Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
Summary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001982 |
| _version_ | 1823941126673924096 |
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| author | Giacomo Canesin Lubica Janovicova Barbara Wegiel |
| author_facet | Giacomo Canesin Lubica Janovicova Barbara Wegiel |
| author_sort | Giacomo Canesin |
| collection | DOAJ |
| description | Summary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments in vitro. The protocol can be adapted to be used for other cell types.For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020). |
| first_indexed | 2024-12-17T04:23:16Z |
| format | Article |
| id | doaj.art-c8aa03b2f940427a85dac339e21c0f71 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T04:23:16Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c8aa03b2f940427a85dac339e21c0f712022-12-21T22:03:47ZengElsevierSTAR Protocols2666-16672021-06-0122100491Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractionsGiacomo Canesin0Lubica Janovicova1Barbara Wegiel2Department of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USADepartment of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USADepartment of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USA; Corresponding authorSummary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments in vitro. The protocol can be adapted to be used for other cell types.For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001982Cell BiologyCell-based AssaysCell separation/fractionationCancerProtein Biochemistry |
| spellingShingle | Giacomo Canesin Lubica Janovicova Barbara Wegiel Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions STAR Protocols Cell Biology Cell-based Assays Cell separation/fractionation Cancer Protein Biochemistry |
| title | Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions |
| title_full | Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions |
| title_fullStr | Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions |
| title_full_unstemmed | Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions |
| title_short | Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions |
| title_sort | measurement of labile and protein bound heme in fixed prostate cancer cells and in cellular fractions |
| topic | Cell Biology Cell-based Assays Cell separation/fractionation Cancer Protein Biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721001982 |
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