high potential for future use in periodontal tissue regeneration. |
| first_indexed | 2024-03-10T21:47:59Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T21:47:59Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-c8a22ea73454438588e38dcb9980d35f2023-11-19T14:12:19ZengMDPI AGCells2073-44092023-09-011219233510.3390/cells12192335Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I HydrogelAlexey A. Ivanov0Alla V. Kuznetsova1Olga P. Popova2Tamara I. Danilova3Andrey V. Latyshev4Oleg O. Yanushevich5Laboratory of Molecular and Cellular Pathology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaLaboratory of Molecular and Cellular Pathology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaLaboratory of Molecular and Cellular Pathology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaLaboratory of Molecular and Cellular Pathology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaLaboratory of Molecular and Cellular Pathology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaDepartment of Periodontology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 20 Delegatskaya Str., 127473 Moscow, RussiaRegeneration of periodontal tissues requires an integrated approach to the restoration of the periodontal ligament, cementum, and alveolar bone surrounding the teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, in particular the decellularized extracellular matrix (dECM), to facilitate the recruitment of populations of resident cells into damaged tissues and stimulate their proliferation and differentiation. The purpose of our study was to evaluate the effect of the exogenous components of the extracellular matrix (hyaluronic acid, laminin, fibronectin) on the differentiation of periodontal ligament stem cells (PDLSCs) cultured with dECM (combinations of decellularized tooth matrices and periodontal ligament) in a 3D collagen I hydrogel. The immunohistochemical expression of various markers in PDLSCs was assessed quantitatively and semi-quantitatively on paraffin sections. The results showed that PDLSCs cultured under these conditions for 14 days exhibited phenotypic characteristics consistent with osteoblast-like and odontoblast-like cells. This potential has been demonstrated by the expression of osteogenic differentiation markers (OC, OPN, ALP) and odontogenic markers (DSPP). This phenomenon corresponds to the in vivo state of the periodontal ligament, in which cells at the interface between bone and cementum tend to differentiate into osteoblasts or cementoblasts. The addition of fibronectin to the dECM most effectively induces the differentiation of PDLSCs into osteoblast-like and odontoblast-like cells under 3D culture conditions. Therefore, this bioengineered construct has a high potential for future use in periodontal tissue regeneration.https://www.mdpi.com/2073-4409/12/19/2335decellularized extracellular matrixperiodontal ligament stem cellscollagen I hydrogelhyaluronic acidfibronectinlaminin |
| spellingShingle | Alexey A. Ivanov Alla V. Kuznetsova Olga P. Popova Tamara I. Danilova Andrey V. Latyshev Oleg O. Yanushevich Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel Cells decellularized extracellular matrix periodontal ligament stem cells collagen I hydrogel hyaluronic acid fibronectin laminin |
| title | Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel |
| title_full | Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel |
| title_fullStr | Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel |
| title_full_unstemmed | Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel |
| title_short | Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel |
| title_sort | influence of extracellular matrix components on the differentiation of periodontal ligament stem cells in collagen i hydrogel |
| topic | decellularized extracellular matrix periodontal ligament stem cells collagen I hydrogel hyaluronic acid fibronectin laminin |
| url | https://www.mdpi.com/2073-4409/12/19/2335 |
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