Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i>
Premise of the study: Microsatellite markers were developed and characterized in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) to allow further investigation of genetic variation in natural populations. Cross-amplification was tested in the related species <i>B. crassifolia</i&g...
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| Format: | Article |
| Language: | English |
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Wiley
2014-05-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1400016 |
| _version_ | 1818986763271536640 |
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| author | Vanessa Bernardes Daniela Elaine dos Anjos Sara Giselle de Cássia Alexandre Gondim Devanir Mitsuyuki Murakami Nair Bizão Mariana Pires de Campos Telles |
| author_facet | Vanessa Bernardes Daniela Elaine dos Anjos Sara Giselle de Cássia Alexandre Gondim Devanir Mitsuyuki Murakami Nair Bizão Mariana Pires de Campos Telles |
| author_sort | Vanessa Bernardes |
| collection | DOAJ |
| description | Premise of the study: Microsatellite markers were developed and characterized in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) to allow further investigation of genetic variation in natural populations. Cross-amplification was tested in the related species <i>B. crassifolia</i>.
Methods and Results: Seventeen microsatellite markers were isolated by a microsatellite-enriched library protocol. Fourteen polymorphic and three monomorphic loci were identified in <i>B. cydoniifolia</i>. The mean number of alleles in the three populations were 6.5, 6.5, and 8.2, ranging from three to 17 for different loci and populations. Mean observed and expected heterozygosities were 0.706 and 0.727, respectively. The fixation index was close to zero for all but two loci. Nine microsatellite loci were successfully cross-amplified in <i>B. crassifolia</i>.
Conclusions: This new set of microsatellite markers will be a useful tool for genetic studies of <i>B. cydoniifolia</i>, supporting strategies for maintaining the genetic diversity of this species and possibly that of many related species. |
| first_indexed | 2024-12-20T18:55:57Z |
| format | Article |
| id | doaj.art-c8a500fb1fdc41e58ad3c251292e0172 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-20T18:55:57Z |
| publishDate | 2014-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-c8a500fb1fdc41e58ad3c251292e01722022-12-21T19:29:30ZengWileyApplications in Plant Sciences2168-04502014-05-0125140001610.3732/apps.1400016Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i>Vanessa Bernardes0Daniela Elaine dos Anjos1Sara Giselle de Cássia Alexandre Gondim2Devanir Mitsuyuki Murakami3Nair Bizão4Mariana Pires de Campos Telles5Programa de Pós-graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil; Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, Barra do Garças CP 78600-000, Mato Grosso, BrazilInstituto de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso, Barra do Garças CP 78600-000, Mato Grosso, BrazilPrograma de Pós-graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil; Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil Premise of the study: Microsatellite markers were developed and characterized in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) to allow further investigation of genetic variation in natural populations. Cross-amplification was tested in the related species <i>B. crassifolia</i>. Methods and Results: Seventeen microsatellite markers were isolated by a microsatellite-enriched library protocol. Fourteen polymorphic and three monomorphic loci were identified in <i>B. cydoniifolia</i>. The mean number of alleles in the three populations were 6.5, 6.5, and 8.2, ranging from three to 17 for different loci and populations. Mean observed and expected heterozygosities were 0.706 and 0.727, respectively. The fixation index was close to zero for all but two loci. Nine microsatellite loci were successfully cross-amplified in <i>B. crassifolia</i>. Conclusions: This new set of microsatellite markers will be a useful tool for genetic studies of <i>B. cydoniifolia</i>, supporting strategies for maintaining the genetic diversity of this species and possibly that of many related species.http://www.bioone.org/doi/full/10.3732/apps.1400016Byrsonima crassifoliaByrsonima cydoniifoliagenetic variabilityMalpighiaceaemuricisimple sequence repeat (SSR) |
| spellingShingle | Vanessa Bernardes Daniela Elaine dos Anjos Sara Giselle de Cássia Alexandre Gondim Devanir Mitsuyuki Murakami Nair Bizão Mariana Pires de Campos Telles Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> Applications in Plant Sciences Byrsonima crassifolia Byrsonima cydoniifolia genetic variability Malpighiaceae murici simple sequence repeat (SSR) |
| title | Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> |
| title_full | Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> |
| title_fullStr | Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> |
| title_full_unstemmed | Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> |
| title_short | Isolation and Characterization of Microsatellite Loci in <i>Byrsonima cydoniifolia</i> (Malpighiaceae) and Cross-Amplification in <i>B. crassifolia</i> |
| title_sort | isolation and characterization of microsatellite loci in i byrsonima cydoniifolia i malpighiaceae and cross amplification in i b crassifolia i |
| topic | Byrsonima crassifolia Byrsonima cydoniifolia genetic variability Malpighiaceae murici simple sequence repeat (SSR) |
| url | http://www.bioone.org/doi/full/10.3732/apps.1400016 |
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