Diversity and epidemiology of Mokola virus.
Mokola virus (MOKV) appears to be exclusive to Africa. Although the first isolates were from Nigeria and other Congo basin countries, all reports over the past 20 years have been from southern Africa. Previous phylogenetic studies analyzed few isolates or used partial gene sequence for analysis sinc...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC3812115?pdf=render |
| _version_ | 1818197551960031232 |
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| author | Joe Kgaladi Nicolette Wright Jessica Coertse Wanda Markotter Denise Marston Anthony R Fooks Conrad M Freuling Thomas F Müller Claude T Sabeta Louis H Nel |
| author_facet | Joe Kgaladi Nicolette Wright Jessica Coertse Wanda Markotter Denise Marston Anthony R Fooks Conrad M Freuling Thomas F Müller Claude T Sabeta Louis H Nel |
| author_sort | Joe Kgaladi |
| collection | DOAJ |
| description | Mokola virus (MOKV) appears to be exclusive to Africa. Although the first isolates were from Nigeria and other Congo basin countries, all reports over the past 20 years have been from southern Africa. Previous phylogenetic studies analyzed few isolates or used partial gene sequence for analysis since limited sequence information is available for MOKV and the isolates were distributed among various laboratories. The complete nucleoprotein, phosphoprotein, matrix and glycoprotein genes of 18 MOKV isolates in various laboratories were sequenced either using partial or full genome sequencing using pyrosequencing and a phylogenetic analysis was undertaken. The results indicated that MOKV isolates from the Republic of South Africa, Zimbabwe, Central African Republic and Nigeria clustered according to geographic origin irrespective of the genes used for phylogenetic analysis, similar to that observed with Lagos bat virus. A Bayesian Markov-Chain-Monte-Carlo- (MCMC) analysis revealed the age of the most recent common ancestor (MRCA) of MOKV to be between 279 and 2034 years depending on the genes used. Generally, all MOKV isolates showed a similar pattern at the amino acid sites considered influential for viral properties. |
| first_indexed | 2024-12-12T01:51:47Z |
| format | Article |
| id | doaj.art-c8ab391c4f1f416ab2bf53e6c500cf62 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-12T01:51:47Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-c8ab391c4f1f416ab2bf53e6c500cf622022-12-22T00:42:27ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352013-01-01710e251110.1371/journal.pntd.0002511Diversity and epidemiology of Mokola virus.Joe KgaladiNicolette WrightJessica CoertseWanda MarkotterDenise MarstonAnthony R FooksConrad M FreulingThomas F MüllerClaude T SabetaLouis H NelMokola virus (MOKV) appears to be exclusive to Africa. Although the first isolates were from Nigeria and other Congo basin countries, all reports over the past 20 years have been from southern Africa. Previous phylogenetic studies analyzed few isolates or used partial gene sequence for analysis since limited sequence information is available for MOKV and the isolates were distributed among various laboratories. The complete nucleoprotein, phosphoprotein, matrix and glycoprotein genes of 18 MOKV isolates in various laboratories were sequenced either using partial or full genome sequencing using pyrosequencing and a phylogenetic analysis was undertaken. The results indicated that MOKV isolates from the Republic of South Africa, Zimbabwe, Central African Republic and Nigeria clustered according to geographic origin irrespective of the genes used for phylogenetic analysis, similar to that observed with Lagos bat virus. A Bayesian Markov-Chain-Monte-Carlo- (MCMC) analysis revealed the age of the most recent common ancestor (MRCA) of MOKV to be between 279 and 2034 years depending on the genes used. Generally, all MOKV isolates showed a similar pattern at the amino acid sites considered influential for viral properties.http://europepmc.org/articles/PMC3812115?pdf=render |
| spellingShingle | Joe Kgaladi Nicolette Wright Jessica Coertse Wanda Markotter Denise Marston Anthony R Fooks Conrad M Freuling Thomas F Müller Claude T Sabeta Louis H Nel Diversity and epidemiology of Mokola virus. PLoS Neglected Tropical Diseases |
| title | Diversity and epidemiology of Mokola virus. |
| title_full | Diversity and epidemiology of Mokola virus. |
| title_fullStr | Diversity and epidemiology of Mokola virus. |
| title_full_unstemmed | Diversity and epidemiology of Mokola virus. |
| title_short | Diversity and epidemiology of Mokola virus. |
| title_sort | diversity and epidemiology of mokola virus |
| url | http://europepmc.org/articles/PMC3812115?pdf=render |
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