Does flow culture impact upon gut-probiotic interactions: A comparison with static culture
Conventional tissue culture models often lack physiological relevance. In this exploratory study, dynamic flow conditions were applied to 21-day cultured Caco-2 intestinal epithelial cells and their response compared with statically cultured counterparts. Host:microbiome interactions were also explo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001196 |
| _version_ | 1797837122111012864 |
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| author | D.R. Michael J. Kerry-Smith T.S. Webberley K.R. Murphy S.F. Plummer L. Parry J.R. Marchesi |
| author_facet | D.R. Michael J. Kerry-Smith T.S. Webberley K.R. Murphy S.F. Plummer L. Parry J.R. Marchesi |
| author_sort | D.R. Michael |
| collection | DOAJ |
| description | Conventional tissue culture models often lack physiological relevance. In this exploratory study, dynamic flow conditions were applied to 21-day cultured Caco-2 intestinal epithelial cells and their response compared with statically cultured counterparts. Host:microbiome interactions were also explored through co-incubation with Gram positive bacteria (Lab4 probiotics) or the Gram positive cell wall component, lipoteichoic acid (LTA).Under flow, Caco-2 cells displayed increased viability, mucin production and pH tolerance compared to cells under static conditions. Measurement of secretion rates of the pro-inflammatory cytokine, interleukin-8, showed that cells under flow were unresponsive towards stimulation with Lab4 or LTA that was in complete contrast to inductions observed under static conditions. Given that Gram positive bacteria are well tolerated within the healthy gastrointestinal tract, these data suggest that flow conditions may improve the physiological relevance of Caco-2 cells. This preliminary study encourages further work exploring the impact of flow on cell-based in vitro models. |
| first_indexed | 2024-04-09T15:20:50Z |
| format | Article |
| id | doaj.art-c8ab4b1156064023bea9850b9a1722bf |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-09T15:20:50Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c8ab4b1156064023bea9850b9a1722bf2023-04-29T14:48:24ZengElsevierJournal of Functional Foods1756-46462023-05-01104105519Does flow culture impact upon gut-probiotic interactions: A comparison with static cultureD.R. Michael0J. Kerry-Smith1T.S. Webberley2K.R. Murphy3S.F. Plummer4L. Parry5J.R. Marchesi6Cultech Limited, Unit 2 Christchurch Road, Port Talbot, United KingdomCultech Limited, Unit 2 Christchurch Road, Port Talbot, United Kingdom; Corresponding author.Cultech Limited, Unit 2 Christchurch Road, Port Talbot, United Kingdom; Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United KingdomSchool of Biosciences, Cardiff University, Cardiff, United KingdomCultech Limited, Unit 2 Christchurch Road, Port Talbot, United KingdomEuropean Cancer Stem Cell Research Institute, School of Biosciences, Cardiff University, Cardiff, United Kingdom; Wales Cancer Research Centre, University Hospital of Wales, Cardiff, United KingdomDivision of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, United KingdomConventional tissue culture models often lack physiological relevance. In this exploratory study, dynamic flow conditions were applied to 21-day cultured Caco-2 intestinal epithelial cells and their response compared with statically cultured counterparts. Host:microbiome interactions were also explored through co-incubation with Gram positive bacteria (Lab4 probiotics) or the Gram positive cell wall component, lipoteichoic acid (LTA).Under flow, Caco-2 cells displayed increased viability, mucin production and pH tolerance compared to cells under static conditions. Measurement of secretion rates of the pro-inflammatory cytokine, interleukin-8, showed that cells under flow were unresponsive towards stimulation with Lab4 or LTA that was in complete contrast to inductions observed under static conditions. Given that Gram positive bacteria are well tolerated within the healthy gastrointestinal tract, these data suggest that flow conditions may improve the physiological relevance of Caco-2 cells. This preliminary study encourages further work exploring the impact of flow on cell-based in vitro models.http://www.sciencedirect.com/science/article/pii/S1756464623001196Caco-2FlowMucinInterleukin-8Probiotic |
| spellingShingle | D.R. Michael J. Kerry-Smith T.S. Webberley K.R. Murphy S.F. Plummer L. Parry J.R. Marchesi Does flow culture impact upon gut-probiotic interactions: A comparison with static culture Journal of Functional Foods Caco-2 Flow Mucin Interleukin-8 Probiotic |
| title | Does flow culture impact upon gut-probiotic interactions: A comparison with static culture |
| title_full | Does flow culture impact upon gut-probiotic interactions: A comparison with static culture |
| title_fullStr | Does flow culture impact upon gut-probiotic interactions: A comparison with static culture |
| title_full_unstemmed | Does flow culture impact upon gut-probiotic interactions: A comparison with static culture |
| title_short | Does flow culture impact upon gut-probiotic interactions: A comparison with static culture |
| title_sort | does flow culture impact upon gut probiotic interactions a comparison with static culture |
| topic | Caco-2 Flow Mucin Interleukin-8 Probiotic |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001196 |
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