Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats
Reproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes...
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/873 |
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| author | Joanna M.G. Souza-Fabjan Lucas F.L. Correia Ribrio I.T.P. Batista Yann Locatelli Vicente J.F. Freitas Pascal Mermillod |
| author_facet | Joanna M.G. Souza-Fabjan Lucas F.L. Correia Ribrio I.T.P. Batista Yann Locatelli Vicente J.F. Freitas Pascal Mermillod |
| author_sort | Joanna M.G. Souza-Fabjan |
| collection | DOAJ |
| description | Reproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes (COCs) were recovered from slaughterhouse ovaries and subjected to the same IVP system throughout 1.5 years (49 replicates). The odds ratio (OR) among seasons was calculated from values of cleavage and blastocyst rates in each season. Cleavage rate was lower (<i>p</i> < 0.05) in spring (anestrus), in comparison with either autumn (peak of breeding season) or summer, while the winter had intermediate values. Furthermore, lower OR of cleavage was observed in spring. Blastocyst formation rate (from initial number of COCs) was higher (<i>p</i> < 0.05) in autumn (52 ± 2.5%) when compared with the other seasons (combined rates: 40 ± 1.9%). Moreover, its OR was higher (<i>p</i> < 0.05) in autumn compared to all other seasons and impaired in the spring compared to winter (OR: 0.54) and summer (OR: 0.48). Embryo hatchability and blastocyst cell number were similar (<i>p</i> > 0.05) among seasons. In conclusion, the breeding season leads to improved oocyte developmental competence, resulting in higher cleavage and blastocyst yield, whereas embryo quality remained similar throughout the years. |
| first_indexed | 2024-03-10T13:07:10Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:07:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c8a50ebfb30043b79decb8795ef41e4b2023-11-21T11:04:59ZengMDPI AGAnimals2076-26152021-03-0111387310.3390/ani11030873Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult GoatsJoanna M.G. Souza-Fabjan0Lucas F.L. Correia1Ribrio I.T.P. Batista2Yann Locatelli3Vicente J.F. Freitas4Pascal Mermillod5Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ 24230-340, BrazilFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ 24230-340, BrazilFaculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ 24230-340, BrazilMuseum National d’Histoire Naturelle, Réserve Zoologique de la Haute Touche, 36290 Obterre, FranceLaboratório de Fisiologia e Controle da Reprodução, Universidade Estadual do Ceará, Fortaleza, CE 60714-903, BrazilInstitut National de Recherche Pour L’agriculture, L’alimentation et L’environnement (INRAE), Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, FranceReproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes (COCs) were recovered from slaughterhouse ovaries and subjected to the same IVP system throughout 1.5 years (49 replicates). The odds ratio (OR) among seasons was calculated from values of cleavage and blastocyst rates in each season. Cleavage rate was lower (<i>p</i> < 0.05) in spring (anestrus), in comparison with either autumn (peak of breeding season) or summer, while the winter had intermediate values. Furthermore, lower OR of cleavage was observed in spring. Blastocyst formation rate (from initial number of COCs) was higher (<i>p</i> < 0.05) in autumn (52 ± 2.5%) when compared with the other seasons (combined rates: 40 ± 1.9%). Moreover, its OR was higher (<i>p</i> < 0.05) in autumn compared to all other seasons and impaired in the spring compared to winter (OR: 0.54) and summer (OR: 0.48). Embryo hatchability and blastocyst cell number were similar (<i>p</i> > 0.05) among seasons. In conclusion, the breeding season leads to improved oocyte developmental competence, resulting in higher cleavage and blastocyst yield, whereas embryo quality remained similar throughout the years.https://www.mdpi.com/2076-2615/11/3/873caprineCOCIVFIVPIVEPoocyte competence |
| spellingShingle | Joanna M.G. Souza-Fabjan Lucas F.L. Correia Ribrio I.T.P. Batista Yann Locatelli Vicente J.F. Freitas Pascal Mermillod Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats Animals caprine COC IVF IVP IVEP oocyte competence |
| title | Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats |
| title_full | Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats |
| title_fullStr | Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats |
| title_full_unstemmed | Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats |
| title_short | Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats |
| title_sort | reproductive seasonality affects in vitro embryo production outcomes in adult goats |
| topic | caprine COC IVF IVP IVEP oocyte competence |
| url | https://www.mdpi.com/2076-2615/11/3/873 |
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