Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria
The circadian clock interacts with other regulatory pathways to tune physiology to predictable daily changes and unexpected environmental fluctuations. However, the complexity of circadian clocks in higher organisms has prevented a clear understanding of how natural environmental conditions affect c...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2017-12-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/32032 |
| _version_ | 1811236837023285248 |
|---|---|
| author | Joseph Robert Piechura Kapil Amarnath Erin K O'Shea |
| author_facet | Joseph Robert Piechura Kapil Amarnath Erin K O'Shea |
| author_sort | Joseph Robert Piechura |
| collection | DOAJ |
| description | The circadian clock interacts with other regulatory pathways to tune physiology to predictable daily changes and unexpected environmental fluctuations. However, the complexity of circadian clocks in higher organisms has prevented a clear understanding of how natural environmental conditions affect circadian clocks and their physiological outputs. Here, we dissect the interaction between circadian regulation and responses to fluctuating light in the cyanobacterium Synechococcus elongatus. We demonstrate that natural changes in light intensity substantially affect the expression of hundreds of circadian-clock-controlled genes, many of which are involved in key steps of metabolism. These changes in expression arise from circadian and light-responsive control of RNA polymerase recruitment to promoters by a network of transcription factors including RpaA and RpaB. Using phenomenological modeling constrained by our data, we reveal simple principles that underlie the small number of stereotyped responses of dusk circadian genes to changes in light. |
| first_indexed | 2024-04-12T12:16:01Z |
| format | Article |
| id | doaj.art-c8b21dd081834a87bb84b2dc0ef2a5dd |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:16:01Z |
| publishDate | 2017-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c8b21dd081834a87bb84b2dc0ef2a5dd2022-12-22T03:33:26ZengeLife Sciences Publications LtdeLife2050-084X2017-12-01610.7554/eLife.32032Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteriaJoseph Robert Piechura0https://orcid.org/0000-0001-5349-4567Kapil Amarnath1https://orcid.org/0000-0003-2589-9684Erin K O'Shea2https://orcid.org/0000-0002-2649-1018Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States; FAS Center for Systems Biology, Harvard University, Cambridge, United States; Howard Hughes Medical Institute, Harvard University, Cambridge, United StatesFAS Center for Systems Biology, Harvard University, Cambridge, United States; Howard Hughes Medical Institute, Harvard University, Cambridge, United StatesDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, United States; FAS Center for Systems Biology, Harvard University, Cambridge, United States; Howard Hughes Medical Institute, Harvard University, Cambridge, United States; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United StatesThe circadian clock interacts with other regulatory pathways to tune physiology to predictable daily changes and unexpected environmental fluctuations. However, the complexity of circadian clocks in higher organisms has prevented a clear understanding of how natural environmental conditions affect circadian clocks and their physiological outputs. Here, we dissect the interaction between circadian regulation and responses to fluctuating light in the cyanobacterium Synechococcus elongatus. We demonstrate that natural changes in light intensity substantially affect the expression of hundreds of circadian-clock-controlled genes, many of which are involved in key steps of metabolism. These changes in expression arise from circadian and light-responsive control of RNA polymerase recruitment to promoters by a network of transcription factors including RpaA and RpaB. Using phenomenological modeling constrained by our data, we reveal simple principles that underlie the small number of stereotyped responses of dusk circadian genes to changes in light.https://elifesciences.org/articles/32032S. elongatuscircadian clockresponse regulatorscyanobacteriafluctuating lighttranscription networks |
| spellingShingle | Joseph Robert Piechura Kapil Amarnath Erin K O'Shea Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria eLife S. elongatus circadian clock response regulators cyanobacteria fluctuating light transcription networks |
| title | Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| title_full | Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| title_fullStr | Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| title_full_unstemmed | Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| title_short | Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| title_sort | natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria |
| topic | S. elongatus circadian clock response regulators cyanobacteria fluctuating light transcription networks |
| url | https://elifesciences.org/articles/32032 |
| work_keys_str_mv | AT josephrobertpiechura naturalchangesinlightinteractwithcircadianregulationatpromoterstocontrolgeneexpressionincyanobacteria AT kapilamarnath naturalchangesinlightinteractwithcircadianregulationatpromoterstocontrolgeneexpressionincyanobacteria AT erinkoshea naturalchangesinlightinteractwithcircadianregulationatpromoterstocontrolgeneexpressionincyanobacteria |